Showing 6 results for Pirestani
Volume 5, Issue 3 (Summer 2019)
Abstract
Aims: Toxoplasmosis is a cosmopolitan zoonotic disease caused by an obligate apicomplexan intracellular parasite known as Toxoplasma gondii (T. gondii). Recently, toxoplasmosis has been suggested as a risk factor for diabetes. Thus, the present study aimed to assess the association between T. gondii infection and two types of diabetes in Tehran, the capital of Iran.
Materials & Methods: In the current cross-sectional study, 98, 95, and 94 blood samples were collected from Type 1 and Type 2 diabetic and nondiabetic individuals, referring to Imam Sajad hospital from February to August 2018, respectively. Anti-T. gondii specific IgG and IgM antibodies were measured using enzyme-linked immunosorbent assay (ELISA). Moreover, a structured demographic questionnaire was completed for each person.
Results: IgG antibody was found to be positive in 16.32 (16 of 98) and 57.89% (55 of 95) of patients with diabetes Type 1 and Type 2 and 17.02% (16 of 94) of nondiabetic individuals as controls, respectively. However, the prevalence of positive IgM antibody in these groups was determined as 2.04 (2 of 98), 6.32 (6 of 95), and 17.02 % (16 of 94), respectively.
Conclusion: This finding revealed that toxoplasmosis could be considered as a possible risk factor for diabetes Type 2, while no statistically significant association was found between T. gondii infection and diabetes Type 1. More research is required to be conducted in the future in order to better understand this association.
Volume 8, Issue 1 (Winter 2022)
Abstract
Backgrounds: Trichomoniasis is one of the most common sexually transmitted infections in the world. The main aim of the present descriptive study was to determine the prevalence rate and clinical symptoms of trichomoniasis among women referring to the hospital in Mahshahr city in Khuzestan province, southwest of Iran.
Materials & Methods: Urine samples were collected from 2200 women referring to Imam Musa Kazim hospital in Mahshahr city. In addition, 500 Pap smear samples were used for early detection of Trichomonas vaginalis. At first, parasitological tests were performed to detect T. vaginalis in urine and dissolved Pap smear samples using microscopic examination. Finally, DNA extraction was performed on 34 parasites isolated from positive urine and Pap smear samples. Then the 18s rRNA gene of the parasite was amplified by PCR method. The PCR products of the 18s rRNA gene were finally sequenced.
Findings: The prevalence rate of this parasite was determined to be 1.54%. The highest prevalence rate of infection and clinical symptoms were observed in women aged 31-40 years. Totally, clinical symptoms were observed in 64.70% of infected women, including vaginal itching and irritation (64.70%) and abnormal discharge (26.47%).
Conclusion: The prevalence rate of Trichomonas infection was relatively low in women living in Mahshahr. In addition, about 35.29% of infected women were found to be clinically asymptomatic.
Volume 12, Issue 3 (Number 3 - 2010)
Abstract
Changes in the fatty acid content, during frozen storage at -24°C of Caspian kutum (Rutilus frisii kutum), golden grey mullet (Liza aurata), common carp (Caprinus carpio), pike perch (Sander lucioperca) and common kilka (Clupeonella cultiventris caspia), caught from south Caspian Sea were studied in the present work. Changes in saturated fatty acids (SFAs), monounsaturated fatty acids (MUFAs), polyunsaturated fatty acids (PUFAs), ecosapentaenoic acid plus docosahexaenoic acid/palmitic acid (EPA+DHA/C16), n3 PUFA/n6 PUFA (n3/n6) and polyunsaturated fatty acids/saturated fatty acids (PUFA/SFA) were investigated during a six month period. Results indicated that due to the decrease in unsaturated fatty acids particularly PUFAs (9.25-23.03%), and lower ratios of n3/n6 (2.02-6.06), EPA+DHA/C16 (polyene index; 0.21-0.65) and PUFA/SFA (0.18-0.64) the poststorage nutritional values of these species significantly decrease.
Volume 14, Issue 3 (9-2011)
Abstract
Objective: Microsporidia are ubiquitous opportunistic pathogens infecting all animal phyla. The purpose of this study was to characterization human-associated microsporidia in pigeons of Tehran by staining and molecular methods.
Materials and Methods: In the year 2010 a total of 147 pigeon’s fecal samples were randomly collected from bird stores and public parks of Tehran and screened for the existence of human pathogenic microsporidia by staining method and multiplex/Nested-PCR and RFLP techniques.
Results: Nineteen (12.92%) of the studied samples were positive by microscopic examination, and 31 (21.08%) isolates were detected with specific primers. Genotyping based on the ITS regions of the rRNA gene were done for the Entrocytozoon bieneusi, Encephalitozoon intestinalis, Enc. hellem and Enc. cuniculi, respectively. The genotypes of Ent. bieneusi were identical to the D, M and J; genotypes of Enc. hellem were similar to the genotype 1 and 3 and genotypes of Enc. cuniculi were equal to I and II genotypes which previously characterized in human and animal origins.
Conclusion: These results revealed that there is no limits to microsporidia transmission between pigeons of Tehran and humans for human infective species. This study points to the hygienic importance of this bird, because feces of pigeons are one of the sources of infection with microsporidia in human and easily pollutes our environment; on the other hand, children and elderly people comprise the principal visitors of public parks and they are the populations at risk for microsporidiosis.
Volume 16, Issue 4 (2-2014)
Abstract
Objective: Trichomonas species usually reside in the mouth and occasionally in the respiratory tract. These species can be found in the lungs of humans. Although the pathogenicity of the parasite in the respiratory system has not been proven, it is more prevalent in people who lack good oral health or suffer from asthma or chronic pulmonary diseases. In the present descriptive study, we have identified Trichomonas by direct microscopic observation of stained smears and by PCR on the lung sputum of patients with asthma and chronic lung diseases that include lung cancer, bronchiectasis, COPD, and malignant pulmonary disease who were admitted to MasihDaneshvariHospital, Tehran, Iran.
Methods: For direct examination of 133 sputum samples, we stained the smears with giemsa. In addition a total of 60 samples were used for DNA extraction by an extraction kit (Cinnagen). Nested-PCR was used for amplifying the ITS1 target gene of the parasite. Finally the DNA sequence of the gene was determined.
Results: According to the results, in direct examination of the sputum smears there were only 4 positive cases identified, whereas 22 (36.66%) of the samples were identified as Trichomonasby nested PCR. According to gender, 33.33% of the female samples and 38.46% of the male samples were found to be positive.
Conclusion: Considering the high prevalence of this parasite in the study group, chronic pulmonary disease and asthmatic patients may be more susceptible to Trichomonas.
Volume 21, Issue 3 (Fall 2018)
Abstract
Aims: Giardia and Cryptosporidium are enteric protozoans, causing gastrointestinal infection in humans and animals such as mammalian and birds worldwide. Both protozoans causae diarrhea and nutritional disorders in human. The present research aimed at investigating the Giardia and Cryptosporidium infection and genotyping common Giardia in children in Alborz province.
Materials and Methods: In this descriptive cross sectional study, 160 stool samples of children aged 2-10 years old in Alborz province were first stained with the modified Ziehl-Neelsen staining method for Cryptosporidium and trichrome staining method for Giardia, and they were evaluated for infection with these two protozoans. Then, positive samples were analyzed by molecular method to genotype. Giardia duodenalis based on the TPI gene and Cryptosporidium based on the 18SrRNA gene were investigated by Nested PCR and to determine the groups and subgroups by sequencing. For drawing phylogenetic tree, MEGA 7 software and Maximum liklihood algorithm with 1000 replications were used.
Findings: Cryptosporidium was detected in 4 (2.50%) samples, while Giardia was detected in 15 (9.37 %) samples with staining method. In no sample of patients with clinical symptoms of diarrhea, Cryptosporidium was observed. Among 4 isolates of Giardia duodenalis, 1 BIV, 2 BIII, and 1 All isolates were detected, some of which belonged to asymptomatic children and the drawn phylogenetic tree confirmed it. Attempts to determine genotype Cryptosporidium failed.
Conclusion: Identification of AII, BIV, and BIII genotypes in 2-10 years old children in Alborz province indicates anthroponotic and anthropozoonotic transmission of Giardia infection, respectively, in this province.
