Rahimkhani, A., Haghighat, S., Mahdavi, M. (2020). Application of α-Tocopherol in the Hepatitis B Vaccine Formulation in order to Improve Humoral and Cellular Immune Responses. , 23(3), 129-135.
A. Rahimkhani; S. Haghighat; M. Mahdavi. "Application of α-Tocopherol in the Hepatitis B Vaccine Formulation in order to Improve Humoral and Cellular Immune Responses". , 23, 3, 2020, 129-135.
Rahimkhani, A., Haghighat, S., Mahdavi, M. (2020). 'Application of α-Tocopherol in the Hepatitis B Vaccine Formulation in order to Improve Humoral and Cellular Immune Responses', , 23(3), pp. 129-135.
Rahimkhani, A., Haghighat, S., Mahdavi, M. Application of α-Tocopherol in the Hepatitis B Vaccine Formulation in order to Improve Humoral and Cellular Immune Responses. , 2020; 23(3): 129-135.

Application of α-Tocopherol in the Hepatitis B Vaccine Formulation in order to Improve Humoral and Cellular Immune Responses

Pathobiology Reserach
Article 2, Volume 23, Issue 3, 2020, Pages 129-135    PDF (415.31 K)
Document Type: Original Research
Authors
A. Rahimkhani1; S. Haghighat1; M. Mahdavi* 2
1Department of Microbiology, Medical Sciences Branch, Islamic Azad University, Tehran, Iran
22 Recombinant Vaccine Research Center, Tehran University of Medical Sciences, Tehran, Iran
Abstract
Aims: The common hepatitis B vaccination was designed based on alum adjuvant. This adjuvant is failed to stimulate cellular immunity so that, optimization is necessary for better stimulation of cellular immune responses. α--tocopherol is a key component of vitamin E that is considered as immunomodulatory agent to regulate immunity in order to decrease inflammation immune responses. Therefore, in the present study, the effect of α-tocopherol on the improvement of immune responses in hepatitis B vaccination was evaluated.

Materials & Methods: commercial hepatitis B vaccine was formulated with α-tocopherol at the doses of 1, 5, and 10mg. The vaccine was injected into BALB/C mice three times two weeks apart. Ten days after the last injection, blood-drawing was performed from mice groups. The levels of IFN-γ, TNF-α, IL-4, and IL-2 cytokines and IgG total antibody, as well as IgG1 and IgG2a antibody isotypes, were measured by ELISA method.

Findings: Formulated vaccine at the dose of 10mg of α-tocopherol is more potent in the significant increase of IFN-γ, TNF-α, and IL-2 cytokines and low dose result in improvement of humoral immune response.

Conclusion: Immune response of vaccine formulated with α-tocopherol was dose-dependent and at the dose of 10mg demonstrated a significant increase of Th1 response and at the dose of 1mg result in increase of antibody responses.
Keywords
Hepatitis B; Adjuvant; alum; α- Tocopherol; Vitamin E
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