Enhancing Sustainable Rabbit Meat Production: A Feed-Based Strategy Using Natural Antioxidants to Improve Breeding Stock Fertility and Genetic Integrity | ||
| مجله علوم و صنایع غذایی ایران | ||
| Volume 22, Issue 164, 1404, Pages 231-240 PDF (375.81 K) | ||
| Document Type: مقاله پژوهشی | ||
| DOI: 10.48311/fsct.2026.118566.83011 | ||
| Authors | ||
| Noor Salman Dalis* 1; Bashaer Ibrahim Hamdi2; Raad Fadhil Abdullah Yosef2; Zaid khalid Alani3; Samer Mohammed Ismael Alsaffar4; Hiba Alaa Mohammed5 | ||
| 1Collage of Medicine, Physiology Department, University of Tikrit., | ||
| 2Pathological Analysis Department, College of Applied Sciences, University of Samarra, Samarra, Iraq. | ||
| 3College of Pharmacy, Al-Turath University, Baghdad, Iraq. | ||
| 4college of Dentistry, Al-Zahrawi University, Kerbala, Iraq. | ||
| 5Department of Medical Laboratory Techniques , College of Health and Medical Techniques , Al-Bayan University | ||
| Abstract | ||
| This study investigated the effects of natural antioxidant supplementation on semen quality parameters and sperm chromatin structure in rabbit bucks under oxidative stress conditions. Forty New Zealand White rabbit bucks (aged 7-9 months, 3.0-3.5 kg) were randomly allocated to four groups (n=10 per group): Control (C), Oxidative Stress (OS), OS + Natural Antioxidants (OS+NA), and Natural Antioxidants alone (NA). The natural antioxidant blend included vitamin E (200 mg/kg), organic selenium (0.3 mg/kg), vitamin C (500 mg/kg), and herbal extracts (150 mg/kg) for 90 days. Parameters assessed included sperm concentration, total and progressive motility, viability, morphological abnormalities, DNA fragmentation index (DFI), acrosome integrity, seminal plasma antioxidants (TAC, SOD, GPx, GSH, vitamins E and C, selenium), oxidative markers (MDA, ROS, 8-OHdG), and liver enzyme activities. Results showed oxidative stress significantly impaired progressive motility (47% reduction, P<0.001), viability (38% decrease, P<0.001), and dramatically increased DNA fragmentation (165% elevation, P<0.001). Natural antioxidants effectively restored progressive motility to 89% of control values (P<0.01), reduced DNA fragmentation by 58% versus OS group (P<0.001), and improved acrosome integrity by 43% (P<0.01). Seminal TAC increased 127% (P<0.001) while MDA decreased 62% (P<0.001) with antioxidant treatment. However, sperm concentration showed non-significant changes (P=0.186), and liver enzymes remained unaffected (P>0.05). Strong negative correlations were observed between seminal ROS and progressive motility (r=-0.81, P<0.001) and between DNA fragmentation and fertility markers (r=-0.76, P<0.001). This study demonstrates natural antioxidants effectively protect sperm DNA integrity and functional parameters through enhanced antioxidant defense, with DNA preservation representing the most dramatic protective effect. | ||
| Keywords | ||
| Natural antioxidants; semen quality; DNA fragmentation; oxidative stress; rabbit; selenium; vitamin E | ||
| References | ||
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