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Showing 2 results for Iranbakhsh


Volume 10, Issue 4 (11-2021)
Abstract

 Mosses have proved to have antifungal properties due to their specific chemical compounds. In this study, the different extracts of some mosses collected from Khuzestan province were tested on a phytopathogenic fungus Fusarium solani, an important pathogen of crops, especially wheat, and compared to the commercial fungicide Benomyl. For this purpose, the dried mosses samples of ethanol, methanol, and acetone were extracted. The antifungal activity was tested by the disc diffusion method, and the growth inhibition zone was measured. Wheat seeds of the “Chamran” cultivar were implanted into moss extract and then transferred into pots containing 1: 10 mixture of soil and soil contaminated with F. solani. After 35 days, the root and crown of wheat plants were examined based on the Wallwork scale. Ethanolic and methanolic extracts caused an inhibitory of 90% and 81% relative to Benomyl, while acetonic extract had fewer effects (76%) in the in vitro tests. In vivo observations had also indicated that ethanolic extracts can significantly control root and crown rot 63.8%.

Volume 24, Issue 4 (7-2022)
Abstract

Silver Nanoparticles (AgNPs) are widely used in medical and pharmaceutical applications due to their antimicrobial properties. In this study, Ag-NPs were synthesized using Viola tricolor aqueous extract as a reducing and capping agent. The presence of synthesized Ag-NPs was first confirmed with UV-Visible, SEM, TEM, XRD, and FTIR analyses, and then their antimicrobial characteristics were studied based on the Minimum Inhibitory Concentration (MIC). The SEM analysis showed that the synthesized AgNPs were spherical in shape. The particle size histogram revealed that the average particle size of the AgNPs was 49.45 nm. Findings from the FTIR and UV-Vis spectra showed the successful formation of Ag-NPs because the functional groups involved in the synthesis process and adsorption peaks were well developed. Furthermore, the Ag-NPs had a peak absorption at 420 nm in the spectrometry. MIC results showed the strong antimicrobial effects of the synthesized Ag-NPs. Results of the Minimum Bactericidal Concentration (MBC) revealed the dose-dependent cytotoxicity of the Ag-NPs. Nanoparticles could exert the inhibitory effect of DDPH free radicals in a dose-dependent manner. Methyl tetrazolium (MTT) results showed that silver nanoparticles had a dose-dependent cytotoxic effect and significantly reduced cell survival. The IC50 values for Ag-NPs and the extract were 11.83 and 204.4 μg mL-1, respectively. This study showed a higher cytotoxic effect of the green synthesized nanoparticles on hepatocellular carcinoma cells than the extract. Consequently, the biosynthesis of Ag-NPs using aqueous extract of V. tricolor is clean, inexpensive, and has antibacterial, anticancer and antioxidant activities. Hence, it can be used as a drug candidate.

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