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In this study, the effectiveness of 4 killed VNN vaccines along with three types of adjuvants was evaluated by both immersion and injection methods. About 540 fish weighing 7-10 g of ozone (Acipenser stellatus Pallas 1771) were considered. Vaccination was performed in two stages one month apart, and one month after the second recurrence, exposure to the acute live virus was performed. During this period, the mortality rate of immersion and injection groups was 12.9% and 19.8%, respectively, compared to 100% mortality in the control group. Blood sampling was performed to assess immune factors (superoxide dismutase, lysozyme) in four stages before the first vaccination in the adaptation period, after the first vaccination, after the second vaccination one month and after exposure to live virus Acute was performed to identify changes before and after exposure to the virus. The results of the present study showed that immunization vaccination in the vaccinated group with the vaccine containing IMS 1312 SEPPIC adjuvant significantly higher levels of superoxide dismutase 1745 IU / MI (p<0.05) and lysozyme 40.6 (p<0.05). Compared to other groups, which proves its better efficacy compared to other vaccines. Therefore, a vaccine killed with 75% IMS 1312 SEPPIC adjuvant can be recommended for vaccination against VNN.

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Today, the use of self-healing concretes has attracted the attention of various researchers. In this paper, the effect of Bacillus subtilis bacteria with different concentrations were used for evaluation on concrete containing zeolite.The mentioned bacteria were produced in the laboratory and added to the concrete mixing plan. In order to evaluate the effect of Bacillus subtilis bacteria on specimen with zeolite, resistance and durability tests including compressive strength, ultrasonic, water penetration test and water absorption tests were performed on the specimen at different ages. Also, using scanning electron microscope (SEM) and optical microscope, the self-healing of concrete was evaluated. The results of the compressive strength test showed that the specimen containing zeolite with a concentration of 2.8×10⁸ cells/ml increased the compressive strength by 16.76% and the specimen without zeolite increased the compressive strength by 13.51%. Also, the presence of Bacillus subtilis bacteria in the mixing design led to a 15% decrease in water absorption of specimens without zeolite and 30% of specimens with zeolite. Based on the results of the experiments, the most suitable concentration, for the simultaneous improvement of the resistance parameters and durability among the different concentrations of Bacillus subtilis bacteria, is suggested to be 2.8 x 10⁸.

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Various culture media contain a variety of materials that affect plant growth and development. Finding the best media culture among the various materials is thus critical to plant productivity. This study was conducted based on a completely randomized design using eight treatments and three replications. The treatments included the ratios 30:10:60 of perlite-vermicompost-coco peat, peat moss-vermicompost-palm peat, coco peat-vermicompost-palm peat, perlite-vermicompost-palm peat, and the ratios 30:70 of peat moss-palm peat, coco peat-palm peat, perlite-palm peat, and vermicompost-palm peat. Cucumber seeds were planted in pots containing these culture media under greenhouse conditions. After the four-leaf stage, the morpho-physiological responses of the seedlings were evaluated. According to the findings, when compared to perlite-vermicompost-coco peat medium, peat moss-vermicompost-palm peat increased shoot fresh weight by 5.5-fold, root fresh weight by 4.5-fold, root dry weight by 5.8-fold, shoot dry weight by 7-fold, stem diameter by 1.7-fold, shoot length by 2.3-fold, root length by 1.3-fold, and leaf area by 3.8-fold. However, compared to seedlings grown in peat moss-vermicompost-palm peat medium, the amount of auxin and reactive oxygen species decreased while total soluble sugars and peroxidase increased in perlite-vermicompost-coco peat medium. Our findings indicate that the composition of peat moss-vermicompost-palm peat can be used as a beneficial medium to improve quality of cucumber seedling under greenhouse conditions.