Biosynthesis of Copper Nanoparticles using Artemisia biennis Willd Plant Extract for Antibacterial and Anti-Biofilm Activities | ||
| Infection Epidemiology and Microbiology | ||
| Article 7, Volume 11, Issue 1, 2025, Pages 63-75 PDF (1.21 M) | ||
| Document Type: Original Research | ||
| DOI: 10.61186/iem.11.1.63 | ||
| Authors | ||
| Sadaf Fazeli; Fatemeh Rafiee* ; Atousa Ferdousi | ||
| Department of Microbiology, Shahr-e-Qods Branch, Islamic Azad University, Tehran, Iran | ||
| Abstract | ||
| Background: This research aimed to assess the antibacterial and anti-biofilm properties of copper nanoparticles (CuNPs) produced using Artemisia biennis Willd through an eco-friendly approach, targeting four pathogenic bacteria. Materials & Methods: A. biennis Willd extract with unit numbers “15.62-125” was prepared through maceration, drying, and powdering. Particle size distribution (PSD), dynamic light scattering (DLS), zeta potential, X-ray diffraction (XRD), and Fourier-transform infrared spectroscopy (FT-IR) tests were used to characterize the synthesized CuNPs. Minimum inhibitory concentrations (MICs), minimum bactericidal concentrations (MBCs), and sub-minimum inhibitory concentrations (sub-MICs) were determined to investigate the antibacterial and anti-biofilm activities of CuNPs against Staphylococcus aureus ATCC 25923, Enterococcus faecalis ATCC 29212, Escherichia coli ATCC 25922, and Klebsiella pneumoniae ATCC 13883. Findings: CuNPs synthesized using A. biennis Willd extract exhibited a brown color change with particle sizes mainly 30-40 nm by PSD. DLS indicated uniform distribution and hydrodynamic synthesis of particles with a zeta potential of -37.8. XRD and FTIR confirmed copper nanoparticle biosynthesis. The MICs of CuNPs were 15.62-62.5 μg/mL, with S. aureus and K. pneumonia revealing the highest and lowest antimicrobial drug resistance, respectively. This trend was repeated for MBCs and sub-MICs, ranging from 15.62-125 and 7.8-31.25 μg/mL, respectively. Bacterial strains were unable to form biofilms at sub-MICs. The anti-biofilm effects of CuNPs were more significant on Gram-negative bacteria. Conclusion: CuNPs synthesized using A. biennis Willd extract by a green method show promising anti-biofilm and antibacterial characteristics against bacteria, suggesting their potential for treating bacterial infections. | ||
| Keywords | ||
| Artemisia biennis Willd; Copper nanoparticles; Green synthesis; Antimicrobial drug resistance; Anti-biofilm | ||
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