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Showing 2 results for Haghighifar


Volume 6, Issue 2 (Spring 2020)
Abstract

Aims: Acinetobacter baumannii is an opportunistic pathogen that is resistant to many antibiotics including beta-lactams. Production of β-lactamases is the main mechanism of β-lactam resistance in A. baumannii strains. The aim of this study was to determine the frequency of blaTEM and blaVEB genes in clinical isolates of A. baumannii and the relationship between the antibiotic resistance and the presence of ESBL genes in strains isolated from burn wound infection in Isfahan.
Materials & Methods: In this study, 123 MDR A. baumannii strains were isolated from burn wound infection. After antibiotic resistance evaluation using the Kirby-Bauer disc-diffusion method, all the isolates were evaluated with polymerase chain reaction (PCR) technique to detect ESBL genes, followed by statistical analysis by the end.
Findings: Out of 123 A. baumannii isolates, 77 (62.60%) strains were ESBL positive according to the PCR results. The frequency of blaTEM and blaVEB genes was 52 (42.3%) and 67 (54.5%), respectively. There was a significant relationship between the antibiotic resistance and the presence of ESBL genes (blaTEM and blaVEB) in A. baumannii strains.
Conclusion: The high prevalence of blaTEM and blaVEB genes in A. baumannii strains found in this study is the major concern about burn wound infections in Isfahan and Iran because of the complexity in treating infections caused by these strains. This study results highlighted the need for infection control measures to prevent the spread of resistant isolates and ESBL genes, especially in burn hospitals.

Volume 8, Issue 3 (Summer 2022)
Abstract

Backgrounds: Uropathogenic Escherichia coli is a Gram-negative bacillus that is the most common cause of urinary tract infection. E. coli has the ability to produce biofilm as an important virulence factor. Due to the lack of sufficient information about ESBL resistance genes in this geographical area, this study aimed to investigate the prevalence of ESBLs in E. coli isolates to increase our knowledge about the role of these genes and biofilm formation in inducing resistance.
Materials & Methods: 139 E. coli strains were isolated from urine samples. Antibiotic susceptibility testing was performed for the isolates by disk diffusion method. ESBL production was confirmed using double-disk synergy test. Molecular detection of ESBL genes was performed using PCR. Biofilm formation assay was performed by microtiter plate method.
Findings: The most effective antibiotic against this bacterium was nitrofurantoin. Multidrug resistance was observed in 119 (85.6%) isolates. ESBL phenotype was detected in 93 (66.9%) isolates. The PCR test results showed that blaCTX, blaVEB, and blaTEM were positive in 45 (32.4%), 87 (62.6%), and 10 (7.2%) isolates, respectively. The biofilm formation assay results revealed that 65 (46.8%), 58 (41.7%), 10 (7.2%), and six (4.3%) isolates were non-, weak, moderate, and strong biofilm producers, respectively.
Conclusions: The high prevalence of ESBL genes is a public health concern in this region because they could be transmitted to other susceptible bacteria and induce resistance. This study showed that biofilm production could increase antibiotic resistance.
 

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