Mucosa-Associated Escherichia coli Producing Cyclomodulin toxins in Colon Cancer and intestinal inflammatory Patients | ||
| Infection Epidemiology and Microbiology | ||
| Volume 11, Issue 4, Autumn 2025, Pages 317-329 PDF (550.07 K) | ||
| Document Type: Original Article | ||
| DOI: 10.52547/iem.11.4.317 | ||
| Authors | ||
| Sima Ghiasvand1; Massoud saidijam2; ghodratollah roshanaei3; Mohammad Jafari4; Mohammad Taheri1; Mohammad Sina Alikhani5; sima kazemi6; Mohammad Yousef Alikhani* 6 | ||
| 1Department of Microbiology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran | ||
| 2Research Center for Molecular Medicine, Institute of Cancer, Hamadan University of Medical Sciences, Hamadan, Iran | ||
| 3Modeling of Non communicable Diseases Research Center, Institute of Health Sciences and Technologies, Hamadan University of Medical Sciences, Hamadan, Iran | ||
| 4Department of Pathology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran | ||
| 5Student research center, Hamadan University of Medical Sciences, Hamadan, Iran | ||
| 6Infectious Disease Research Center, Avicenna Institute of Clinical Sciences, Hamadan University of Medical Sciences, Hamadan, Iran | ||
| Abstract | ||
| Background: Colorectal cancer (CRC) is the third most common cause of death. In the human intestinal tract, some Escherichia coli strains produce cyclomodulin toxins. This study aimed to determine the frequency of cyclomodulin-encoding genes in E. coli isolates from patients with CRC and inflammatory bowel disease (IBD) compared to healthy subjects. Materials & Methods: A total of 120 E. coli strains were isolated from colonic mucosa samples during 2016-2017 from Hamadan, Iran. E. coli isolates were identified using biochemical tests. Phylogroups of E. coli isolates and cyclomodulin toxin-encoding genes were identified by PCR. The results were analyzed by SPSS software. Findings: The predominant E. coli phylogroups were A (52.5%), B2 (52.5%), and A (55%) in the CRC, IBD, and healthy groups, respectively. E. coli isolates harboring the pks (32.5%) and cnf1 (27.5%) genes belonged to phylogroup B2 (p< .001), and isolates harboring the pks gene were more prevalent in CRC patients (30%). The cnf3 gene had the highest frequency (30.8%) in the cnf gene family. The highest prevalence of cnf1 (27.5%) was observed in E. coli phylogroup B2, the highest prevalence of cdt4 (58.3%) was in phylogroup B1, and the highest prevalence of cif (52.5%) was strongly related to phylogroup B2. Conclusion: The presence of cyclomodulin toxin-encoding genes in E. coli isolates was not associated with CRC, and no statistical difference was observed in the level of cyclomodulin-encoding genes in E. coli isolates. On the other hand, there is no information about the critical time of host-microbe interaction for tumorigenesis. | ||
| Keywords | ||
| E. coli: Escherichia coli; Colorectal; Cancer; Cyclomodulin; PCR | ||
|
Statistics Article View: 66 PDF Download: 15 |
||
| Number of Journals | 45 |
| Number of Issues | 2,196 |
| Number of Articles | 24,880 |
| Article View | 28,838,760 |
| PDF Download | 18,609,417 |