Rahmati-Yamchi, Mohammad, Rasmi, Yousef, Allameh, Abdolamir (2014). Hepatic ethoxyresorufin O-deethylase activity of liver microsomal cytochrome P-450 (CYP1A1) in young and adult rats treated with paracetamol and β-naphthoflavone. , 1(3), 195-204.
Mohammad Rahmati-Yamchi; Yousef Rasmi; Abdolamir Allameh. "Hepatic ethoxyresorufin O-deethylase activity of liver microsomal cytochrome P-450 (CYP1A1) in young and adult rats treated with paracetamol and β-naphthoflavone". , 1, 3, 2014, 195-204.
Rahmati-Yamchi, Mohammad, Rasmi, Yousef, Allameh, Abdolamir (2014). 'Hepatic ethoxyresorufin O-deethylase activity of liver microsomal cytochrome P-450 (CYP1A1) in young and adult rats treated with paracetamol and β-naphthoflavone', , 1(3), pp. 195-204.
Rahmati-Yamchi, Mohammad, Rasmi, Yousef, Allameh, Abdolamir Hepatic ethoxyresorufin O-deethylase activity of liver microsomal cytochrome P-450 (CYP1A1) in young and adult rats treated with paracetamol and β-naphthoflavone. , 2014; 1(3): 195-204.

Hepatic ethoxyresorufin O-deethylase activity of liver microsomal cytochrome P-450 (CYP1A1) in young and adult rats treated with paracetamol and β-naphthoflavone

Molecular and Biochemical Diagnosis Journal
Article 6, Volume 1, Issue 3, 2014, Pages 195-204    PDF (372.41 K)
Authors
Mohammad Rahmati-Yamchi1; Yousef Rasmi2; Abdolamir Allameh* 3
1Department of Biochemistry, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, I.R. Iran. Department of Biochemistry, Faculty of Medicine, Tabriz University of Medical Science, Tabriz, I.R. Iran
2Department of Biochemistry, Faculty of Medicine, Urumia University of Medical Science, Urumia, I.R. Iran
3Department of Biochemistry, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, I.R. Iran
Abstract
Background: Age-related differences in the ethoxyresorufin O-deethylase (EROD) activity of CYP1A1 and its inducibility in rats may determine the toxic potential of acetaminophen. This study was carried out to compare the effects of acetaminophen (APAP) and β-naphthoflavone (βNF) on CYP1A1 activity in young and adult rats.
Methods: For this purpose, young and adult rats (n = four / group) were treated with different doses of APAP. Likewise groups of young and adult rats were treated with a single dose of β-naphthoflavone (βNF, 67 mg / kg b.w). EROD was measured in microsomal fraction using resorufin as the substrate.
Results:The results showed that a single i. p. injection of APAP (25 mg / kg B.W.) failed to alter liver microsomal EROD in young and adults. Whereas, in adults treated with 250 and 450 mg APAP / kg B.W, liver CYP1A1 was elevated to about 45 and 60% respectively. The rate of CYP1A1 induction in young rats with single dose of APAP (450 mg/kg B.W) was approximately 32%. Induction in CYP1A1 was noticed 4 h after APAP injection and returned to normal levels in 24 h. The inducibility of CYP1A1 in rats treated with a toxic dose of APAP was comparable to the data obtained from animals treated βNF, 67 mg / kg b.w.
Conclusion: These results together with our previous reports indicate a similar pattern of changes in CYP1A1 in both the age-groups treated with toxic doses of APAP may suggest that the inducible CYP1A1 can equally contribute to protection against liver damage in young and adult rats.
Keywords
Age; CYP1A1; Paracetamol; EROD; Hepatotoxicity
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