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Showing 2 results for Ranadev
Volume 0, Issue 0 (ARTICLES IN PRESS 2024)
Abstract
This study evaluated the efficacy of entomopathogenic fungi (EPF) as biocontrol agents against aphids, whiteflies and western flower thrips. The research employed a leaf disc bioassay with various conidia concentrations to determine lethal concentration (LC) and time (LT) for pest eradication. Additionally, the study assessed the activity of cuticle-degrading enzymes produced by EPF (Chitinase, Protease, and Lipase) to understand their pathogenic mechanisms. Molecular identification using ITS region of 18S rDNA identified virulent isolates. Results indicated that four isolates, ENPF-16, 24, 41, and 60, achieved significant mortality rates (95% to 100%) at a concentration of 1x108 conidia/mL after nine days. Akanthomyces sp. (ENPF-41) exhibited the highest enzyme activity, followed by Beauveria sp. (ENPF-60). The virulent fungal isolates were identified as Beauveria bassiana and Akanthomyces lecanii. Among EPFs, Akanthomyces lecanii (MT997935) displayed greater virulence against all three test insects with lower LC50 and LT50 values compared to other EPFs. In summary, all fungal isolates induced mortality in the tested pests, but their effectiveness varied. Akanthomyces lecanii (MT997935) emerged as a promising biocontrol candidate due to its broad host range and strong virulence.
Volume 26, Issue 5 (9-2024)
Abstract
Production of high-quality inoculum in sufficient quantities is crucial for biocontrol programs. Entomopathogenic Fungi (EPF) are highly suitable biocontrol agents due to their adaptability, mode of action, persistence, and wide host range. This study aimed to evaluate the suitability of agro-wastes, including sugarcane bagasse, paddy husk, Post Mushroom Substrate (PMS), and sorghum grains with and without 10% molasses fortification, for mass production of four EPF isolates (Cordyceps fumosorosea: MT997932, Beauveria bassiana: MT997933, Akanthomyces lecanii: MT997935, and Hirsutella thompsonii: MT997936) isolated from two agro-climatic zones in Karnataka, India. The study employed solid-state fermentation. Results showed that sorghum grains fortified with 10% molasses had the highest mycelial growth and spore production of all isolates, followed by PMS with 10% molasses (T7). Fortification with molasses positively influenced the growth and spore production of EPF. The results indicated that while sorghum grains were the best choice for mass production, PMS fortified with molasses also had great potential as an alternative substrate.
