Molecular detection of dupA-positive Helicobacter pylori from dental plaque of periodontal patients: A prospective study | ||
| Pathobiology Reserach | ||
| Article 1, Volume 26, Issue 3, 2023, Pages 7-14 PDF (947.9 K) | ||
| Document Type: Original Research | ||
| Authors | ||
| Layegheh Daliri; Amin Talebi Bazminabadi* | ||
| Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran | ||
| Abstract | ||
| Introduction: This study aimed to detect H. pylori genome in dental plaque samples and determine the frequency of the dupA after bacterial culture and PCR assay. Materials and Methods: In this prospective study, the inclusion criteria were as: age greater and equal to 20; the need for dental examination performed by a clinician, and finally no record of antibiotic uptake against H. pylori 30 days before sampling. All demographic information of included subjects such as gender, age, history of smoking, and anti-H. pylori therapy was also recorded. DNA was extracted and bacterial culturing was performed within 4 hours of sample receipt. A patient was declared positive for H. pylori when the confirmatory glmM PCR result. Results: Of 40 symptomatic subjects, thirty-five were H. pylori positive (87.5%). Among the H. pylori-positive subjects, 28 (80%) were dupA positive. Meantime, for the subjects in the healthy group, 15 cases were H. pylori positive, and among this population 6 patients (40%) were dupA positive (P value <0.05). According to this study, it has been shown that in both groups with a periodontal pocket depth of 3-4 mm and without this depth H. pylori was detectable in the dental plaques, but this rate was significantly higher in the patient group (P=0.023). Conclusions: Conclusively, the study can be the initial step in the designation of a specific bacterial gene to track the H. pylori infection and chance of associated diseases in clinical samples while discarding the endoscopic examination as the invasive method is the highest priority. | ||
| Keywords | ||
| Helicobacter pylori; dupA; dental plaque; human reservoir | ||
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