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The effect of Spirulina powder at levels of 0 (control), 3, 7, and 10%, of food ratio on growth, survival, and total carotenoids of dwarf gourami fry (Trichogasterlalius) of 3.6 g average weight as well as its larval stage was investigated for a period of 8 weeks. Carotenoids was significantly increased with increase in spirulina level up to 7% (p<0.05), but not significantly different from 10% level (p> 0.05). Survival showed no significant difference (p> 0.05). In larvae, survival, total carotenoids and total length increased significantly with increasing spirulina powder (p<0.05). Overall, the spirulina powder at 3% improved the growth and total carotenoids factors in pre-adult gourami and 10% level for larval stage as the best treatments in this study.

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Bacteria are used directly by zooplankton as a food source. The purpose of this study is to use straw as a substrate for the proliferation of bacteria in geomembrane pools and its effects on the production and biochemical compositions of Artemia. One week before Artemia culture, the pools inoculated with Dunaliella sp. and straw (particles less than 200 microns) as follows: treatment 1= geomembrane pool containing Dunaliella sp. (one week of algae cultivation), treatment 2 = geomembrane pool containing Dunaliella sp. and 0.5 grams per liter of straw (one-week algae cultivation and adding straw from the second week), treatment 3 = geomembrane pool containing Dunaliella sp. and 0.5 g/l of straw (algae cultivation and adding straw from the first week) and treatment 4= geomembrane pool containing 0.5 g/l of straw (adding straw from the first week). Artemia was cultured for 18 days and fed based on a turbidity depth of 15 cm. The highest growth rate was observed at the end of day 18 in treatment 3 (P<0.05). The highest amount of protein was obtained in treatment 1 (P<0.05). The highest amount of biomass was observed in the control and T3 groups (P<0.05). The presence of straw in Artemia pools decreased EPA fatty acid by 5-7 times less than the control (P<0.05). Artemia can grow while feeding on environmental bacteria. Applying straw in Artemia cultivation can lead to an increase in PUFA fatty acids with 18 carbon chains.