---

Whey protein based edible coating containing 0.5, 1 and 1.5% (w/v) thyme essential oil was used for inhibition of bacterial growth of rainbow trout fillets during cold storage. Microbial analyzes (total viable counts, psychrophilic counts, lactic acid bacteria and Enterobacteriaceaeas) as well as total volatile basic-nitrogen(TVB-N) and pH measuring were performed periodically every four days. Results showed that whey protein coating containing thyme essential oil was able to inhibition of bacterial growth of fish fillets. Also the TVB-N and pH values in the coated samples were significantly (p<0.05) lower than those of non-coated treatment. Among the treatments used in this study, treatment with 1.5% thyme essential oil containing edible coating was the most effective treatment against evaluated bacteria in this study. As could extend the shelf-life of rainbow trout fillets stored at refrigerator for at least 4 days compared to control.  

---

In this study, the extract of Entromorpha intestinalis was exctracted using different solvents including water, chloroform, methanol, ethanol, hexane and acetone. Antibacterial and antifungal properties of these extracts were evaluated using a modified disk diffusion method. Microorganisms investigated in this research were included Gram-positive bacteria Lactococcus  lactis, Listeria monocytogenes, Bacillus subtilis, Gram-negative bacteria Escherichia coli and fungi Candida albicans. According to the results, the methanol and hexane extracts of this alga had the best activity against L. lactic. (p<0/05). Methanol and ethanol extracts had the best activity against L. monocytogenes (p<0/05). But for the others tested microorganisms  in this study, the extract of aceton exhibited the best inhibition zone (p<0/05). The extract of E. intestinalis possesses good antibacterial and antifungal activity against the investigated microorganisms and it could also used as a suitable natural antimicrobial compound.