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There are different methods for the preparation of infusions that can affect the properties of these infusions. The usual method for preparing of infusion is a maceration method that is made using boiling water and a filter. The aim of this study was to prepare marjoram infusion and evaluate the effect of the method of maceration and ultrasonic for different times (5 and 15 min) on phenolic, compounds, free-radical scavenging capacity (DPPH) and heavy metals (arsenic, lead and Cadmium) in the form of a completely randomized design. The results of data analysis obtained from the experiment showed that the preparation method of infusion had a significant effect on all the parameters of the study. The results showed that the amount of phenolic compounds, free-radical scavenging capacity (DPPH) and heavy metals (arsenic, lead and Cadmium)  increased by increasing the time of both processes (ultrasound and maceration). On the other hand, the use of ultrasound waves was found to increase the usefulness of the compounds and also the heavy metals in the marjoram infusion. The total amount of heavy metals measured in this study was less than the standard defined for heavy metals for plant and fruit infusions. Finally, according to the results of this research, it can be stated that using 15 min ultrasound was the best method for making marjoram infusion.
 

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In this study, the effect of enzyme type (alcalase and pepsin) and process time (50-300 minutes) on the degree of hydrolysis and antioxidant indices including free radical scavenging of DPPH, ABTS, hydroxyl, reducing power, and chelating activity of Iron and copper ions were evaluated for navy bean protein (Phaseolus vulgari L.). Also, the composition of amino acids (hydrophobic and antioxidants types) and structural properties (FTIR) of primary protein and hydrolysates were investigated. The results showed that enzymatic hydrolysis improves antioxidant properties. Also, the composition of amino acids has a significant effect on antioxidant activities. On the other hand, the type of enzyme and the time of the hydrolysis process affected the degree of hydrolysis and the antioxidant activity of the hydrolysates. Thus, the highest percentage of free radicals scavenging of DPPH (82.4%), ABTS (58.3%), reducing power (0.97), hydroxyl radical scavenging (57.5%), and chelation of Fe (53.7%) and Cu ions (12.1%) were affected by the type of enzyme and process time. Among different treatments, the highest value of these indices (except copper ion chelating) was related to hydrolysates with alcalase. Structural properties of white bean protein were evaluated and enzymatic hydrolysis caused changes in the amide regions (I and II) as well as exposure to some hydrophobic-buried groups. The results of this study indicated the positive effect of enzymatic hydrolysis on the production of antioxidant hydrolysates that can be used in the food industry.