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The study was conducted to assess the effect of different wax coating materials and Modified Atmosphere Packaging (MAP) on storage life and quality of ‘Kaghzi lime’ (Citrus aurantifolia Swing.) fruits. The fruits were harvested at light yellow skin color stage and treated with different wax coating materials: T₁= Citrus Wax (wood resins18%, Imazalil 0.3%, Thiabendazloe 0.5%), T₂= PHRC SCM Wax [9% total solids (castor and shellac based wax)]; T₃=Modified Atmosphere Packaging (MAP), and T₀= The fruit without any treatment were used as control. The fruits were stored at 10°C for 45 days and relative humidity was maintained at 90%. Physiochemical, sensory, organoleptic, and quality parameters were measured after 30 and 45 days of harvesting. The coating treatments significantly (P≤ 0.05) reduced physiological weight loss, increased shelf life and maintained the quality of fruits. Among all treatments, Citrus Wax proved best because it maintained relatively higher levels of acidity, flavor, vitamin C content and fruit firmness; prevented disease attack; and improved juice recovery of lime fruits compared with control. Thus this treatment can be used to enhance the shelf life and maintain quality of the lime fruits. 

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MicroRNAs (miRNAs) are typically small, endogenous, non-coding RNAs molecules that regulate gene expression at post-transcriptional level by mRNA degradation or translational repression. They are composed of 18-26 nucleotides and are conserved during evolution for the development of new miRNAs in a variety of plants. Sugarcane (Saccharum officinarum) is generally a valuable food and forage crop grown all over the world. Until now, different sugarcane miRNAs have been characterized for plant development and stress responses. In this research, 50 unique conserved sugarcane miRNAs from 44 different miRNA families have been predicted using a variety of genomics-based tools. The predicted sugarcane miRNAs were validated using a set of 15 randomly chosen primers and RT-PCR. Stem loop secondary structures are created using MFOLD tool. The psRNA-Target algorithm identified 7,976 various protein targets of sof-miRNAs including 55 specific GO terms. They have significant targets in biological, cellular, and molecular functions. Moreover, the sof-miR5205a regulates sulfur compound biosynthetic process and 9653a directs ubiquitin-dependent protein catabolic process. Consequently, the RNA binding and thylakoid membrane are controlled by sof-miR9657b and 2091, respectively. As a result, the outcomes of the novel sugarcane miRNAs target a variety of substantial genes that aid in controlling the environment for sugarcane to produce a higher quality crop.