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Showing 2 results for Vlasenko
Volume 25, Issue 2 (2-2023)
Abstract
Currently, nanotechnologies are being actively introduced into agriculture, in particular in the field of creating new effective plant protection products. This is achieved through the development of nanosized controlled release systems, such as polymer nanoparticles, micelles, and so on using a wide variety of materials. In the present study, we applied original approach based on “green” mechanochemical technology to prepare new nanocomposites of pesticide Tebuconazole (TBC) for treating wheat seeds against pathogenic microflora (B. sorokiniana, Fusarium spp., Alternaria spp., Penicillum spp.). The size distribution of nanoparticles for three TBC formulations (microcapsules, microemulsionsб nanosuspensions) was measured using dynamic light scattering technique. All formulations contained nanoparticles (10-300 nm) and we aimed to find the most suitable size for effective penetration into cell membranes. The narrowest size distribution (225±40 nm) was observed for nanosuspension based on Licorice Extract (LE). The microcapsules based on Na-CMC also contained micro-sized particles (1,500 nm), which are apparently aggregates of nanoparticles. The laboratory and field biological tests revealed a high activity of the developed formulations against all pathogenic microflora under study, with a low retardant effect. Nanosuspension is considered as the most “environmentally friendly preparation”, since it contains only natural LE as an adjunct. This formulation with a consumption rate of 0.25 Lt-1 suppressed 100% B. sorokiniana, Fusarium spp. and Penicillum spp. infections, possibly due to the presence of natural saponin glycyrrhizic acid, which interacts with plant membranes and promotes better penetration of TBC into the grain.
Volume 27, Issue 1 (12-2025)
Abstract
In our previous studies, we prepared by mechanical treatment and tested several formulations of plant protection products based on Tebuconazole (TBC) with different delivery systems. As a result of those studies, polysaccharides showed high efficiency in increasing the solubility and the effectiveness of products based on these polymers. An important task in developing an effective seed treatment is to increase the efficiency of adhesion and penetration. However, the question arises as to which factor is more important for plant protection or which factor plays the main role in the activity of the protectants: the amount of dressing agent on the surface of the seed or the amount penetrated into the grain? This question remained unanswered in previous experiments and the purpose of this study was to find an answer to this question. For this purpose, protectants of various compositions based on TBС and polysaccharides were prepared and spring wheat seeds were treated with them. At the same time, these seeds were divided into two variants: seeds treated with protectant (TBС on the surface that managed to penetrate inside the seeds) and seeds whose surface was washed by water and, so, seeds were without sorbed Tebuconazole (TBС only inside the seeds). Thus, seeds were prepared that contained only the Adsorbed tebuconazole (SPrA) and seeds containing the drug that Penetrated into the grain (SPrP). These two types of seeds were used in biological tests and the results obtained were compared. An analysis of seed germination and infection with the main pathogens showed that SPrA seeds had an advantage over SPrP seeds both in germination and in protection from diseases. Further research in this direction will help to understand the effect of the penetration of drugs into plants on the possibility of increasing their efficiency and yield of grain crops.
