Tohidi, F., Sadat, S.M., Bolhasani, A., Yaghobi, R. (2018). Immunological Evaluation of HIV-1 VLP Harboring MPER-V3 in ‎BALB/c Mice Model. , 21(2), 95-100.
F. Tohidi; S.M. Sadat; A. Bolhasani; R. Yaghobi. "Immunological Evaluation of HIV-1 VLP Harboring MPER-V3 in ‎BALB/c Mice Model". , 21, 2, 2018, 95-100.
Tohidi, F., Sadat, S.M., Bolhasani, A., Yaghobi, R. (2018). 'Immunological Evaluation of HIV-1 VLP Harboring MPER-V3 in ‎BALB/c Mice Model', , 21(2), pp. 95-100.
Tohidi, F., Sadat, S.M., Bolhasani, A., Yaghobi, R. Immunological Evaluation of HIV-1 VLP Harboring MPER-V3 in ‎BALB/c Mice Model. , 2018; 21(2): 95-100.

Immunological Evaluation of HIV-1 VLP Harboring MPER-V3 in ‎BALB/c Mice Model

Pathobiology Reserach
Article 5, Volume 21, Issue 2, 2018, Pages 95-100    PDF (419.47 K)
Authors
F. Tohidi1; S.M. Sadat* 2; A. Bolhasani2; R. Yaghobi3
1Microbiology Department, Science Agriculture & Modern Technology Faculty, Shiraz Branch, Islamic ‎Azad University, Shiraz, Iran
2Hepatitis & AIDS and Blood- borne viruses, Pasteur Institute, Tehran, Iran‎
3Shiraz Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Abstract
Aims: Developing an effective vaccine against Human Immunodeficiency Virus (HIV) is necessary. The aim of this study was the immunological evaluation of HIV-1 VLP harboring MPER-V3 in BALB/c mice model.

Materials and Methods: In the present experimental research, presenting 40 female mice, which were between 6 and 8 weeks old, were used for immunization with VLP MPER-V3. The mice were divided into 8 groups and in each group, 5 mice were considered. Injections were performed three times at three weeks intervals and subcutaneously in a volume of 100μl per mouse. Two weeks after last injection, mouse blood samples were collected by retro-orbital bleeding and immune responses were evaluated in serum for levels of total IgG and splenocytes for cytokine assay, using ELISA method. The data analysis was performed, using Mann-Whitney test and one-way analysis of variance.

Findings: The level of total antibody production was very high in all groups that had VLP alone or with adjuvant immunity, having a significant difference with the control group (p<0.05). IgG1 was the predominant isotype (Th2-biased response) in groups that had VLP injections alone or with adjuvant.

Conclusion: VLPs can stimulate the humoral immune system in mice immunized with these particles alone or formulated with adjuvant. Also, the level of production of IL-5 in the presence of the vaccine candidate as VLP increased significantly.
Keywords
vaccine; VLP; HIV-1; Immunization; BALB/c
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