Natural Deoxynivalenol Contamination of Corn Produced in Golestan and Moqan Areas in Iran | ||
| Journal of Agricultural Science and Technology | ||
| Article 11, Volume 12, Issue 2, 2010, Pages 233-239 PDF (214.24 K) | ||
| Authors | ||
| R. Karami-Osboo1; M. Mirabolfathi* 1; F. Aliakbari2 | ||
| 1Mycotoxins Research Laboratory, Plant Pests and Diseases Research Institute, Tehran, Islamic Republic of Iran. | ||
| 2Islamic Azad University, Tehran, Islamic Republic of Iran. | ||
| Abstract | ||
| Deoxynivalenol contamination was determined in corn produced in Golestan and Ardabil (Moqan) Provinces, Iran, in 2004-2005. Samples were collected from different stages of production, including before harvest, at harvest, post harvest as well as after drying. Ground sub samples were extracted with water; each extracted sample was cleaned up through an immunoaffinity column. Deoxynivalenol was estimated through reversed-phase High Performance Liquid Chromatography (HPLC). The linearity of standard curve for 50 -10,000 ng ml-1 of standard solutions was proved (R2= 0.9999). Detection limit was 10 ng g-1. Recovery of the method for 1,000 and 500 ng g-1 spiked samples was 73.5% and 93.5% (n= 5). Deoxynivalenol contamination was found in 76.7% of samples in the range of 54.4-518.4 ng g-1. The mean of contamination was 116.25 ng g-1. This is the first report of natural DON contamination of corn from Iran. | ||
| Keywords | ||
| Iran; Corn; Deoxynivalenol; HPLC; Natural contamination | ||
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