alizadeh, roghayeh, Azadmanesh, Kayhan, Arashkia, Arash, Ghanei, Mostafa (2021). Protocol for Recovery of Measles Virus from Intact/Recombinant Antigenomic DNA. , 24(1), 45-54.
roghayeh alizadeh; Kayhan Azadmanesh; Arash Arashkia; Mostafa Ghanei. "Protocol for Recovery of Measles Virus from Intact/Recombinant Antigenomic DNA". , 24, 1, 2021, 45-54.
alizadeh, roghayeh, Azadmanesh, Kayhan, Arashkia, Arash, Ghanei, Mostafa (2021). 'Protocol for Recovery of Measles Virus from Intact/Recombinant Antigenomic DNA', , 24(1), pp. 45-54.
alizadeh, roghayeh, Azadmanesh, Kayhan, Arashkia, Arash, Ghanei, Mostafa Protocol for Recovery of Measles Virus from Intact/Recombinant Antigenomic DNA. , 2021; 24(1): 45-54.

Protocol for Recovery of Measles Virus from Intact/Recombinant Antigenomic DNA

Pathobiology Reserach
Article 6, Volume 24, Issue 1, 2021, Pages 45-54    PDF (959 K)
Document Type: Original Research
Authors
roghayeh alizadeh1; Kayhan Azadmanesh* 2; Arash Arashkia2; Mostafa Ghanei3
1Baghyatollah Medical Sciences University
2Department of Molecular Virology, Pasteur Institute of Iran, Tehran, Iran
3Baghyatollah Medical Sciences University,Chemical Injuries Research Center
Abstract
Measles virus, negative-strand RNA viruses, has been known as an ideal candidate in oncolytic virotherapy. Recombinant measles virus can encode genes of interests for reaching several aims. Replication efficiency of oncolytic virus in tumoral cells is a key parameter in efficient tumor eradication. Products of P gene (P/V/C) support measle virus to circumvent IFN 1 as the main response of innate immune system against viruses. But vaccine strains used in oncolytic therapy studies comprise several mutations in their P gene sequences. These mutations affect replication efficacy which cause attenuation of measles strains applicable in vaccination. So, arming vaccine strains with the wild type P gene is helpful to reach high virus titer. Here at this study, we have expanded a protocol with details for engineering and efficient recovery of measles virus for different aims.

Keywords
Key Words: Measles Virus; Reverse Genetics; Viral Rescue; Oncolytic Virotherapy
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