Chanprasartsuk, O., Prakitchaiwattana, Ch., Sanguandeekul, R. (2013). Comparison of Methods for Identification of Yeasts Isolated during Spontaneous Fermentation of Freshly Crushed Pineapple Juices. , 15(7), 1779-1790.
O. Chanprasartsuk; Ch. Prakitchaiwattana; R. Sanguandeekul. "Comparison of Methods for Identification of Yeasts Isolated during Spontaneous Fermentation of Freshly Crushed Pineapple Juices". , 15, 7, 2013, 1779-1790.
Chanprasartsuk, O., Prakitchaiwattana, Ch., Sanguandeekul, R. (2013). 'Comparison of Methods for Identification of Yeasts Isolated during Spontaneous Fermentation of Freshly Crushed Pineapple Juices', , 15(7), pp. 1779-1790.
Chanprasartsuk, O., Prakitchaiwattana, Ch., Sanguandeekul, R. Comparison of Methods for Identification of Yeasts Isolated during Spontaneous Fermentation of Freshly Crushed Pineapple Juices. , 2013; 15(7): 1779-1790.

Comparison of Methods for Identification of Yeasts Isolated during Spontaneous Fermentation of Freshly Crushed Pineapple Juices

Journal of Agricultural Science and Technology
Article 23, Volume 15, Issue 7, 2013, Pages 1779-1790    PDF (996.6 K)
Authors
O. Chanprasartsuk1; Ch. Prakitchaiwattana* 2; R. Sanguandeekul2
1Department of Food Science, Faculty of Science, Burapha University, Bangsaen, Chonburi 20131, Thailand.
2Department of Food Technology, Faculty of Science, Chulalongkorn University, Patumwan, Bangkok 10330, Thailand.
Abstract
The aim of this study was to evaluate the performances of methods such as sequencing of the internal transcribed spacer (ITS) and 26S (D1/D2) regions of ribosomal DNA, RFLP analysis of the ITS region and commercial biochemical test kit for the identification of the yeasts isolated during spontaneous fermentation of fresh crushed pineapple juice. The experiments were conducted in Thailand and Australia. The yeast isolates in Thailand were identified by sequencing the ITS and 26S (D1/D2) regions of ribosomal DNA and RFLP analysis of the ITS region. The yeast isolates in Australia were identified by sequencing analysis of the two DNA regions and commercial biochemical test kit. The identification results conducted in both countries were relatively similar. Mainly, the yeast isolates could be identified by the use of 26S rDNA in combination with ITS sequencing analysis. In Thailand, approximately 80% of the yeast isolates identified by sequencing analysis of the two regions gave similar identities and included Rhodotolula mucilaginosa, Issatchenkia orientalis, Hanseniaspora uvarum, Hanseniaspora opuntiae, Pichia guilliermondii, Aureobasidium pullulans, Saccharomycodes ludwigii, Candida tropicalis, Pichia fermentans, Zygosaccharomyces bailii, Candida stellata and Erythrobasidium hasegawianum.In Australia, 86% of the yeast isolates gave similar identifications by the sequencing analysis of the two regions and included P. guilliermondii, Pichia membranifaciens, P. fermentans, H. uvarum, H. opuntiae, I. orientalis, Candida sp., Yarrowia lipolytica, Tremella globispora, R. mucilaginosa and A. pullulans.
Keywords
Biochemical test kit; Fruit juices; ITS-RFLP; rDNA sequencing; Yeast diversity
References
Statistics
Article View: 130
PDF Download: 111
Statistics
Number of Journals45
Number of Issues2,171
Number of Articles24,674
Article View24,454,668
PDF Download17,557,946