Profiling of Phenolic Compounds, Antioxidant and Cytotoxic Properties of Turkish Black Cumin Seeds | ||
| Journal of Agricultural Science and Technology | ||
| Article 12, Volume 26, Issue 6, 2024, Pages 1333-1345 PDF (8.27 M) | ||
| Document Type: Original Research | ||
| DOI: 10.22034/JAST.26.6.1333 | ||
| Authors | ||
| U. Muazu Yunusa1; R. Ozturk Urek* 2 | ||
| 1Graduate School of Natural and Applied Sciences, Dokuz Eylül University, Izmir, Türkiye. | ||
| 2Department of Chemistry, Division of Biochemistry, Faculty of Science, Dokuz Eylül University, Izmir, Türkiye. | ||
| Abstract | ||
| This research investigated the effects of extraction processes on phenolic compound extractions and evaluated the antioxidant and cytotoxicity properties of Nigella sativa seed. The most abundant phenolic compound, namely, Epigallocatechin Gallate (EgCg), was identified in free flavan-3-ol extract (127.85±4.73 µg g-1 DWE), which exhibited considerable ferrous-ion chelation and cytotoxicity on HepG2 cancer cells. Epigallocatechin (EgC) was the second and recognized in the bound flavan-3-ol extract [(113.31±3.49 µg g-1 DWE (dry weight extract)], which showed the largest 2,2-Diphenyl-1-Picrylhydrazyl Radical (DPPH•) scavenging activity and ferric-ion reducing power, as well as the most cytotoxic on HepG2 (IC50= 24.91±1.45 µg mL-1). Caffeic, sinapic, and p-hydroxybenzoic acids were found in Bound Phenolic acid from Basic-Hydrolysis extract (BPBH), which had the highest Hydroxyl radical (•OH) and Nitric Oxide radical (NO•) scavenging activity and appreciable cytotoxicity on HepG2. Ferulic and p-coumaric acids were detected in Acid-Hydrolysable Phenolic Acid extract (AHPA) and Bound Phenolic acid from Acid-Hydrolysis extract (BPAH), respectively. The two extracts demonstrated higher Ferric-ion Reducing Antioxidant Power (FRAP) values and were also cytotoxic to HeLa and HepG2 cell lines. To the best of our knowledge, EgC, EgCg and rosmarinic acid were identified in N. sativa seed for the first time. Our study indicates N. sativa seeds as a promising source of phenolic compounds with antioxidant and anticancer properties. | ||
| Keywords | ||
| Anticancer properties; Cytotoxicity; Free radicals; Nigella sativa | ||
| References | ||
|
| ||
|
Statistics Article View: 158 PDF Download: 139 |
||
| Number of Journals | 45 |
| Number of Issues | 2,174 |
| Number of Articles | 24,684 |
| Article View | 25,075,774 |
| PDF Download | 17,776,230 |