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Tomato waste can be used as a new protein source for the production of hydrolyzed protein. Free radicals are among the most important factors in the development of cancer and genetic mutations, which have become one of the greatest threats for human health in recent centuries. In this research, hydrolysis condition of tomato seed protein (temperature, time and amount of alcalase enzyme) to achieve maximum antioxidant activity and nitric oxide reducing power were investigated. The values were evaluated using Design Expert software and analyzed by the response surface methodology. Results showed that with the optimimizing hydrolysis conditions using the alcalase enzyme, it is possible to achieve a products with significant DPPH radical scavenging activity and nitric oxide reducing power. The results indicate that optimum conditions for achieving the maximum DPPH free radical inhibition activity and nitric oxide reducing power is temperature of 50 ° C, hydrolysis time 210 min, and the amount of enzyme 1.85%. Under these condition the amount of DPPH free radical inhibition activity and nitric oxide reducing power was 85.53 and 61.17, respectively. The results showed that the hydrolyzed protein produced from tomato seed could be used as a natural additive in the foods formulation and pharmaceutical uses with high antioxidants and nitric oxide reducing activity.

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The waste from food processing involves high-quality nutrients that can be widely used in food formulations. About 8100 tons of tomato pomace is produced annually by factories, which are often discarded without taking into account their potential use in the supply chain. Seeds isolated from tomato pomace are rich in protein and essential amino acids. In this research, chemical composition (protein, fat, moisture and ash) and functional properties (water absorption, oil absorption, foaming property and emulsion activity), in different fractions of tomato seed (defatted meal, protein concentrate and hydrolysate) and soy isolate (as the control sample) was determined and the effect of different concentrations of salt and pH on these characteristics was also investigated. The results showed that the tomato protein hydrolysate contained 79.37% of protein. There was a significant difference between the samples in terms of functional characteristics (water absorption, oil absorption, foaming and emulsification properties) ​​between the de-oiled and concentrate samples (P <0.05). In conclusion, it can be said that the protein hydrolysate derived from tomato seeds protein showed the best functional properties and therefore could be used as substitute for animal based proteins in the diet as well as active ingredients in food formulations.

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About 8100 tons of tomato pulp is produced annually by factories, which in many cases are eliminated from the consumption chain, regardless of their uses. Tomato waste can be used as a new protein source for the production of hydrolyzad protein. Therefore, in this study, by adding the tomato seed protein hydrolyzate to sausage formulation as a functional ingredient, its effect on physicochemical properties including residual nitrite, color, and sausage texture during storage period was investigated. The results showed that the samples containing hydrolyzed protein had less residual nitrite than the control sample, and over time, the residual nitrite content was reduced in all samples. Also, the brightness and redness indicators of the product increased over time. The results of this study showed that the tomato seed protein hydrolyzate with good nitrite inhibition activity can be used as a functional ingredient