Al-Ayat, Adel Ahmed, Gharib, Ahmed Mohiy, Hassuba, Mahmoud Mohamed, Atta, Ayman Abdelwahab, Mesbah, Hassan Abdullah, Gad, Hassan Abdelrahim (2025). Effect of Entomopathogenic Nematode and Fungi on Mortality and Development of Spodoptera frugiperda (J. E. Smith) Larvae. , 27(2), 421-433. doi: 10.22034/JAST.27.2.421
Adel Ahmed Al-Ayat; Ahmed Mohiy Gharib; Mahmoud Mohamed Hassuba; Ayman Abdelwahab Atta; Hassan Abdullah Mesbah; Hassan Abdelrahim Gad. "Effect of Entomopathogenic Nematode and Fungi on Mortality and Development of Spodoptera frugiperda (J. E. Smith) Larvae". , 27, 2, 2025, 421-433. doi: 10.22034/JAST.27.2.421
Al-Ayat, Adel Ahmed, Gharib, Ahmed Mohiy, Hassuba, Mahmoud Mohamed, Atta, Ayman Abdelwahab, Mesbah, Hassan Abdullah, Gad, Hassan Abdelrahim (2025). 'Effect of Entomopathogenic Nematode and Fungi on Mortality and Development of Spodoptera frugiperda (J. E. Smith) Larvae', , 27(2), pp. 421-433. doi: 10.22034/JAST.27.2.421
Al-Ayat, Adel Ahmed, Gharib, Ahmed Mohiy, Hassuba, Mahmoud Mohamed, Atta, Ayman Abdelwahab, Mesbah, Hassan Abdullah, Gad, Hassan Abdelrahim Effect of Entomopathogenic Nematode and Fungi on Mortality and Development of Spodoptera frugiperda (J. E. Smith) Larvae. , 2025; 27(2): 421-433. doi: 10.22034/JAST.27.2.421

Effect of Entomopathogenic Nematode and Fungi on Mortality and Development of Spodoptera frugiperda (J. E. Smith) Larvae

Journal of Agricultural Science and Technology
Article 12, Volume 27, Issue 2, 2025, Pages 421-433    PDF (2.06 M)
Document Type: Original Research
DOI: 10.22034/JAST.27.2.421
Authors
Adel Ahmed Al-Ayat1; Ahmed Mohiy Gharib1; Mahmoud Mohamed Hassuba1; Ayman Abdelwahab Atta1; Hassan Abdullah Mesbah2; Hassan Abdelrahim Gad* 1
1Plant Protection Department, Faculty of Agriculture, Al-Azhar University, Cairo, Egypt.
2Department of Agricultural Botany (Genetics), Faculty of Agriculture, Al-Azhar University, Cairo, Egypt.
Abstract
The present study was conducted to evaluate the efficacy of entomopathogenic nematode, Steinernema carpocapsae Weiser and three entomopathogenic fungi (Metarhizium anisopliae (Metschn.) Sorokin, Trichoderma harzianum Rifai, and T. viride Pers.) against the second and fourth larval instars of Spodoptera frugiperda (J. E. Smith). The results showed that S. carpocapsae caused a pronounced mortality to second and fourth larval instars of S. frugiperda using a leaf dipping method at 4 days post inoculation (dpi) with LC50 values of 52.03 and 4.11 Infective Juveniles (IJs) mL-1, respectively. On the other hand, the three tested entomopathogenic fungi caused a strong toxicity on larval instars of S. frugiperda. The fungus, T. harzianum, displayed the highest insecticidal activity on the second larval instar (LC50= 1.1×107 spores mL-1) and M. anisopliae on the fourth larval instar (LC50= 1.5×107 spores mL-1) after 10 dpi. Our results showed that S. carpocapsae completely inhibited pupation and adult emergence from treated larvae at 250 IJs mL-1. The lethal effect of entomopathogenic nematode and fungi against S. frugiperda larval instars indicates that these biological control agents could be useful candidates in integrated pest management programs for this invasive insect.
Keywords
fall armyworm; Metarhizium anisopliae; Steinernema carpocapsae; Trichoderma harzianum
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