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The effectiveness of technology transfer is defined as fulfillment level of organization goals. Organizational culture, which affects from national culture, can underlie the success or failure of the technology transfer process. For this purpose, this study examined national and organizational role in technology transfer effectiveness. Accordingly, in order to validate the model, managers and experts in technology-oriented companies that have related experience in international technology transfer, determined as statistical population. 89 managers and experts in various fields of technology transfer were analyzed as the study samples. Findings of the hypothesis reflect national and organizational culture role in technology transfer (to determine the success and failure). Results also showed that organizational culture has mediating role in relationship between national culture and technology transfer effectiveness.