TEffects of stress on hippocampal MMP2 and MMP9 activity following Brucella melitensis exposure in rats | ||
| Pathobiology Reserach | ||
| Article 7, Volume 27, Issue 1, 2024, Pages 59-66 PDF (567.58 K) | ||
| Document Type: Original Research | ||
| Author | ||
| Mahmoud Elahdadi Salmani* | ||
| Damghan University | ||
| Abstract | ||
| Introduction: Matrix Metalloproteinases (MMPs) are inflammatory mediators involved in bacterial infection and other pathological conditions. Inflammation can damage all parts of the brain, particularly sensitive areas such as the hippocampus. Chronic stress can make the brain more susceptible to infection and inflammation. This study aimed to investigate the effects of stress on the activity of MMP2 and MMP9 in the hippocampus of male Wistar rats following the administration of Brucella Melitensis (BM) vaccine. Methods: The non-stressed group received a Brucella Melitensis vaccine strain via intracebroventicular (i.c.v) and intraperitoneal (i.p) routes. The animals were subjected to heterogeneous sequential stress for nine days and/or received the same volume of Brucella Melitensis vaccine (BMV). The activity of MMP-2 and MMP-9 was measured by Gelatin Zymography. Results: The results showed that stress increased the activity of MMP9 in both the control group and the BMV, i.p., injected animals. However, stress did not affect the activity of MMP2 in either the control or the BM, i.p., inoculated conditions. Stress also increased the activity of MMP9 following i.c.v. injection of BM, without a concomitant change in the activity of MMP2 in the hippocampus. Conclusion: The study suggests that vaccination in stressed conditions could activate MMPs, which are essential players in inflammatory processes, in brain of immunized animals. Since the Brucella melitensis vaccine is used for the prophylaxis of brucellosis in small ruminants, these findings have important implications for understanding the effects of stress on the immune response to vaccination and inflammation in the brain. | ||
| Keywords | ||
| Matrix metalloproteinases; Brucella melitensis; Inflammation; hippocampus | ||
| References | ||
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