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Showing 3 results for Ragolia
Volume 4, Issue 2 (Spring 2018)
Abstract
Aims: Streptococcus mutans (S. mutans) is part of human oral cavity microbiome and is known to be responsible of dental caries. The aim of this study was to evaluate the inhibitory effects of Punica granatum, Ricinus communis, and Allium sativum extracts on biofilm formation caused by S. mutans.
Materials and Methods: In this experimental study, the biofilm formation was carried out by broth dilution method with glucose -supplemented Tryptic Soy Agar (TSB) in 96-well microtiter plates. Seven serial dilutions from the aqueous extracts of the Punica granatum, Ricinus communis, and Allium sativum were prepared. Then, a suspension of S. mutans was added to the wells. The anti-biofilm effects of the extracts and turbidity were measured by an ELISA reader apparatus at OD492nm. Experiments were completed in triplicate.
Findings: Ricinus communis was more active on S. mutans than other extracts. In comparison with others, the mean OD obtained in the presence of a concentration of 50mg of the plant extract (OD=0.083) was close to the negative control (OD=0.068). This plant was effective in higher concentrations (50, 25, 12.5 and 6.25mg/ml). Allium sativum extract has a moderate effect on S. mutans. The lowest activity belonged to Punica granatum extract.
Conclusion: The extract of Ricinus communis has strong anti-biofilm activity against Streptococcus mutans, when compared to other extracts, Allium sativum extract show moderate activity on the biofilm formation. Aqueous extract of Punica granatum peel isn’t very effective on S. mutans.
Volume 4, Issue 3 (Summer 2018)
Abstract
Aims: Helicobacter pylori is a pathogen that can be colonized in the stomach. Most laboratories only use IgG and not IgA antibody to diagnose infection. The aim of this study was to compare both IgG and IgA-antibodies level for the detection H. pylori.
Materials & Methods: The presence of IgG and IgA antibodies in the sera of the 517 patients suspected to H. pylori infection was evaluated by Enzyme-Linked Immunoadsordent Assays (ELISA) method.
Findings: The positive cases of infection on the basis of IgG and IgA titers were 68% and 27%, respectively. Also, 7% of the patients with IgG negative were IgA positive.
Conclusion: The comparison of antibody responses in our patients indicate that the sensitivity of IgA level is lower than IgG ELISA and both antibody titers must be evaluated for the identification of infection. In some cases, patients with IgG negative may have IgA positive assays; therefore, in the serological diagnostic process and without endoscopy, IgG results in association with IgA against H. pylori will be completed.
Volume 5, Issue 2 (Spring 2019)
Abstract
Aim: Certain Mycoplasma species, the smallest and simplest free-living bacteria which lack a rigid cell wall, are considered as important pathogenic organisms in human and recognized to have a role in rheumatoid arthritis. The aim of this study was to use molecular methods to detect Mycoplasma spp. in synovial fluid of patients with reactive arthritis in comparison with patients suffering from non-inflammatory arthritis as a control group.
Materials & Methods: Synovial fluid samples were collected from 99 patients with arthritis, all of which fulfilled the standard criteria of American College of Rheumatology for the diagnosis of inflammatory arthritis (59 patients) or non-inflammatory arthritis (40 patients). The DNA of all synovial fluid samples was extracted, and PCR was performed with a specific set of general primers for 16S rRNA of Mycoplasma genus. The PCR products were confirmed via restriction enzyme digestion using BamH1 and sequencing.
Finding: A total of 11 out of 99 (11.1%) samples of patients with reactive arthritis revealed a 270bp amplification band. Digesting the PCR product of 16S rRNA by BamH1 confirmed the PCR assay. The sequencing also confirmed the amplified products.
Conclusion: The pathophysiology of inflammatory arthritis could be attributed, at least in part, to the persistence of bacterial DNA in the joint of patients with reactive arthritis.
