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Showing 4 results for Owlia
Epidemiology of Human Papillomavirus (HPV) Infection among Iranian Women Identified with Cervical Infections: A Systematic Review and Meta-Analysis of National Data

Volume 3, Issue 2 (5-2017)
Abstract
Background: Human papillomavirus (HPV) is one of the most common causes of sexually transmitted disease (STD) in humans. HPV is associated with gynecologic malignancy and cervical cancer among women worldwide. In the current study we sought to determine the prevalence rate of HPV in Iranian women identified with cervical infections.
Materials and Methods:  Prevalence rate of HPV in Iran was investigated from 2000-2016 using several databases including Medline, Web of Science, Embase, Google Scholar, Iranmedex, and Scientific Information Database. Statistical analysis was performed by Comprehensive Meta-Analysis (V2.2, Biostat) software. Random effects models were used by taking into account the possibility of the heterogeneity between the studies, which was tested through the Cochran’s Q-statistic.
Results: The meta-analyses showed that the prevalence rate of HPV infections was 38.6 % (95% CI, 27.9-50.5) among Iranian women with cervical infections. The further stratified analyses indicated that the prevalence rate of HPV was higher in the studies conducted during the 2000-2008 years.
Conclusion: The results of the present study underscore the need for further enforcement of STD control strategies in Iran. Establishing advanced diagnostic facilities for HPV, vaccination of high risk groups, and continuous monitoring of HPV are recommended for HPV prevention and control.
Polymorphism of Virulence Factor Genes Prn and Fim of Bordetella Pertussis Clinical Isolates in Iran

Volume 5, Issue 2 (Spring 2019)
Abstract
Objectives: Bordetella pertussis is a Gram-negative coccobacillus bacterium which is the causative agent of whooping cough. In recent years, the number of whooping cough cases has been rised. This bacterium has important virulence factors such as fimbriae and pertactin. In this study, polymorphism of Serotype 2 and 3 fimbriae genes and 2 Regions of pertactin gene were surveyed.
Materials & Methods: Totally, 20 B. pertussis clinical isolates were tested. DNA was extracted using the kit. Serotypes 2 and 3 fimbriae genes and pertactin Region 1 and 2 were identified using PCR method; finally, 13 samples were randomly sequenced.
Findings: No mutation was observed in the pertactin Region 2. In relation to the region1 of pertactin, 77% and 23% of the strains had prn2 and prn1 alleles, respectively. In relation to fim2 gene, 70% and 30% of the strains had fim2-2 and fim2-1 alleles, respectively. Also, in relation to fim3 gene, 70% and 30% of the strains carried fim3B and fim3A alleles, respectively.
Conclusion: In general, the present study results were similar to those of the previous studies conducted in Iran, but there were some differences in fim2 gene polymorphism so that the dominant allele changed from fim2-1 to fim2-2. Considering the fact that vaccine strains of Bp134 and Bp509 carry fim3A allele, which is different from the dominant circulating allele (fim3B), it is suggested that strains more similar to the dominant circulating strains should be used in designing vaccines.
Evaluation of Prokaryotic Diversity in Hypersaline Environment by Culture-independent Method

Volume 9, Issue 1 (Winter 2018)
Abstract
Aims: Microorganisms are present not only in common environment, but also in extreme environments. Salt lakes with near or at saturating salinity are spread all over the world. Urmia Salt Lake is one of these hypersaline environments. The present study aimed at evaluating prokaryotic diversity in hypersaline environment by culture-independent method.
Materials and Methods: In this experimental study, different regions of Urmia Lake were sampled and the genomic material extracted from the water sample was used as a pattern for the amplification of 16S rDNA and a fragment of the bop gene via polymerase chain reaction. By cloning, each of the amplified fragments belonging to a single strain was amplified by T/A cloning vector. To further investigate the biodiversity of Haloarchaea, the biodiversity of bop gene was studied in addition to studying 16S rDNA.
Findings: By cloning and sequencing, 6 bacteria genera, including Acaryochloris, Adhaeribacter, Brachybacterium, Gloeocapsopsis, Cesiribacter, and Bacillus were identified. Archaeal library belonged to 5 genera, including Halonotius, Halolamina, Haloquadratum, Halomicroarcula, and Halorhabdus. The clone libraries of bacterial belonged to 4 phyla, including Bacteroidetes, Cyanobacteria, Actinobacteria, and Firmicutes. . The clone libraries of bop gene (as a molecular marker) belonged to genera, including Halorubrum, Natrialba, Haloquadratum, and Natrinema. The bop phylogeny was closely related to the 16S rDNA phylogeny.
Conclusion: By cloning and sequencing, 6 bacteria genera, including Acaryochloris, Adhaeribacter, Brachybacterium, Gloeocapsopsis, Cesiribacter, and Bacillus were identified. The bop phylogeny is closely related to the 16S rDNA phylogeny.

Micromorphology of Pedogenic Carbonate Features in Soils of Kohgilouye, Southwestern Iran

Volume 14, Issue 1 (1-2012)
Abstract
The micromorphology of pedogenic carbonate features in calcareous soils of arid and semiarid regions of Kohgilouye Province, Southwestern Iran, has been studied to determine their genesis and distribution in different climatic regions. Eight representative pedons (from a total number of 24 pedons) were studied in aridic-ustic (minimum rainfall), ustic and xeric (maximum rainfall) soil moisture regimes. Micromorphological studies indicated that the frequency of secondary calcite as pedogenic nodules, coating or infilling in voids or channels increase from aridic-ustic to xeric soil moisture regimes. The presence of pedogenic calcite coating superimposed on clay coatings in pedons of more humid regions probably suggests a complex history of carbonate leaching, deposition of secondary calcite and clay illuviation. Pendants of calcite were observed in soils with coarser texture in aridic-ustic region as a common pedofeature. Pedogenic nodules in more developed soils of xeric regions were harder containing denser and more contiguous micritic calcite. Degree of impregnation of calcite nodules with Fe/Mn oxides as well as calcite depletion pedofeatures increase in areas with higher rainfall. Needle shaped calcite and cytomorphic calcite were observed in the near surface horizons of the regions with higher rainfall and denser vegetation growth of ustic and xeric soil moisture regimes.
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