Showing 3 results for Shafighi
Volume 2, Issue 3 ((Articles in Persian) 2011)
Abstract
Comparative literature pays attention to the study of literature confluence literature in various languages and its complex relationship in the past and present as well as its historical relationships in term of effectiveness in the field of arts, literary schools, intellectual current issues and people. From this respect, comparing eastern oriented works with western ones as two opposite poles enjoys a particular importance. The present paper proposes the hypothesis that the play King Lear, a work of Shakespeare, is rooted in Iranian legends. After showing the possible relationship of King Lear with East, We has tried to compare the story of King Lear with several Iranian stories and myths such as Fereydoun myth, Indian GOl Bekavoli, devine story of Zatol Sour cast, story of tried, drunk Mirza and the story of king and his three daughters. By decreasing the King Lear to nine fundamental stories and comparing with them western samples, it was made clear that more than ninety percent of the King Lear stories are similar to the Iranian ones. Researches like this in the field of literature studies in modern Iran are not unprecedented. So as far as the authors know, no independent research on Shakespeare’s King Lear based on the above - mentioned hypothesis has so far been carried out.
Volume 8, Issue 4 (fall 2022)
Abstract
Backgrounds: This study was done to evaluate the distribution of virulence-associated genes and antibiotic resistance in avian colibacillosis-causing Escherichia coli (E. coli) isolates.
Materials & Methods: In this study, 122 E. coli strains isolated from colibacillosis-suspected chickens in commercial broiler poultry farms (Guilan province, Iran) were examined for the presence of 12 virulence genes (hlyF, iroN, iss, iutA, ompT, astA, tra, sfa-foc, papC, fimH, cvi/cva, and Tia) using polymerase chain reaction (PCR). Antimicrobial susceptibility assessment was performed for the isolates using disc diffusion method against 19 antibiotics.
Findings: The fimH, iut, tra, iss, iroN, hly, and ompT genes were detected as the most prevalent genes among colibacillosis-causing isolates (more than 70%), while sfa-foc (S fimbriae and F1C fimbriae subunits) had the lowest frequency among colibacillosis-causing E. coli isolates (3.28%).
Conclusion: Virulence-associated genes were frequently detected in avian pathogenic E. coli strains. These findings could help better understand the pathogenicity potential of E. coli in poultry. Preventative measures are necessary to reduce food and environmental contamination with avian E. coli strains.
Volume 21, Issue 2 (Summer 2018)
Abstract
Aims: Breast cancer is the most common cancer among women around the world and in Iran and 22% of all cancers in women is included.
About 30% of cases with breast cancers are due to the proliferation or excessive expression of HER-2 protein. Chitosan is an environmentally friendly combination, due to its minimal systemic toxicity of peptide or drug delivery, the application is considered. The aims of this study were to manufactur chitosan nanoparticle containing HER-2 recombinant protein, evaluation of the properties and effect of its oral and injection immunization on spleen lymphocytes.
Materials and Methods: In this experimental study, pET-28a vector containing HER-2 gene was evaluated using PCR by universal primers. Expression of protein in E.coli was induced with IPTG. The recombinant protein was purified using affinity chromatography and evaluated by Western Blotting analysis. Chitosan nanoparticles containing recombinant protein were prepared by inonic gelation method and their size were characterized by DLS. Mice were immunized with nanoparticles and antibody titers were determined by ELISA. The response of lymphocytes in exposure to nanoparticles was evaluated by MTT assay. One way ANOVA, Duncan test and SPSS 22 software were used for statistical analysis.
Findings: The protein with a molecular weight of 18 kDa was confirmed. Yield of protein was 12mgL-1. Encapsulation efficiency of recombinant protein in nanoparticles was 70%. The average particle size was 205.2nm. Immunization of mice induced mucosal and humoral immune response.
Conclusion: Encapsulation efficiency of recombinant protein in nanoparticles is 70%. The average particle size is 205.2nm. Immunization of mice induces mucosal and humoral immune response HER-2 recombinant protein, due to the cellular and humoral immune response, is a candidate suitable for cancer diagnostic or therapeutic purposes.
