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Recently, the design and production of biodegradable films have received special attention than synthetic packaging due to the reduction of environmental pollution. The aim of this study was to investigate the effect of Persian gums (1%) and gellan gum (1%) on the physical, mechanical and morphological properties of composite films based on sodium caseinate (10%) as film reinforcing agents. The films were synthesized by solvent evaporation and the effect of each gum on the characteristics of the composite films was evaluated. The results showed that the addition of gums strengthened the composite films. So that the composite films showed mechanical resistance and good barrier properties versus moisture and light. The surface properties and morphology of the films also showed that the gums were well computability to the casein film and formed uniform and stiff films. In addition, composite films had acceptable transparency. Thus, it can be concluded that the use of composite films and the combination of different polysaccharides with protein matrices can improve the properties of the resulting films. On the other hand, by adding antimicrobial and antioxidant agents to biodegradable films, they can be considered as active packaging.

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In this study, different concentrations of aqueous extract of Chubak root (at levels of zero, 0.5, 1, 1.5, and 2%) with different concentrations of rosemary essential oil nanoemulsion (zero, 0.5, 1, 1.5, and 2%) was used to optimize the production of biodegradable gelatin-based film. Test responses were statistically significant for all fitted regression models at 99% confidence level. To optimize film production, maximum transparency, contact angle, tensile strength, Elongation at Brake to the point of rupture and minimum solubility, swelling and vapor permeability were evaluating. After fitting the models, the results showed that the effect of aqueous extract of Chubak root and rosemary essential oil nanoemulsion on all responses was significant (P <0.05). Increasing the aqueous extract of Chubak root and nanoemulsion of rosemary essential oil increased the thickness, contact angle and Elongation at Brake to the point of rupture and decreased moisture content, vapor permeability, transparency, solubility, tensile strength and swelling. Based on the results of model prediction and comparison with experimental values, aqueous extract of Chubak root at a concentration of 2% and nanoemulsion of rosemary essential oil with a concentration of 0.77% were obtained as optimal values.

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In this study, biodegradable packaging based on gelatin biopolymer (at a concentration of 3% w/w) containing nanoparticles of titanium dioxide (at a concentration of 1% w/w) and saffron extract (at a concentration of 2% v) by evaporation method was synthesized. In this study, physical properties (thickness, transparency, moisture content, solubility and water vapor permeability), mechanical, antimicrobial and antioxidant properties, structural and transparency properties of films were investigated. After analyzing the data, the results showed that the effect of saffron extract and titanium dioxide nanoparticles on all the studied properties was significant (P <0.05). Addition of titanium dioxide nanoparticles and saffron extract increased the thickness, improved the mechanical properties and reduced the moisture content, water vapor permeability, transparency, and solubility. Also, nanocomposite films containing titanium dioxide nanoparticles and saffron extract showed antioxidant properties (% 80%) and acceptable antimicrobial effects, especially against gram-positive Staphylococcus aureus bacteria. According to the results of this study, this type of packaging can be suggested as a suitable alternative to synthetic packaging.