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The aim of this research was to investigate the feasibility of composite and laminated chitosan (CH) and whey protein isolate (WPI) film preparation which was containing cumin essential oil and chitosan nanofiber. Production of composite film was possible by decreasing WPI pH to below its isoelectric pH. Double-layer was also prepared by pouring WPI solution on dried CH film. amount of active agent and nanofiller was fixed (125 mg/100ml) and only the effect of CH/WPI ration and film type (laminated or composite) on the properties of films was arudied by RSM analytical method. The color, watercontact angle, moisture absorption, solubility, water vapor permeability and mechanical properties of films were determined. By using desirability function and according to the results, optimum formulation of composite film (65.14% WPI and 34.85% CH) and laminated film (49.01% WPI and 50.99% CH) was achieved. Microstructure of optimized films was analyzed by FTIR, FE-SEM and XRD tests. Results of FE-SEM indicated a heterogenous stracture in composite film but without any phase separation. Also, at the laminatred film good attachment between two layers of CH and WPI was observed. Semi-crystalline stracture of composite film was approved by XRD analysis and there was no detectable difference between peak intensity and crystalline stracture of composite and laminated film. there was a slight difference in the FTIR spectra of composite and laminated films. According to the results, the characteristics of composite film in comparison to laminated film had better accordance with the used prediction model. But the laminated film exhibited better morphological characteristics and could be suggested for preservation of different foods due to its high potential for use in food packaging.

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Co-encapsulation of Lactobacillus acidophilus and Tuna oil rich in omega-3 fatty acids using Soy protein isolate (SPI) and Arabic gum (GA) as wall materials was studied through the complex coacervation method. The microcapsules were dried separately by freeze and spray drying. The optimal conditions for the coacervation between soybean protein isolate and gum Arabic as functions of pH, SPI/GA ratio and total concentration of biopolymers were investigated using zeta potential, turbidity, and coacervation yield assays. The highest coacervate yield was achieved in the total concentration of biopolymers 2% (w/v), SPI/GA ratio 60:40 and pH=4, and the highest coacervation yield was 79.22±1.75%. Lactobacillus acidophilus (La-5) viability was significantly (p˂0.05) higher in freeze-dried microcapsules in comparison with spray-dried microcapsules. Omega-3 fatty oil improved significantly (p˂0.05) the viability of probiotic bacteria during 60 days storage in ambient temperature and gastrointestinal conditions. The size of L. acidophilius containing microcapsules and co-microcapsules (SPI-P-O-GA) were 1.19±0.19 and 4.42±0.14 µm, respectively.