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Our understanding of the reality of our knowledge and understanding of changing the way we know to be changed. To understand reality in faced with the complex realities in spiritual terms; we are forced to adopt methods that their main claim to discover hidden meanings and realize it is a fact. Religious and mystical experience in understanding the phenomena are very spiritual and sacred mixed, reduced to social or psychological reality, they can understand and prevent the use of empirical phenomenology to understand religious experience makes it inevitable. This article has attempted to offer gains over the experimental phenomenology and methodological principles of the present one, demonstrate the conceptual complexity of religious experience on the other hand, inevitably use this method to show understanding of religious experience And with providing a real example of the spiritual experience of pilgrimage in Iran empirical phenomenology emerges as an example for understanding the religious and mystical experiences are introduced.        

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 Beet curly top Iran virus (BCTIV) and Beet curly top virus (BCTV) are responsible for the curly top disease in sugar beet Beta vulgaris L. and many other plants. Mixed infection by BCTIV and BCTV in sugar beet plants results in a synergistic interaction, with more severe symptoms than plants infected by either virus, accompanied by an increase in BCTIV and a decrease in BCTV titers. Interaction of the Replication associated protein (Rep) with the nonanucleotide motif within the origin of replication is crucial for the replication of the geminivirus genome. Using an in silico approach, we investigated the possible contribution of the interaction between Rep and the nonanucleotide motifs in the interference between BCTIV and BCTV in mixed infections. The physicochemical characterization of both Reps was performed, and their secondary and tertiary structures were predicted by SOMPA tool and I-TASSER server, respectively. Then, the binding affinity of each Rep towards cognate and non-cognate viral nonanucleotide motifs was assessed using Docking simulations. Cluster analysis of HADDOCK revealed that the total binding energy of BCTV Rep toward its cognate nonanucleotide motif was lower than for the BCTIV complex, confirming a higher affinity of BCTV encoded Rep for its nonanucleotide motif. Interestingly, the BCTIV Rep showed the highest affinity for the nonanucleotide motif of the non-cognate BCTV nonanucleotide motif. Since the replication of geminiviruses relies on species-specific Rep interactions and activities, this result could be considered responsible for the competitive interference of BCTIV towards BCTV.

 

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Hibiscus chlorotic ringspot virus (HCRSV), genus Betacarmovirus, family Tombusviridae, is a common pathogen of hibiscus plants in tropical and subtropical regions. During 2020-2021, leaf samples of Chinese hibiscus Hibiscus rosa-sinensis L. with mottling and chlorotic ring spot symptoms were collected from Ahvaz and Molasani Khuzestan province, southwestern Iran. Total RNA extracted from symptomatic samples was subjected to RT-PCR analysis to amplify the sequence of the coat protein gene (CP) (p38) of HCRSV. Complete (1038 bp) and partial (932 bp) p38 sequences were determined and deposited in the GenBank database. The consensus sequences obtained from CP were compared with those of known isolates using the nBLAST program and phylogenetic analysis. The phylogenetic tree constructed based on the p38 sequences showed different ancestors for Iranian isolates of HCRSV. Additionally, the isolates studied were grouped into clades regardless of their geographic distribution, suggesting that there is no differentiation of population based on location and that populations are interconnected. Recombination analysis based on p38 sequences predicted at least two acceptable recombinant isolates, Ahvaz (Iran) and Israel. In silico prediction of CP structures of isolates involved in recombination events showed low sequence to structure identity between HCRSV isolates. In addition to reporting two new HCRSV isolates from Iran, our work demonstrated that HCRSV exhibits a high genetic variation through recombination and that the classification criterion could be changed from low nucleotide sequence identity to a higher value, along with the structural analysis of betacarmovirus proteins.