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Showing 2 results for احمدی‌گاولیقی


Volume 8, Issue 2 (Spring 2019)
Abstract

Aims: The purpose of the present study was to hydrolyze Sardinella sindensis protein isolate by pancreatin enzyme and then fractionation hydrolysate based on molecular weight and finally evaluating and comparing the anti-oxidative and anti-diabetic properties of the fractions with hydrolysate.
Materials & Methods: Protein isolate from Sardinella sindensis muscle was extracted and then hydrolyzed using pancreatin enzyme in two enzyme/substrate ratio of 2.5 and 5% (W/W) for 2h. The hydrolysates were fractionated into three fractions included FPH-I (<2kDa), FPH-II (2-10kDa) and FPH-III (>10kDa) using an ultrafiltration (UF) membranes. The antioxidant and anti-diabetic activiteis of the fractions and hydrolysate were investigated.
Findings: The degree of hydrolysis increased with increasing hydrolysis time and it was significant between 30 and 60 minutes (p<0.05). FPH-III showed the highest DPPH radical scavenging activity. In terms of chelating activity on Fe2+, there was no significant difference between the fractions and hydrolysate (p>0.05). Also, FPH-III showed a better ABTS radical-scavenging activity. FPH-III had the highest inhibitory potential against α-amylase at 2.5%. In addition, the inhibitory effect of samples at 20mg/ml against α-glucosidase was less than 50%.
Conclusion: FPH-III from Sardinella sindensis protein isolate by pancreatin enzyme had the highest DPPH radical scavenging, ABTS+ activity and alpha-amylase inhibitory.
 


Volume 10, Issue 1 (1-2021)
Abstract

Polysaccharides possess diverse biological properties due to complexity of chemical structure and heterogeneity of molecular weight which could be improved through engineering approaches and chemical modifications. The objective of the present study was to determine the antioxidant and anti-diabetic effects of marine and land originated polysaccharides and explore the correlation between molecular weight and biological activities. Hence, four polysaccharides with varying size distribution and average molecular weight including fucoidan and alginate from brown seaweed Padina pavonica and polysaccharides from Flixweed (Descurainia sophia) and Fennel (Foeniculum vulgare) were subjected to hydrolysis in three levels using 0.05N hydrochloric acid at 100 C for 5, 10 and 20 minutes.  The average molecular weight ranged between 2059.5-3781.8 in fucoidan, 1774.4-2324.9 in alginate, 720.4-1373.8 in Flixweed and 5752.6-14077.5 × 103 g/mol in Fennel. The relation between molecular weight reduction and α-amylase activity inhibition was decreasing in fucoidan (52.1-32.8%) and alginate (67.6-32.2%) and increasing in Fennel (61.2-45.0%). Reduction of molecular weight enhanced the DPPH radical scavenging and ferric reducing power of fucoidan (47.9-27.8%; 0.47-0.37 Abs) and Fennel (39.0-12.7%; 0.34-0.16 Abs). The effect of molecular weight reduction was limited on antioxidant activities of alginate and Flixweed. Overall, the findings of the current study revealed that molecular weight is a determinant factor affecting bioactivities of the tested polysaccharides and thus their applications as ingredients having anti-diabetic and antioxidant functions could be possible in their native and/or hydrolyzed forms.


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