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Background: Helicobacter pylori is the most common cause of chronic infection in the human stomach. The infection has universe prevalence in all age groups. Probably, this bacterium is the cause of most common chronic bacterial infection in human beings and infects approximately half of the world population. H. pylori produces urease, an enzyme that degrades the urea in the stomach’s mucous to ammonia resulting in biochemical reaction that leads to increase in pH of the stomach lumen. This allows pathogenic intestinal protozoa to take the opportunity to cross through stomach’s increased pH and cause disease. The aim of this study was to evaluate the relationship between H. pylori infection and prevalence of parasitic infection in patients in Ilam. Materials and Methods: Following stool samples collection during 2013 in patients with abdominal pain in Ilam, Iran. H. pylori infection was investigated based on stool antigen analysis (HPSA) by enzyme-linked immunosorbent assay (ELISA) method in patients who had recurrent abdominal pain. Stool specimens were examined using the direct examination and the spontaneous sedimentation method for detecting the trophozoite and cyst of parasites. Results: In this study, we found 65 patients with H. pylori infection. Out of these 65 patients, the percentage of patients with positive results for Giardia lamblia was 30.7% and for Entamoebahistolytica/dispar was 12.3%. Conclusion: The results of this study suggest that H. pylori infection may provide favorable conditions for giardiasis infection; however, this presumption needs further studies with larger sample size.

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Background: Streptococcus agalactiae, also known as Group B Streptococcus (GBS) is a commensal organism in the urogenital tract and rectum in approximately 25% of the healthy adult female population. The bacterium is the leading cause of bacterial meningitis, pneumonia, and sepsis in human infants.
Materials and Methods: Our study was performed over a three - month period from April to June 2014. Midstream specimens of urine were collected from outpatients suspected of having a bacterial urinary tract infection, which had not received any antibiotics. Group B Streptococci isolates were confirmed by typical colony morphology and identified by differential tests. Antibiotic susceptibility testing was carried out by disk diffusion method on Mueller Hinton agar (Merck, Germany) based on (CLSI) Guidelines 2012.
Results: GBS strains were isolated from 264 (21.1%) cases (out of 1249 positive bacterial urine cultures). The higher prevalence was recorded in the 15-44 and 45-64 age groups. Antibiotic susceptibility tests revealed that vancomycin, penicillin, and linezolid had the lowest, and tetracycline had the highest resistance rate.
Conclusion: In conclusion, the results of the present study confirm the universal susceptibility of GBS strains to the penicillin family and assert the use of penicillin or ampicillin as the first drug of choice for treatment and prophylaxis against GBS infections. However, it is important to perform antibiotic susceptibility testing whenever penicillin could not be prescribed.

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Companies have widely adopted the Internet and related technologies worldwide to conduct their business and improve performance. Internet technologies and electronic business provide new opportunities for companies to compete in the global market and play a significant role in the world economy. Despite the significant studies in e-business, there is a need for more comprehensive analysis in examining e-business in the sports industry based on new e-business approaches. This article aims to develop a theoretical framework of e-business based on the participation of sports consumers in the company's development, which was carried out with the qualitative research method and based on the foundational data theory and a constructivist approach. Research data were collected by a purposive sampling method using a snowball technique and based on in-depth semi-structured interviews with e-commerce. The number of research participants by using the theoretical saturation index reached 19. The analysis of research findings showed five main categories. Different dimensions of business, to make money for yourself and profit for the company. The final model of the research was presented so that the sports consumer can enter into business with companies through social networks by reaching electronic maturity and designing a project or service if he has high communication literacy. It was found that sports consumers can participate in developing sports or non-sports companies that either need online advertising are advertising agencies. These sports organizations need to hire people or sports manufacturers that need feedback on their products.

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Students are the country's future makers and the university's existential philosophy. Participation in sports activities can positively affect students' liveness and vitality. The purpose of this study was to present a constructivist theory in line with student participation in sports and physical activities, which was done with a qualitative research method and a heuristic-fundamental nature. Through the purposeful sampling method and snowball sampling technique and based on interviews with 17 experts with experience in university sports, research data were collected, and data were coded and analyzed based on the theoretical foundation of data and with Charms' constructivist approach. The findings showed that four operational factors: budget allocation, management, human resources, technology, and infrastructure, and two mediators of development environment and development resources have a significant role in student growth and desire to participate in sports. As a result, a baseline theory based on medium-range data, which is the essence of the theoretical foundation of data, called " general to specific growth pattern of student in sport, "was explained aiming at changing behavior and institutionalizing a desirable habit based on the active participation in sports for students. It was found that the components of university sports become in the form of the university sports components roles, taking into account the development of stimuli through mediators and operational factors, and the development of university sports in Iran is not far away if this cycle is implemented properly.
 

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In order to improve the properties of myofibrillar protein-based film from silver carp (Hypophthalmichthys molitrix), nanofibrillated cellulose (NFC) at 3 levels (1, 3 and 5%) was used. Optimum treatment was determined by evaluating the mechanical, physical and optical properties as well as scanning electron microscopy analysis (SEM). Cellulose nanoparticles had no effect on tensile strength but reduced the elasticity of film (p≤0.05). Water vapor barrier property (WVP) and other physical properties of the films were improved by addition of nanofibrillated cellulose at 1%, but decreased at higher concentrations (p≤0.05. Based on SEM, low concentrations of nanoparticles showed more homogeneous dispersion and films had a smoother and better cross-sectional area compared to the higher levels of nanoparticles. Generally, low levels of nanoparticles could be effective to improve the mechanical and physical properties of myofibrillar protein - nanofibrillated cellulose films.  

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The purpose of this study is to analyze the effects of different fat levels and substitution of sodium chloride salt with potassium chloride on physic- chemical and sensorial properties and shelf life of fish sausage produced from minced fish in the refrigerator. To do this, the sausages have been produced with different fat levels (10, 15, 20 %) and substitution of part of sodium chloride with potassium chloride( 0.95 KCl+ 0.54 NaCl and 0.47 KCl+ 1.02 NaCl)and have been reserved in refrigerator for 30 days and physical, chemical and sensorial evaluations have been done. The results of analyzing the approximate composition and PH show salient differences between different formulations of sausage and the control sample (p<0.05). By adding KCl to formulation, the final products had higher harness in comparison to the control sample. Sensorial analyze didn't show any significant difference between the samples. Treatment with higher fat content got higher score in the textural properties but this difference was not significant (p> 0.05). There was not a salient difference in color, taste and total acceptance between samples and control sample. Generally sensorial properties reduced in time but this reduction was not significant. Increase of thiobarbituric acid has been observed in all treatment over time but this amount never violated the normal range in any treatments.

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The egg parasitoids of the genus Trichogramma have become one of the most important economic insects, for they are widely used in biological control of Lepidopteran key pests. For that reason, all aspects of their mass production including diapause have been considered over the last few years. In this study, the effect of host quality on the diapause induction of sexual and asexual T. brassicae Bezdenko (Hym: Trichogrammatidae) have been investigated at two temperatures. Maternal generation developed at two distinct temperatures (14 and 20 °C) and they oviposited on four different qualities of Ephestia kuehniella Zeller (Lep: Pyralidae) eggs including fresh eggs, frozen eggs, host eggs with developing embryo and low quality eggs. Data analysis revealed significant influence of temperature and host quality on diapause induction in both sexual and asexual strains. Maximum diapause happened in host eggs with developing embryo even though they were in fetal development. A higher level of diapause was observed in T. brassicae whose maternal generation had developed at 20 °C. The diapause induction in asexual T. brassicae was less than sexual wasps. The results imply that Wolbachia causes a disturbance in the diapause process. The outcomes of this study, which are new, indicate the complexity of diapause and the importance of factors such as host quality which have received less attention in diapause induction. 

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After the success of the revolution in the Islamic Republic of Iran, all polices regarding crude oil production and marketing have been revised and changed. Since the policies and methods of crude oil marketing were always influenced by political and economic conditions, and since the Islamic Republic of Iran has experienced different political and economic situations after the revolution, we tried in this paper to discuss the policies and ways of marketing and selling the crude oil in Iran. For finding the effective factors on the Iranian export crude oil prices from year 1979 (Islamic Republic of Iran revolution) till year 2005 and prioritizing them, we used Likert scale, but since the crude oil marketing experts couldn’t show the priority of effective factors by Likert Questioner, we used Analytical Hierarchy Process (AHP) to prioritize the factors. The findings show that, the quality of crude oil (API and Sulfur content) is the most important factor and “the General Terms and Conditions (GTC)” of National Iranian oil company ( NIOC) for selling Iranian export crude oil is the less effective factor on the Iranian export crude oil prices.

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The aim of present study is to assess modularity of language. To this end, we measured the ability of children with specific language impairment in pragmatic and grammar and compaired them with normal children.  First we used narrative speech, language development and specific language impairment tests for diagnosis, then 6 children with specific language impairment were compared with 6 age-matched and 6 language-matched normal children in their grammar and pragmatics. Independent t-test, Man Whitney and Wilcox tests were used for analyzing data. It is shown that the children with SLI perform much lower their language-level with respect to grammatical properties (time agreement), but in pragmatic properties (reference) they don’t have meaningful differences. The result of this study shows that impairment in grammar beside good performance in pragmatic provides support for modularity of language.                        

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Autism is the most concrete developmental disorder. Upon recent epidemiological studies, the prevalence of autism is about one in every 165 children. Only 25% of them have intellectual disabilities. So, it was thought that the disorder is not necessarily with severe cognitive damages. While the poor performance of children's communication is the main features of them, language profile of symptoms varies from person to person. On the one hand, children have ability to perform their language as the norm, and on the other hand, children who do not have the ability to speak. So, Language impairment is one of the main characteristics of children with autism; however, findings of the language deficits in these children have been inconclusive, and even less is known about the language profiles of Persian children with autism. Due to the heterogeneity in autism, we also need extensive investigations on the properties of their language in different languages. The aim of this study was to evaluate grammatical characteristics in children with autism by revealing the differences between age-matched and language-matched normal children.
Rizzi believes each structure will consist of three layers from top to bottom as follows: The complementizer phrase (CP), inflectional phrase (IP) and verbal phrase (VP). So, the Complement layer is syntactic materials to build question phrases, complement phrases and relative clauses. Inflectional layer is related to inflection, negative and auxiliary verbs while verb phrases are related to verb and its arguments.
Research data were collected at 10 autistic (ages 6 to 9) and 20 normal children (10 age-matched and 10 language-matched). First, we used The GARS-2, and the ASSQ for diagnosing high-functional Autistic children. The GARS-2 is a norm-referenced instrument that assists teachers and clinicians in identifying and diagnosing autism individuals aged 3 years to 22 years and in estimating the severity of the child's disorder. The ASSQ is a questionnaire which was filled out by the parents or teachers of children or adolescents (6 to 17 years of age). It stands for screening autism spectrum disorders. Children’s expressive grammar was measured by using the Persian Photographic Grammar Expression Test. It is the first reliable and valid test that exclusively and accurately evaluates grammatical characteristics of Persian-speaking children. It concludes 32 grammatical structures it has good content validity (CVI>80), convergent validity of two test (r P= 0.5) and a correlation coefficient equal to 0.91 and greater than 0.9 respectively. Also, the test has a good internal consistency (kr21= 0.82). And finally data were analyzed with Mann-Whitney test.
 The result of this research showed that there is significant difference between autistic group and age-matched group in all structure except in WH question, yes/no question, different type of relative clauses, tense, aspect, mood, different type of pronouns and preposition. But comparison between autistic group and language-matched group showed that there is no significant difference in most structures except WH question, yes/no question, complementary relative clauses, different type of pronouns and preposition. Overall, autistic children demonstrated consistent expressive impairment in questions, relative clause and pronouns. It should be noted that these factors should be seriously considered in identification and treatment of this phenomenon.
 

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Colorectal cancer (CRC) is one of the most common cancers worldwide. Understanding of the tumor behavior, in a much closer look, at the molecular level, results in a more effective treatment and accurate prognosis of the disease. From among various genes altered in colorectal cancer K-ras is assumed to be of diagnostic and prognostic significance. K-ras mutations are believed to be a critical event in colorectal oncogenesis. Previous studies have demonstrated that 40% (20-50%) of CRCs harbor a mutant allele of K-ras oncogene. The mutations are limited to codons 12, 13, and 61 of the gene, with a great incidence at codon 12. The localization of mutations has given mutated K-ras an advantage of sensitive and simple detection over APC or p53 in which mutations are spread in their whole DNA sequence. To determine the incidence of K-ras mutations in CRC in Iran, compared with other countries, DNA was isolated from a random collection of 55 colorectal carcinoma samples, and codon 12 K-ras mutations were detected by RFLP. K-ras mutations in sporadic colorectal cancer in Iran are relatively frequent, with an incidence of 65%. This may be attributed to variation in methodology and to characteristics of the population studied such as differences in genetic background and variability in environmental factors and epidemiologic parameters such as diet, social lifestyle status, and other parameters that could be specific to the Iranian population. Correlation between the presence of codon 12 mutation and various clincopathological parameters was also investigated. A significant correlation was found with poor tumor differentiation of tumor samples. This places much emphasis on the role of promotion of differentiation as the most prominent effect of Ras.

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The performance and flight behavior of the potato aphid Macrosiphum euphorbiae was studied on the 'Superior-BT' line transgenic for the CryIIIA toxin of Bacillus thuringien-sis (BT), resistant to the Colorado potato beetle; and non transformed 'Superior' line which served as control. Mortality of the treated aphids was negligible and potato lines did not affect the development time of M. euphorbiae, but aphids were largest on 'Supe-rior' and smallest on BT potatoes. This difference was reflected in aphid fecundity, which was lowest on 'Superior-BT', and highest on Superior. Incidence of flight in newly emerged alate M. euphorbiae that developed on BT was high compared to control. The re-sults illustrate that the performance of a secondary pest of potato can be unpredictably affected by the resistance factor involved in developing specific resistance to a primary pest.

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Mediterranean flour moth, Ephestia kuehniella Zeller, is important for mass rearing of parasitoid insects such as Bracon wasps and it's feeding on cereals leads to economic losses in flour mills. In current research, the effects of four different diets: wheat, barley, oat, and maize flours, on protein content of larval and pupal whole-body, gut and fat bodies of the last instars and the digestive α-amylase activity were evaluated. In addition, their protein patterns were compared using polyacrylamide gel electrophoresis (SDS-PAGE). The protein contents of fifth instars whole-body, fat bodies, gut, and also pupa varied in different diets. On the whole, the lowest protein levels were found in all experiments in oat and the highest in barley and wheat. Other biological parameters such as insect weight and digestive α-amylase activity were also significantly affected by the decrease in protein content. The results obtained from the estimation of protein content by a colorimetric method and SDS-PAGE were consistent with each other; the low or high protein contents were also clearly visible in the gels. According to the results of enzyme activity and protein contents of insects reared on different diets, barley and wheat are reported to be appropriate diets for this pest, while oat and maize are not suitable diets.
 

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Objective: DNA markers are one of the most important indicators for estimating Molecular weight of DNA samples, although it used in widespread medical and research laboratories. These markers are very divers and have been prepared in different manners and from different sources of DNA. But unfortunately, DNA markers haven't been made in our country and all of the markers that we use are made in a foreign country. The aim of this research is settings a suitable technology to produce this product in the lab. Material and Methods: With this aim, we used two different strains of lambda: c1857sam7 and EMBL3A both of which are lytic phages as a DNA source. These were grown in the suitable host, after plaque appearance on the bacterial lawn, suitable titer for phage collecting was determined. We also optimized plasmid purification method for extraction of pBR322, pUC18 and recombinant VZV plasmid DNA and designed fragments in the markers have been constructed by digesting these DNAs with variant enzymes. Results: In this study, we made seven DNA markers out of which four of them were made for the first time in the world (/Hind III/BamH1, /Hind III/EcoR1, Sam2, Sam1) and although foreign models of three of them exist but they were made in our country for the first time (/Pst I, /Hind III, pBR332/MspI). Conclusion: The other goal of this study was to determining the best conditions for maintaining and preserving these markers in the lab which was successfully performed.

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Objective: Hepatitis C virus is the major cause of viral hepatitis and its diagnosis in suspected specimens is of great importance. The risk of transfusion- transmitted virus infection is primarily the result of failure in serological screening tests to detect recently infected donors in the pre-seroconversion window period of infection. Therefore, sensitive and accurate diagnosis of HCV prior to antibody production to reduce window period is necessary. Materials and Methods: In the present study, a sensitive and specific RT-Nested PCR method for detection of a conserved HCV 5'UTR sequence was developed. Two pairs of primers for amplification of the target sequence in two rounds of PCR were selected. The developed RT-Nested PCR assay was performed on HCV-antibody confirmed positive samples as well as negative controls and standard samples. In order to compare the results, One Step RT-PCR kit was used in this study. Results: 25 HCV-positive plasma samples whose positivity were confirmed by ELISA and Western Blot tests, also as well as 10 fold dilutions of a high viral load plasma sample obtained from a HCV-positive patient as standard samples and 25 negative control plasmas from healthy blood donors were collected and tested by this assay. In all of positive samples a 175bp band was observed on agarose gel electrophoresis, but no band could be detected in negative control plasma. Results from developed RT-PCR assay and One Step RT-PCR kit showed a good correlation. Conclusion: According to the results of this study, the developed RT-Nested PCR assay has a good sensitivity and specificity for diagnosis of HCV infection. It has the advantage of viral genome detection prior to seroconversion and can be used to detect HCV infection during window period

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Since there is no special reading and writing book for the Persian children with autism and also their cognitive and linguistic abilities are different, the investigation of the effective factors on their reading skill is an important step for evaluating and modifying their using pedagogical books. In the other hand, Persian language has a different orthography and grapheme–phoneme correspondence from other languages (English, Italian...), so the effect of phonological awareness in it΄s reading is also different. Therefore, it’s necessary to identify the correlation between phonological awareness and reading in Persian children with autism. In this research, 26 children with autism and 30 normal Persian children living in Tehran were participated. Two tests (phonological awareness and reading) were performed and the results were analyzed by statistical tests. Results showed that in the group with autism, there is a meaningful correlation between word reading and phoneme combination and same first Phoneme recognition, and between non word reading and alliteration, phoneme segmentation, first and final phoneme deletion. In control group, there is a significant correlation between word reading (and non-word reading) and the onset and rhyme awareness, phonemic awareness, alliteration, same first phonemes, first phoneme and middle phoneme deletion. Also, there is a significant difference between the children with autism and control group in all subtests except for the syllable segmentation (0.075) and phoneme combination (0.163). Therefore, the degree of skill and correlation in the children with autism is different from the normal children and this should be noticed seriously in teaching reading.
 

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The Sunn pest, Eurygaster integriceps (Puton) (Hem.: Scutelleridae), is an economic pest of wheat that reduces the quantitative and qualitative properties of wheat products. We investigated the effect of E. integriceps feeding on six different wheat cultivars (Ghermez (Red), Noodle, Antanius, Sabalan, Azar 2 (with two types of cultivation), and Sardari) on the protein content in the adult’s gut and fat body and their digestive enzymatic activity. All qualified values of the insect feeding on wheat cultivars differed significantly. The least amount of adult weight and protein content of gut and fat body were observed in the insects fed on Ghermez (Red) cultivar, and the highest amount belonged to the Sardari cultivar. The same results for protein content were obtained from SDS-polyacrylamide gel electrophoresis (SDS-PAGE). Also, the lowest and highest gut α-amylase, pectinase, and protease activities were in Ghermez and Sardari cultivars, respectively. Therefore, it was concluded that the type of wheat cultivar affects the food preference of this insect and, thereby, physiological parameters of the insect gastrointestinal tract. Planting a wheat cultivar like “Ghermez,” which may be resistant, can be a suitable and cost-effective method to decrease the chemicals applied against this pest.

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Klebsiella pneumoniae is a gram-negative bacillus of the Enterobacteriaceae family. Despite being part of the natural human microflora, this is an opportunistic pathogen and a major cause of nosocomial infections. The increased emergence of multidrug resistance in Klebsiella pneumoniae has limited the treatment options for this bacterium. Carbon nanotubes (CNT), by improving the stability and solubulity of drugs, could increase the effectiveness of drugs for treatment. The aim of this study is to investigate the antibacterial effect of nanofluid containing functionalized multi-walled carbon nanotubes (f-CNT-NF) on Klebsiella pneumoniae isolated from clinical specimens. For the strain confirmation, biochemical ,API20E kit, and additional differential tests were performed, and antibiotic susceptibility test was performed by the disk diffusion method. The studied strain showed a resistance to all antibiotics such as cefepime.The minimum inhibitory concentration (MIC) was determined using the antibiotic micro dilution method. The MIC was determined in five effect modes including antibiotic (Ab), nanofluid containing functionalized multi-walled carbon nanotubes (f-CNT-NF) , nanofluid containing multi-walled carbon nanotubes (CNT-NF) ,Ab in combination with f-CNT-NF and Ab with CNT-NF. Nevertheless the individual effects of 10 µg mL^-1 cefepime or 80 µg of nanofluid with f-CNT-NF did not inhibit the growth of the bacteria, but the co-administration of 10 µg mL^-1 cefepime with 80 µg of the f-CNT-NF could inhibit the bacteria`s growth. It was concluded that f-CNT-NF could be more effective in drug delivery at lower concentrations than the free state, which could be used as a tool for optimal drug delivery.