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In this study, bacterial cellulose based antioxidant films containing 5%wt of rosemary essential oil (REO) were prepared. The effect of lamination (production of multilayer film) and beta-cyclodextrin (β-CD) (as complex agent) on the physicochemical, antioxidant and release properties of the active films was studied. X-ray diffraction analysis revealed that the REO was able to decrease the crystallinity of bacterial cellulose film. Results of physical tests revealed that the addition of 5% REO caused to increase of water vapor permeability and decrease of mechanical stiffness and using of β-CD and lamination diminished these negative effects of REO and effect of lamination was more than β-CD. REO had a significant effect on color properties and caused to increase b values and decrease L values. REO loaded film had good antioxidant activity (68.7%) but this property decreased after lamination and β-CD complexion.  The β-CD and lamination decreased the migration rate of REO from matrix of bacterial cellulose films and the effect of lamination was more than β-CD.