Differential Expression Profiles of Tomato miRNAs Induced by Tobacco Mosaic Virus | ||
| Journal of Agricultural Science and Technology | ||
| Article 19, Volume 21, Issue 2, 2019, Pages 475-485 PDF (810.15 K) | ||
| Authors | ||
| A. Abdelkhalek* 1; N. Sanan-Mishra2 | ||
| 1Plant Protection and Bimolecular Diagnosis Department, ALCRI, City of Scientific Research and Technological Applications, 21934, Alexandria, Egypt. | ||
| 2Plant RNAi Biology Group, International Center for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, India. | ||
| Abstract | ||
| Plant microRNAs (miRNAs) play important roles in plant development and responses to biotic and abiotic stress. Recently, there is clear evidence that miRNAs are involved in host-virus interactions. By using stem-loop RT-PCR, an expression levels change of thirteen miRNA belonging to six miRNA families targeting leaf development and morphogenesis were analyzed upon Tobacco Mosaic Virus (TMV)-tomato infection. Compared to mock plants, significant changes in relative expression levels of nine miRNAs were observed. The miR319c-5p showed the highest statistically significant increase in accumulation at 15 days post-inoculation. At all time points tested, miR159, miR164a-3p, miR164a-5p, miR166c-5p and miR319c-5p were up-regulated while miR160, miR319a, miR319b, miR319c-3p were down-regulated in most cases. Our data could provide new insights into the role of miRNAs in tomato-TMV interaction and in developing efficient strategies for improving tomato resistance against viral infection. | ||
| Keywords | ||
| Host-virus interaction; Stem-loop; RT-PCR; TMV; Viral infection | ||
| References | ||
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