Showing 88 results for علیزاده بهبهانی
Volume 13, Issue 51 (7-2016)
Abstract
The aim of the study was to evaluate antimicrobial effect of extract of Lamiaceae plants (Thymus vulgaris L., Mentha spp. and Ziziphora tenuir L.) to prevent growth of pathogenic bacteria (Staphylococcus aureus, Geotrichum candidium) was studied industrial Doogh samples. For this purpose, three levels of concentration of extract that containing (0, 0.075, 0.15 v/v) was prepared. Survival or decrease in the bacterial population in 40 treatments (3 times repeated) in the samples of sterilized Doogh, which that contain a suspension of pure specific strains of Staphylococcus and Geotrichum in the during 24 hours and 14 days by measuring the kinetics of bacterial pathogens was investigated using response surface design. Analysis Results in the inhibition effects of natural antibiotic agent in the Doogh samples revealed that concentrations of thymus extract % 0.14 (v/v) , Mentha oil % 0.11 (v/v) and Ziziphora oil % 0(v/v) is the inoculation rate, which in most circumstances Reduction Staphylococcus aureus in 24 h 2.84Log/ml, 7 days in the 1.63 Log/ml
Volume 13, Issue 52 (4-2016)
Abstract
Mespilus germanica as a valuable medicinal plants used in traditional medicine. Aim of this study, investigated effect of combination various ratio of solvents (Glycerin, Ethanol, Methanol and Water) on the efficiency of Mespilus extract by mixture optimal design. Numerical optimization was used to obtain the optimal formulation of solvent. At the end of the day, the antimicrobial effect of Mespilus extracts based on three methods (agar diffusion Method, Minimum Inhibitory Concentration and Minimum Bactericidal Concentration) on the three microorganisms managing infectious diseases was investigated in vitro. In this study, investigated effect water, ethanol, methanol and glycerin on the five levels (0, 31.25, 83.33, 125,250 mm) on efficiency of Mespilusextracts by mixture optimal design. Diffusion agar test, Minimum Inhibitory Concentration, Minimum Bactericidal Concentration by microbroth dilution method was used to determination Susceptibility of bacterial isolate. The Result indicated that Scheffe polynomial model was highly significant for efficiency of Mespilusextracts. Minimum Inhibitory Concentration of Staphylococcusaureus, Escherichia coli, Pseudomonas aeruginosa were 1.25, 2.5 and 5 mg/ml, respectively. The optimum condition has been found as following: glycerin (0 ml), water (23.7 ml), methanol (100.2ml) and ethanol (126.1 ml) respectively. It’s worth to mention that there was no significant difference between experimental and predicted value in optimum condition. Mespilus extract was highly significant for reduce of Infectious bacteria. Mixture methodology based on the D-optimal design was able to statistical assessment extraction process with the minimum experiment.
Volume 13, Issue 53 (5-2015)
Abstract
The aim of this work was identified lactic floraas a traditional fermented food and then evaluates antimicrobial activities of some strains. A total of 140 Gram-positive and catalase-negative isolates were subjected to grouping by physiological and biochemical tests and carbohydrates fermentation. Based on the resultsthese 140 isolateswere dividedinto 9 groups. Two or three isolated were selected from each group and 16S rRNA was amplified using universal primers. Diversity of lactic acid bacteria in horreh was as followings:Lactobacillus fermentum (30.00%),Lactobacillus plantarum (28.57%), Lactobacillus brevis (15.00%), Weissellacibaria(8.57%),Enterococcus (faecium and faecalis) (7.14 %), Leuconostoc (citreumand mesenteroides subsp.Mesenteroides) (6.42%) and Pediococcus pentosaceus (4.28%). Antagonistic activity of 20 isolates (strains) of lactic acid bacteriaobtained fromhorreh was evaluated against food- borne bacteria. Sixteen isolates in Agar spot method and 14 isolates in well diffusion assayshowed antibacterial activity against at least one of these indicators. Eight isolates including:Ent. faecium (1), Ent. faecalis (1), P. pentosaceus(1) and Lb. plantarum (2) exhibited the highest antagonistic activity toward Listeria innocoa. Antagonistic activity of cell free supernatant (CFS) from Lb. plantarum showed the highest thermal stability. Also, two isolates belonging to:Ent. faecium, Ent. Faecalis presented antibacterial activity at pH=7. Only, the supernatant of Lb. plantarum was not influenced by proteinase K.The results showed that the supernatant of some isolatestestedcan be used as a bio preservative in food products.
Volume 13, Issue 53 (5-2015)
Abstract
The purpose of this study was to evaluate the effect of time, kind of extract, extract concentration and temperature on the dynamically the population of Escherichia coli (infectious agents) in the complex food system (frankfurter sausage) and Genetic Algorithm - Artificial neural network and neuro fuzzy system (CANFIS) were used for dynamic modeling of population of E.coli. At this research laboratory, Minimum Inhibitory Concentration (MIC (and Minimum Bactericidal Concentration (MBC) of aqueous and ethanolic extracts were studied using the micro broth dilution method. GA- ANN, neuro fuzzy (CANFIS) were fed with four inputs: concentration at the five level (0, 2000, 4000, 6000, 8000 ppm), kind of extract ( watery, ethanol), temperature at the three level (5,15, 25 ̊С) and time (1-20). The results showed that the ANN with 1 hidden layer comprising 10 neurons, Tangent hyperbolic function, momentum training rule and percent of used data 30/30/40 for training/cross validation/testing respectively gives the best fitting with the experimental data, which made it possible to predict with high determination coefficient (R2 equal to 0.995). Also the correlation between CANFIS predictions and experimental data was very good (R2 equal to 0.96). It's worth to mention that in this research GA- ANN was better approach to simulation dynamically the population of E.coli.
Volume 13, Issue 54 (8-2015)
Abstract
Kimchi is a fermented herbal supplement, and appetizer that according to a raw material, process and geographical location are classified more than161 types. In this study, after kimchi production, isolation and identification of microorganisms was performed by molecular method. Lipase producing strain, Trichosporon asahii was isolated from kimchi sample (19.01 ±3 U/ml). The various medium components and culture parameters to achieve a more cost effective and economically viable bioprocess were screened and optimized using Design Expert software. PBdesignsare used to screen the most effective variables on lipase production that fermentation temperature and initial pHfor 48 hours 30 . According to the results, extract of nigella sativa, olive oil, yeast extract, magnesium chloride, respectively, were the most variable. Variable particle size and pepton ehavenegative effect. The variable selection and optimization on was performed more efficiently. Finally, lipase activity was 35 ± 0.5 U/ml in the optimal conditions using a medium containing15% sativa extract, 10 g/l yeast extract, 22.5 g/l olive oiland25 mM/l magnesium chloride in the rotation speed of 150 rpm, respectively, as well as enzyme activityafter84/1times the optimal state before optimization. The kinetic parameters V (max) and K (m) was 0.367 mM / min and 0.53 mM through Michaelis–Menten Chart, respectively. Low Km indicates high affinity between enzyme and substrate and high Vmax demonstrates high catalytic performance of the enzyme
Volume 13, Issue 54 (8-2015)
Abstract
Kimchi is a general term for fermented vegetables.The aim of this study was to isolate and identify the Lactic Acid Bacteria involved in spontaneous fermentation of this product used biochemical and molecular methods. In this study, after successive culture on specific media, in order to classify of 85 selected isolates that according to preliminary experiments seemed Lactic Acid Bacteria, physiological and biochemical tests were done and then fermentation of 10 different carbohydrates were performed. Based on these tests, 85 isolates were divided into 10 groups. Some isolates were selected from each group and 16S rRNA was amplified using universal primers. Diversity of lactic acid bacteria in Kimichi was as followings: Lactobacillus plantarum (41.17%), Lactobacillus fermentum (9.41%), Enterococcus faecalis (3.52%), Enterococcus faecium (7.06%), Leuconostoc citreum (2.35%), Leuconostoc mesenteroides (3.52%), Pediococcus pentosaceus (8.23%), Wisella cibaria (24.70%). This fermented product has a wide microbial diversity which originates from natural microbiota presented in the raw vegetable. Lactic acid bacteria can produce a variety of acids and enzymes which play an important role in development of unique flavor and tasteTherefore, this strains that isolated from Kimchi can be used in the food industry.
Volume 13, Issue 55 (9-2015)
Abstract
Infectious diseases created by strains of Antibiotic resistance Escherichia coli and Staphylococcus aureus, is increasing in many countries such as Iran. Therefore, many efforts are performing in order to find a new composition as replacement for antibiotics. In this experimental research, an antimicrobial effect of the ethanolic and aqueous extract of Hibiscus Sabdariffa was investigated on several strains of Escherichia coli and Staphylococcus aureus resistant to common clinical antibiotics.The extract of Hibiscus Sabdariffa was prepared using rotary. Twenty strains of Escherichia coli and Staphylococcus aureus were provided from Mashhad University of Medical Sciences. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined using Serial Dilution Method in six concentrations onto strains of Escherichia coli and Staphylococcus aureus in broth media.The results showed that Escherichia coli was resistant to antibiotics of penicillin (75.9%), erythromycin (58.3%), tetracycline (56.9%), and cefixime (37%), and Staphylococcus aureus showed resistant to antibiotics of penicillin (83.5%), cefixime (80%), erythromycin (55.6%), and tetracycline (26.1%), respectively. Also, the ethanolic extract of Hibiscus Sabdariffa has acceptable effect on antibiotic resistant strains of Escherichia coli and Staphylococcus aureus that MIC of the Hibiscus Sabdariffa ethanolic extract was 4 and 16 mg/mL for Escherichia coli and Staphylococcus aureus, respectively.The overall, the Hibiscus Sabdariffa extract has inhibitory ability on Antibiotic resistant Escherichia coli and Staphylococcus aureus strains. So, the ethanolic and aqueous extract of Hibiscus tea can be used in in pharmaceutical industry for medical treatments with perfect study.
Volume 13, Issue 55 (9-2015)
Abstract
Ice cream is a complex food colloid including fat droplet and air cells that dispersed on the relatively frosted aqueous phase. In this research work, the effect of the fat at the four level ( 2.5, 5, 7.5, 10 % ) , pekmez at the three level ( 0, 9, 18 %) , two types of hydrocolloids (Carboxyl Methyl Cellulose and balangu ) each at the four level ( 0.1, 0.3 ,0 .5 , 0.7 % ) on the viscous flow properties and total acceptance of ice cream by response surface methodology (RSM) was investigated. The flow behavior of all samples using Power law, Casson, Bingham and Herschel- Bulkley models was investigated. According to the R2 (≥0.99) and RMSE (≤0.9314) values, it is obvious that the Power law model is highly appreciated for describe flow behavior of ice cream samples. Also the Result indicated that casson plastic model was highly significant for evaluate yield stress of ice cream samples. Samples containing Balangu gum has higher consistency coefficient and yield stress and lower flow behavior index than samples containing CMC. Its worth to mention that Herschel-Bulkley model gave negative values for yield stress which has no physical meaning. Balangu seed gum (BSG) in comparison to carboxyl methyl cellulose did not have a significant effect (P > 0.05) on the total acceptance. The Desirability function has been achieved as 0.93 which, itself, indicates the accuracy of optimization. The optimum formulation has been found as following: fat of 5.6%, BSG of 0.6% , Pekmez of 18%, respectively.
Volume 13, Issue 59 (0-0)
Abstract
Fermentation is used for centuries to protect quality improvements or flavor modifications of cereals, fruits, vegetables, milk and meat. Yellow Zabol kashk (Sistani) is a grain-dairy fermented product, which is highly consumed in Sistan-Baluchistan province. The aim of this study was to isolate and identify the Lactic Acid Bacteria involved in spontaneous fermentation of this product to introduce the native strains. These strains could be used in industry or some of them may be considered as probiotic strains. The cell morphology of each strain was investigated. Then gram-positive and catalase-negative isolates were selected. In order to classify 83 selected isolates that seemed Lactic Acid Bacteria according to preliminary experiments, physiological and biochemical tests, including growth at 10°C and 45°C, 6.5% NaCl, pH=4.4 and pH=9.6, carbon dioxide production from Glucose and carbohydrates fermentation were performed. Twenty eight selected isolate identified genotypically based on 16S rRNA gene sequencing. The results showed that the isolates belong to: Lactobacillus plantarum (24.09 %), Lactobacillus helveticus (13.25 %), Lactobacillus brevis (963 %), Lactobacillus delbrueckii (18.07 %), Lactococcus lactis (13.25 %), Leuconostoc mesenteroides (9.63 %), Leuconostoc citreum (2.40 %) and Pediococcus pentosaceus (9.63 %). To identify the bacteria, especially lactic acid bacteria it is suggested using both culture and molecular-based method.
Volume 14, Issue 66 (0-0)
Abstract
Volume 14, Issue 68 (0-0)
Abstract
Volume 15, Issue 76 (0-0)
Abstract
Volume 16, Issue 87 (5-2019)
Abstract
In this study, the antimicrobial effect of Citrus aurantium essential oil on Bacillus cereus, Listeria innocua, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi and Candida albicans was evaluated. The chemical compounds, total phenol content, total flavonoids content and antioxidant potential of Citrus aurantium essential oil were determined. The results of phytochemical analysis (Ferric chloride and Shinoda) showed that, phenol, flavonoids and flavone demonstrated the presence in Citrus aurantium essential oil. Based on gas chromatography–mass spectrometry results 19 compounds identified in Citrus aurantium essential oil. Linalool (21.29 %) was the major compound in Citrus aurantium essential oil. The total phenolic content and total flavonoids content of Citrus aurantium essential oil were equal to 41.35 ± 0.46 mg GAE/g DM and 2.98 ±0.50 mg QE/g DM, respectively. The antioxidant potential based on radical Scavenging and β-carotene linoleic acid of Citrus aurantium essential oil were equal to 102.85 ± 0.60 μg/ml and 65.60% respectively. The longest and the shortest diameters of the inhibition zone at the concentration of 45 mg/ml pertained to, Listeria innocua (16.50 ±0.66 mm) and Salmonella typhi (9.80 ±0.43 mm), respectively. The minimum inhibitory concentration of the Citrus aurantium essential oil ranged from 3.125 mg/ml to 100 mg/ml, while its minimum bactericidal/fungicidal concentration ranged from 3.125 mg/ml to 200. Based on the present research, the essential oil Citrus aurantium antioxidant potential and antimicrobial activity on several food-borne pathogens tested and thus can be a good source of food industrial.
Volume 16, Issue 90 (August 2019)
Abstract
In this study, the antimicrobial effect of Ocimum basilicum essential oil with different qualitative and quantitative methods (disc diffusion, minimum inhibitory concentration and minimum bactericidal concentration) as well as the effects of its interaction with tetracycline and chloramphenicol antibiotics on Listeria innocua, Staphylococcus aureus, Bacillus cereus, Pseudomonas aeruginosa and Escherichia coli were evaluated. The chemical compounds, antioxidant activity and total phenolic content of Ocimum basilicum essential oil were measured. The results showed that Ocimum basilicum essential oil contained 31 compounds. Estragole was the major constituent of the essential oil with a percentage of 30.75. The antioxidant activity of Ocimum basilicum essential oil was 70% based on free radical scavenging capacity (DPPH). The total phenolic content of the essential oil was 29.05±0.50 mg GAE/g. The most sensitive and the most resistant bacteria with diameter inhibition zone 21.95 mm and 10.00 mm were Bacillus cereus and Escherichia coli respectively. The effects of interaction of Ocimum basilicum essential oil with tetracycline and chloramphenicol antibiotics on gram negative bacteria showed synergistic status. The minimum inhibitory concentration of the essential oil was equal to 12.5, 6.25, 6.25, 200 and 200 mg/ml for Listeria innocua, Staphylococcus aureus, Bacillus cereus, Pseudomonas aeruginosa and Escherichia coli respectively. The minimum bactericidal concentration of Ocimum basilicum essential oil was equal to 50, 12. 5, 12.5, 400 and 400 mg/ml for bacteria respectively.
Volume 16, Issue 90 (August 2019)
Abstract
Dual properties of Enterococcus on the one hand, have made them suitable and accepted bacteria as starter cultures in the production of food products and probiotics, but on the other hand, because of antibiotic resistance properties and virulence factors, has resulted in concerns about the consumer’s health. In this study, in order to evaluate the antibiotic resistance of enterococcal isolates, the disk diffusion method was used. Also, culture-based method was applied for hemolytic and gelatinase activities. The results of the inhibition zone of Enterococcus isolates against common clinical antibiotics revealed that the isolates were sensitive to Vancomycin and Ampicillin and are resistant to Kanamycin and Streptomycin. The isolates showed very little resistance to Tetracycline and Chloramphenicol and none of the isolates were resistant to all tested antibiotics. Results obtained from hemolytic activity of isolates showed that none of examined isolates were capable of blood hemolysis and consequently no clear zone of inhibition in medium containing sheep’s blood. All of isolated were γ hemolytic and they have no hemolytic activity. Gelatinase activity results confirmed that none of the isolates are able to produce clear zone in tryptone soy agar containing gelatin.
Volume 16, Issue 91 (September 2019)
Abstract
In this study, Antibacterial activity of garlic essential oil used by disk diffusion method, micro-dilution broth (minimum inhibitory concentration) and minimum bactericidal concentration (MBC) on a number of pathogenic strains (Pseudomonas aeruginosa, Escherichia coli, Listeria innocua and Staphylococcus aureus) was studied in vitro. Chemical compounds of garlic essential oil were identified by gas chromatography. The antioxidant potential, total phenol and flavonoid were determined using radical reduction capacity, Folin–Ciocalteu and aluminum trichloride colorimetric, respectively. The results showed that the minimum inhibitory concentration of garlic essential oil was 128, 128, 32 and 64 mg/ml for Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus and Listeria innocua respectively. By increasing the concentration of essential oil, the diameter of the inhibition zone increased. The highest inhibition zone with 31.70 ± 0.55 mmm diameter was due to Staphylococcus aureus. The results showed that gram-negative bacteria of Escherichia coli and Pseudomonas aeruginosa were the most resistant to garlic essential oil. The results of identification of the chemical compounds of garlic essential oil showed that the combination of di-allyl disulfide was 40.3% higher. Total phenol, flavonoids and antioxidant activity of garlic essential oil was 0.33 mg gallic acid in gram, 0.24 mg quercetin in grams and 80% respectively. The results of this study showed that garlic can be used as a potential source for the production of pharmaceutical compounds.
Volume 16, Issue 91 (September 2019)
Abstract
The objective of this study was to extract the essential oil of purple basil leaf, to identify its compounds and to investigate its antimicrobial effects on some food-borne pathogenic bacteria through different qualitative and quantitative methods, and eventually, to compare it with some antibiotics including vancomycin and gentamycin in vitro. The essential oil components were identified with GC/MS. The antimicrobial effect of basil essential oil was measured through well diffusion agar (WDA), and finally, the minimum inhibitory concentration (MIC) of the essential oil was determined using microdilution broth and triphenyl tetrazolium chloride. The results revealed that 28 identified compounds constituted 99.28% of the whole essential oil compounds. p-Allylanisole (51.64%) was the most abundant component of the essential oil. In addition, other main components such as n-Tricosane (24.83%) and Linalool (14.81%). In the wall in agar method, the mean free zone diameter was equal to 15.9 mm in the case of Gram-positive bacteria and 11.15 mm in the case of Gram-negative ones. The minimum MIC of purple basil essential oil ranged from 4.6 to 36.8 mg/ml in the case of pathogenic bacteria. Moreover, its minimum bactericidal concentration varied from 4.6 to 73.6. in conclusion, it can be said that purple basil essential oil was effective on Gram-positive bacteria at lower concentrations and could inhibit their growth.
Volume 16, Issue 91 (September 2019)
Abstract
Recently, the use of chemical and synthetic preservatives for the control of microbial pathogenesis growth has been remarkably decreased. Today, essential oils (EOs) have been considered to be natural preservatives. Fennel is among the aromatic medicinal plant that can be used as a natural preservative. In this study, to investigate the antimicrobial activity of fennel essential oil on some pathogenic microorganisms causing infection and food poisoning (Escherichia coli, Staphylococcus aureus and Candida albicans) were used disk diffusion agar (Kirby-Bauer), well diffusion agar, minimum inhibitory concentration and minimum bactericidal/fungicidal concentration methods. The interaction of fennel essential oil (FEO) with kanamycin antibiotic was investigated. The results disc diffusion agar and well diffusion agar antimicrobial assays showed that S. aureus was the most sensitive bacteria with the highest inhibition zone compared to the other microorganisms. The effects of interaction of FEO with kanamycin antibiotic on Escherichia coli, Staphylococcus aureus showed synergistic status. In general, FEO was able to prevent the growth of Escherichia coli, Staphylococcus aureus and Candida albicans “in vitro”.
Volume 16, Issue 97 (March 2020)
Abstract
In traditional medicine, Mentha pulegium was used for sinusitis treatment, gastrointestinal disorders, respiratory disorders and food detoxity. In this research, Mentha pulegium essential oil (MPEO) antibacterial activity on some foodborne pathogens was considered. For research on MPEO interaction with chloramphenicol and gentamicin antibiotic, sub-minimum inhibitory concentration was used. The results showed that in growth medium MPEO able to control pathogen microorganisms. The inhibition zone diameter (IZD), in disk diffusion method for Listeria innocua, Staphylococus aureus, Escherchia coli, Salmonella typhi, Pseudomonas aeruginosa bacteria was 16/50, 14/60, 10, 14, 10 mm respectively. The mean of IZD in well diffusion method for Listeria innocua, Staphylococus aureus, Escherchia coli, Salmonella typhi, Pseudomonas aeruginosa bacteria was 14/40, 16, 12, 14/30, 10, 10/10 mm respectively. The results showed that in combination of MPEO with gentamicin antibiotic, for all bacteria, synergistic was observed. In combination of MPEO, with chloramphenicol antibiotic in Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, synergistic state was observed. Minimum inhibitory concentration in MPEO for all bacteria was 6/25 mg/ml. In general, with regard to acquired results, one can use MPEO for pathogenic microorganisms growth control in foods.
Volume 16, Issue 97 (March 2020)
Abstract
Gamma Aminobutyric Acid (GABA) is a bioactive molecule with different physiological roles in the body that inhibits neuronal stimulation and inhibits the delivery of stress-containing messages, has a calming effect and is used to treat diseases. Different has an effective role. In the present study, the possibility of producing this amino acid by Lactococcus lactis NZ1330 was investigated. In order to optimize the fermentation process three levels of dairy sludge (5,10,15%), monosodium glutamate (0, 0.5 and 1%) were selected at 24, 48 and 72 hours after fermentation. The presence of GABA in the culture medium was investigated by thin layer chromatography. Spectrophotometric method was used to quantify the bands present in thin-layer chromatography. Optimization results at 95% significance level showed that the optimum treatment consisted of medium containing 11.2% dairy sludge, 0.7% monosodium glutamate and 70 hours fermentation at 32 ° C and under these conditions, GABA production was ppm. It's 400. Therefore, this combination of media can be used as a suitable substrate for the production of valuable GABA drug and bioactive compounds