Showing 18 results for Vaziri
Volume 1, Issue 3 (10-2014)
Abstract
Background: The kiss peptins and its receptor G protein coupled receptor (GPR54) or KISS1 receptor system are being described as key signaling molecules for reproductive function in animal models and humans. They play essential roles in regulation of the hypothalamic- pituitary- gonadal (HPG) axis and the onset of puberty and fertility. Objective: This study was performed to delineate the association of T305C (Leu 102 Pro) KISS1 receptor gene mutation with idiopathic female infertility in Iranian women. Methods: In this study, 140 healthy women with at least one child and no history of infertility and abortion and 130 idiopathic infertile women were recruited for this study. By using allele specific PCR (AS-PCR) method, the allele and genotype frequencies among infertile and healthy women were determined. Results: The gene frequencies of the 305 T and C allele of the KISS1 receptor were 45% and 54% among infertile women and 50% and 50% among healthy controls, respectively. The distribution of genotype frequencies in the patients and controls was as follows: TT (Leu/Leu) was 15% and 0%, TC (Leu/Proline) was 60% and 100% and CC (Pro/Pro) was 24% and 0% respectively. Structural analysis was performed using the MedCalc program (version 12). Our results suggests that significant association were not observed in genotype (P=0.8) and allelic (P=0.6) distribution between cases and controls. Conclusions: The data presented show that mutant allele C is not a risk factor for infertility, suggesting that the presence of KISS1 receptor T305C mutation is probably not associated with idiopathic female infertility in this population (P>0.05).
Volume 2, Issue 1 (Spring 2018)
Abstract
Aim: Most scientists are trying to treat cancer, and in this regard were produced numerous anticancer drugs, that adverse effects on non-target tissue. To overcome this, drugs freight to magnetic nanoparticles Chitosan and its carboxymethyl secondary coumpands are biopolymers that are non-toxic, biodegradable therefore found applications in biomedical field. We explain here that glycerol monooleate covered magnetic nanoparticles (GMO-MNPs) capable of transporting hydrophobic anticancer drugs. Method: In the present study, we have expanded 5-fluorouracil (5-FU) that loaded on chitosan MNPs for targeted cancer therapy. Results: The modified nano-adsorbent was then characterized by Fourier Infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), elemental analysis of CHN (9) and thermal weighing analysis (TGA). Lab conditions such as pH, contact time were optimized. To analyze the structure of the sample, X-ray diffraction spectroscopy was used to investigate the magnetic properties of the nanosized particles synthesized by the magnetometer and to detect the phase type formed on the monolayer glycerol matrix network using a polarizing light microscope. Also, the study showed essential oil release in the external environment of 90% for 30 hours. Conclusion: The optimized magnetic nanoparticles according to SEM image, exhibited segregated nanoparticles with sub-spherical smooth morphology and also the high thermal stability of 5-Fluorouracil nanoparticles which indicated a well-established structure of nanoparticles.
Volume 5, Issue 2 (Summer 2021)
Abstract
The rise of bacterial infections has become a serious problem in human societies. As a result, the development of nanocomposite materials based on biocompatible and non-hazardous materials, besides having antimicrobial and biocompatibility or non-cytotoxicity, associated with unique structural properties, possesses a great importance. Research approach: In this study, bacterial cellulose (BC)/polypyrrole (PPy) and zinc nanoparticles (ZnO), which simultaneously have antimicrobial properties and cell proliferation, were introduced as a new generation of nanocomposite scaffolds produced by freeze-drying. To begin with, ZnO with different weight percentages of 1%, 3% and 5% was added to BC and then PPy in the amount of 2 mmol was embedded in the structure by in situ polymerization. FESEM images proved that the nanofibrous and porous structure of BC was also preserved in the presence of PPy and ZnO. However, after adding PPy and ZnO, they formed a dense structure and microstructure of grape clusters. By adding 2 mmol PPy into BC and upon in situ synthesizing, the tensile strength and Young modulus of BC were significantly reduced to 71 MPa and 2.5 GPa, respectively. On the other hand, with the addition of ZnO nanoparticles, the mechanical properties significantly increased (both of Young modulus and tensile strength compared to BC/PPy samples) due to the compaction of the nanocomposite aerogel’s structure and the formation of the interface of ZnO nanoparticles with both polymers of BC and PPy. The observation of the inhibition zone in the culture medium containing two gram-positive and negative bacteria, well proved the antibacterial ability of ternary nanocomposite scaffolds. The results of MT9 related to L929 on aerogels showed that by adding 3% of ZnO nanoparticles, adhesion and cell proliferation increased significantly during different days of 1 day, 5 days and 7 days of culture.
Volume 8, Issue 2 (Spring 2020)
Abstract
Aims: In the present study, random forest (RF) and support vector machine (SVM) were used to assess the applicability of ensemble modeling in landslide susceptibility assessment across the Kolijan Rostaq Watershed in Mazandaran Province, Iran.
Materials & Methods: Both models were used in two modeling modes: 1) A solitary use (i.e., SVM and RF) and 2) Their ensemble with a bivariate statistical model named the weights of evidence (WofE) which then generated two more models, namely SVM-WofE and RF-WofE. Further, the resulting maps of each stage were dually coupled using the weighted arithmetic mean operation and an intermodal blending of the previous stages.
Findings: Accuracy of the models was assessed via the receiver operating characteristic (ROC) curves based on which the goodness-of-fit of the SVM and the SVM-WofE models were 0.817 and 0.841, respectively, while their respective prediction accuracy values were found to be 0.848 and 0.825. The goodness-of-fit of the RF and the RF-WofE models respectively was 0.9 and 0.823, while their respective prediction accuracy values were found to be 0.886 and 0.823. The goodness-of-fit and prediction power of SVM and SVM-WofE ensemble were respectively 0.859 and 0.873. The same increasing pattern was evident for the ensemble of RF and RF-WofE where their goodness-of-fit and prediction power increased, respectively, up to 0.928 and 0.873. Moreover, the goodness-of-fit and prediction power of RF-SVM ensemble were increased up to 0.932 and 0.899, respectively. The results of the averaged Kappa values throughout a 10-fold cross-validation test as an auxiliary accuracy assessment attested to the same results obtained from the ROC curves.
Conclusion: Successive intermodal ensembling approach is a simple and self-explanatory method so far as the context of many data mining techniques with a highly complex structure has been simply benefitted from the weighted averaging technique.
Volume 12, Issue 3 (Summer 2024)
Abstract
Aims: The present research was conducted to compare the effect of cognitive-behavioral therapy and mindfulness on self-care in depressed women with gestational diabetes.
Materials & Methods: This semi-experimental research was conducted on depressed women with gestational diabetes who were referred to the healthcare centers in Ray County, Tehran. Forty-five individuals were selected through convenience sampling and randomly assigned to three groups, including cognitive-behavioral therapy, mindfulness, and control. The two intervention groups, cognitive therapy and mindfulness, received eight sessions of 60 minutes each. The research tools included the Summary of Diabetes Self-Care Activities Scale and the Edinburgh Postnatal Depression Scale. Data analysis was performed using SPSS 25 software and the mixed analysis of variance.
Findings: Mindfulness was significantly more effective in self-care behavior (p<0.01). However, both cognitive-behavioral therapy and mindfulness methods led to increased diabetes self-care activities (p<0.01). After a one-month follow-up, both methods remained effective (p>0.05), although mindfulness showed a decreasing trend (p<0.01).
Conclusion: While both mindfulness and CBT show promise in enhancing self-care among depressed women with gestational diabetes, mindfulness appears particularly effective in addressing the complex interplay of depression and diabetes management. However, CBT demonstrates the potential for longer-lasting therapeutic effects.
Volume 15, Issue 13 (Special Issue 2016)
Abstract
Volume 15, Issue 13 (Special Issue 2016)
Abstract
Volume 16, Issue 1 (8-2013)
Abstract
Objective: Development of high producing mammalian cell lines is a major bottleneck in manufacturing of recombinant therapeutic proteins. This study examines the effect of using the matrix attachment region from the human interferon beta gene in combination with promoter activation strategy with E1A 13S protein on human tissue plasminogen activator (t-PA) expression in Chinese hamster ovary (CHO) cells. Methods: The matrix attachment region was cloned in 3΄ and 5΄ flanking sides of the t-PA expression cassette in pTPA vector to generate pMTPA. After transfection of the cells with pTPA and pMTPA vectors, stable cell pools were developed and the t-PA expression level determined for each stable cell line. In the next step, E1A 13S expression plasmid was transfected to stable cell pools and t-PA titers were measured after 72 hours. Results: Integration of pTPA and pMTPA vectors in the CHO genome was confirmed by PCR analysis on genomic DNA of stable cell pools. Analysis of the t-PA expression level showed a three-fold enhancement in pMTPA transfected cells compared to pTPA-containing cells. t-PA expression was further enhanced up to 1771 U/ml by transient expression of E1A 13S in pMTPA stable cell pools. Conclusion: These results have shown that incorporation of matrix attachment region in an expression vector in combination with promoter activation can effectively enhance recombinant protein expression levels in CHO cells.
Volume 16, Issue 3 (10-2016)
Abstract
Counting the minimum number of differential active S-boxes is a common way to evaluate the security of block ciphers against differential and linear cryptanalysis. In this paper, we use mixed-integer linear programming (MILP)
to calculate minimum number of active S-boxes of the some Feistel structures. We focus on Type-II of Feistel structures
with four and six partitions and explain how to analyze them by MILP when they have more than one MDS2 matrices (like
Clefia) in their structure. Moreover, we propose a new four partitions Feistel structure with three multiple MDS matrices
which have more active S-boxes rather than Clefia structure. We also generalize Clefia structure in to six partitions Feistel
structure by three multiple MDS matrices for 192 bits block size.
Volume 16, Issue 4 (2-2014)
Abstract
Objective: The study of physiological changes in recombinant cell lines provides useful information to improve production performance. In this study, we investigate the effects of an anti-CD33 chimeric IgG4 expression on Sp2.0 cell growth.
Methods: Variable region genes of light and heavy chains of monoclonal antibody produced by M195 were cloned in pFUSE-CLIg-hk and pFUSE-CHIg-hG4 expression vectors, respectively. Transfection of recombinant plasmids into Sp2.0 cell lines was performed using lipofectamine in two steps. Positive transformant cells were isolated and subjected to PCR, RT-PCR and Western blot analysis to confirm the integration of gene cassettes and the expression of recombinant IgG4.
To assess the growth parameters, recombinant and parent Sp2.0 cell lines were seeded at a density of 1×105 cells/ml in duplicate into 12-well plates. For nine days, culture plates were sampled daily and viable cell count and viability determined.
Results: The results of PCR, RT-PCR and Western blot analyses confirmed the generation of stable producer cell lines. In recombinant cells, the maximum cell density decreased by 46%. However, it was observed that IgG4 expression had no effect on cell viability of these transfectants.
Conclusion: Our results showed that the expression of recombinant IgG4 can change growth parameters in Sp2.0 cell lines that express the pFUSE-CHIg-hG4-pFUSE-CLIg-hk construct.
Volume 16, Issue 13 (Conference Special Issue 2017)
Abstract
Marziyeh Khormaeepour, Amir Vazirizadeh, Ghulam Hussain Mohebbi,
Volume 16, Issue 88 (6-2019)
Abstract
Cake is one of the popular, high-consumption, and varied baking industry, that in addition to its nutritional value, has a high consumption in different classes of society. In this research, Stevia was first substituted at 25, 50, 75 percent of sugar, and the characteristics of flavor, texture and color of the product were evaluated and the results showed that the best replacement level was 25%, which was more similar to control.
After selecting the Optimum level of stevia replacement, according to similar previous studies on lemon, 5 and 10% lemon powder levels were selected and added to the product and physicochemical properties were examined. The results indicated that all of the color parameters were reduced by adding lemon peel powder and the cooking loss was lower in the treatment containing 10% lemon peel powder.
Also, the addition of lemon peel powder increased the stiffness and stiffness and reduced the fringe in the product. Adding lemon peel powder significantly reduced the amount of peroxide in comparison with control. Sensory evaluation results showed 5% treatment was more similar to control. In general, it can be concluded that lemon peel and stevia can be used as a functional composition in cake.
Keywords: Dietary fiber, Stevia, Citrus peel, Phenolic compounds, Cakes
Fatemeh Noori Hosseinabadi, Shadab Shahsavari, Ali Vaziri Yazdi, Azadeh Hemmati,
Volume 16, Issue 88 (6-2019)
Abstract
Nowadays the use of natural and biodegradable nanofibers in the packaging industry due to the contamination of non-biodegradable polymers in food packaging is dramatically obvious and electrospinning is one of the easiest ways to produce these nanofibers. In this study, the electrospinning of collagen polymer type I (extracted from the rat-tail) with Beta Cyclodextrin and Nanoclay was investigated and Acetic acid was used as a safe solvent in terms of the environment. After designing the experiments using an experimental design software (Design Expert 7.0), the effects of independent variables such as weight-weight ratio of Beta Cyclodextrin to Collagen (X1), Volume-weight ratio of Nanoclay to Collagen (X2) and solution feed rate (X3) was evaluated on dependent variable, including nanofibers diameter (Y1). Also, electrospinning process was performed with a voltage of 12 Kv and the distance between the needle and the collector 120 mm at ambient temperature and pressure. Nanoclay have been used due to barrier and antimicrobial properties; in addition, Beta cyclodextrin was used for the specificity of the structure that causes hydrophilic and hydrophobic surfaces. Furthermore, to investigate the shape of nanofibers Scanning Electron Microscopy, to investigate the structure Transform Infrared Spectroscopy, to investigate existing elements X-Ray Fluorescence Spectroscopy and to determine thermal resistance Differential scanning calorimetry was applied. The results showed that optimal nanofiber with a average size of 123.01 nm and a flawless structure with a viscosity of 145.33 mpa.s was obtained.
Ali Vaziri, Ali Akbar Safekordi, Azade Shekarabi, Sadaf Shamlou,
Volume 16, Issue 91 (September 2019)
Abstract
Abstract
In recent years, edible coatings have been extensively used for extension shelf life of fruits and vegetables. In this research, the effect of washing with citric acid (1%w/v) followed by coating with different concentrations of carboxymethyl cellulose (CMC;1, 1.5 and 2% (w/v)) in combination with ascorbic acid (AA; 1, 2 and 3% (w/v)) on postharvest quality of button mushrooms when stored at 4 for up to 15 days was studied. After treatment, mushrooms weight loss, soluble solid content, firmness, color, microbial and sensory quality were evaluated at 1, 4, 8, 12 and 15 days of storage. The result indicated that coating reduced weight loss and delayed changes of soluble solid content and color compared to uncoated control mushroom. Coatings had not remarkable effect on firmness of mushrooms. Sensory evaluation showed that the shelf life of mushrooms increased from 8 to 15 days. Washing with citric acid reduced total bacteria, yeast and mold counts. Among different coatings the combination of CMC 2% and AA 3% was more effective.
Volume 17, Issue 3 (5-2017)
Abstract
At the core of the conventional hydro cyclone, the secondary flow is created in opposite direction of the primary flow which is adjacent the wall and causes a sink pressure on the central axis of the hydro cyclone. This low pressure zone may drag fine particles to the core and escape them from the upper section of hydro cyclone. In this research by adding a sound source at the core of the hydro cyclone, the separation performance of an acoustic hydro cyclone is studied. Then the effects of the hydro cyclone’s body tilt angle with the horizon reviewed in next step. To this end, the flow simulation carried out by the appropriate turbulent and two-phase fluid flow model selection and consequently the results validated with experimental data. The result shows the optimum strength and frequency of acoustic stimulation and the performance of conventional hydro cyclone in different tilt angles, particles diameter and inlet velocities. In addition in this study, the optimum injection velocity for any diameters which use of sound source impacts greatly on increasing the separation efficiency, is introduced. Finally, by applying a genetic algorithm with two objectives function, among all the states, the model that have the highest efficiency and lowest pressure drop is selected.
Volume 17, Issue 8 (10-2017)
Abstract
One of the recent techniques which has attracted attention from researchers is the use of Nano particles to reinforce composites. While the technique does not make any changes in the weight of the structure, it improves its mechanical and physical properties. One form of Nano particles includes Carbonic Nano Tubes (CNT). Since its discovery, CNT has found wide application in industryIn this article, the response of aluminum hybrid panels and composites made from epoxy-Kevlar and aluminum hybrid panels and Nano-composites made from epoxy-Kevlar to ballistic impact was studied. Four groups of the panels were constructed using 0, 0.5, 1 and 1.5 percent of carbon nanotubes (CNT)s. The hybrid samples constructed and tried out in this experiment has been done using manual layer-making and heated press.The thickness of the panels constructed from two aluminum plates and ten Kevlar 29 plates was consistent. The ballistic impact test using gas gun was implementated by conical bullet 7.6 gr shooting in two avrege velocities (220 m/s and 275 m/s). input and output velocity with each sample was assessed using a laser speedometer. The amount of energy absorption and special energy absorption of passing of the rocket was determined as the criteria for the comparison of the efficiency of ballistic of different panels. The results indicated that among the four samples examined, the panel made up of 1 percent CNTs had the most amount of energy absorption and ballistic resistance.
Shadi Ostowar, Moharam Vaziri, Fardin Tamjidi,
Volume 19, Issue 126 (August 2022)
Abstract
In this study, the effect of different levels of inulin (0 to 5%) and egg white powder (0 to 10%) on protein content and sensory characteristics of taste, aroma, softness, spreadability and overall acceptability of spreadable processed cheese was investigated. The results were modeled and analyzed by the response surface methodology (RSM) based on a central composite design (CCD). For all response variables, the R2 values of the models ranged from 0.86 to 0.99, and the values of lack-of-fit factors were not significant (P >0.05); Therefore, the accuracy of the models for fitting the data was confirmed. In general, inulin decreased, but egg white powder increased the protein percentage of processed cheese. Inulin had a favorable effect on the softness and spreadability of processed cheese, but reduced the scores of taste, aroma and overall acceptability, especially at high concentration (5%). In contrast, egg white powder improved the taste, aroma, and overall acceptability of processed cheese, but reduced its softness and had little effect on its spreadability. The combination of inulin and egg white powder moderated each other's negative effects on the sensory properties of the fortified processed cheese, and ultimately improved its sensory properties compared to the control sample.
Pari Sohrabi, Moharam Vaziri, Fardin Tamjidi,
Volume 20, Issue 138 (August 2023)
Abstract
In this research, the effect of storage time on the characteristics of extensibility and consistency of processed cheese samples produced from egg white, corn oil and soy protein was investigated. In this research, firstly, in order to optimize the formulation of processed cheese from the central composite statistical design in three levels for three independent variables including soy protein isolate (0, 7.5, 15%W/V), corn oil (0, 8, 16%V/V) and egg white. Egg (0, 3, 6%V/V) was used. On the other hand, the optimal formulation of processed cheese was obtained with the objective of maximum scores of the two characteristics of extensibility and texture continuity, using the utility function method. Finally, the effect of storage in three time periods (0, 1, and 2 months) on the two characteristics of extensibility and consistency of the optimized sample texture was investigated by relying on physical changes. According to the obtained results, the effect of time on the extensibility characteristic showed that with the passage of time, the extensibility of processed cheese samples increased significantly in the negative direction. According to the results, the interaction effect of egg white/time and corn oil/time; It showed that with the passage of time (in the presence and absence of egg white and corn oil), the expandability of the produced samples increased significantly in the negative direction. Also, the effect of time on consistency properties showed that with the passage of time, the consistency of produced processed cheese samples decreased significantly. The results of this study are favorable for the development of processed cheese production and profitable dairy products containing egg white, corn oil, and soy protein. .
