Jabbari, Reza, Hajinasrollah, Mostafa, Mohitmafi, Soroush, Rafiei, Behrouz, Ghanaati, Hossein (2023). Induced pluripotent stem cell- derived Neuronal Precursors Ameliorate Sensory and Motor Ability in Non-Human Primates Suffering Spinal Cord Injury. , 26(4), 77-85.
Reza Jabbari; Mostafa Hajinasrollah; Soroush Mohitmafi; Behrouz Rafiei; Hossein Ghanaati. "Induced pluripotent stem cell- derived Neuronal Precursors Ameliorate Sensory and Motor Ability in Non-Human Primates Suffering Spinal Cord Injury". , 26, 4, 2023, 77-85.
Jabbari, Reza, Hajinasrollah, Mostafa, Mohitmafi, Soroush, Rafiei, Behrouz, Ghanaati, Hossein (2023). 'Induced pluripotent stem cell- derived Neuronal Precursors Ameliorate Sensory and Motor Ability in Non-Human Primates Suffering Spinal Cord Injury', , 26(4), pp. 77-85.
Jabbari, Reza, Hajinasrollah, Mostafa, Mohitmafi, Soroush, Rafiei, Behrouz, Ghanaati, Hossein Induced pluripotent stem cell- derived Neuronal Precursors Ameliorate Sensory and Motor Ability in Non-Human Primates Suffering Spinal Cord Injury. , 2023; 26(4): 77-85.

Induced pluripotent stem cell- derived Neuronal Precursors Ameliorate Sensory and Motor Ability in Non-Human Primates Suffering Spinal Cord Injury

Pathobiology Reserach
Article 8, Volume 26, Issue 4, 2023, Pages 77-85    PDF (870.39 K)
Document Type: Original Research
Authors
Reza Jabbari1; Mostafa Hajinasrollah2; Soroush Mohitmafi3; Behrouz Rafiei4; Hossein Ghanaati4
1Department of Neurosurgical Science, Loghman Hakim Medical Center, Shahid Behshti University of Medial Sciences, Tehran, Iran.
2Animal Care Facility, Reproductive Biomedicine Research Center, ROYAN Institute for Biotechnology, ACECR, Tehran, Iran,
3Department of Veterinary Medicine, Karaj branch, Islamic Azad University, Karaj, Iran
4Advanced Diagnostic and Interventional Radiology Research Center (ADIR), Tehran University of Medical Sciences, Tehran, Iran
Abstract
Background: The sensory and mobility failure associated with spinal cord injury (SCI) is desperately complicated due to the pathological events that occur sequentially in consequent to the injury.

Objectives: Herein, we applied neural stem cells, derived from human iPSCs (hiPSC-NSCs), to ameliorate the behavioral complications of contusive SCI in Rhesus monkeys, in sub-acute phase.

Methods: hiPSC-NSCs were maintained and characterized in vitro for general NSCs as well as hind-limb specific gene and protein expression prior to transplantation. Moreover, Masson's trichrome staining (MTS) in addition to luxol fast blue (LFB) were performed to determine the fibrotic scar reduction and myelination respectively. Tarlov’s scale were utilized to score the motor improvement, plus, sensory perception evaluation throughout six months following the injury.

Results: hiPSC-NSCs were identified to own NSCs’ exclusive properties in vitro by SOX2, DCX and NESTIN in addition to NESTIN , PAX6 , SOX1 , HOXA2 and HOXB2 protein and gene expression. Additionally, hiPSC-NSCs caused remarkable depletion in fibrotic scar and enhance myelination; spinal shock, sensory responses, reflexes and motor function were improved over six months.

Conclusions: Our findings suggest that hiPSC-NSCs lead to promising recovery after SCI, therefore, this source of NSCs provide a therapeutic potential in clinical studies.
Keywords
hiPSC-NSCs; Spinal cord injury; Sub-acute phase; Rhesus monkey; Sensory Perception; Motor activity
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