Mirzaee, M., Haghighat, S., Mahdavi, M. (2018). Comparison of Immunization of Iran's Hepatitis B Vaccine, Vaccine Formulated in Montanide ISA 720 Aadjuvant and Fendrix Vaccine from GSK Company on the Balb/C Mice. , 21(1), 15-21.
M. Mirzaee; S. Haghighat; M. Mahdavi. "Comparison of Immunization of Iran's Hepatitis B Vaccine, Vaccine Formulated in Montanide ISA 720 Aadjuvant and Fendrix Vaccine from GSK Company on the Balb/C Mice". , 21, 1, 2018, 15-21.
Mirzaee, M., Haghighat, S., Mahdavi, M. (2018). 'Comparison of Immunization of Iran's Hepatitis B Vaccine, Vaccine Formulated in Montanide ISA 720 Aadjuvant and Fendrix Vaccine from GSK Company on the Balb/C Mice', , 21(1), pp. 15-21.
Mirzaee, M., Haghighat, S., Mahdavi, M. Comparison of Immunization of Iran's Hepatitis B Vaccine, Vaccine Formulated in Montanide ISA 720 Aadjuvant and Fendrix Vaccine from GSK Company on the Balb/C Mice. , 2018; 21(1): 15-21.

Comparison of Immunization of Iran's Hepatitis B Vaccine, Vaccine Formulated in Montanide ISA 720 Aadjuvant and Fendrix Vaccine from GSK Company on the Balb/C Mice

Pathobiology Reserach
Article 3, Volume 21, Issue 1, 2018, Pages 15-21    PDF (486.1 K)
Document Type: Original Research
Authors
M. Mirzaee* 1; S. Haghighat1; M. Mahdavi2
1Microbiology Department, Science & Technology Faculty, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran
2Recombinant Vaccines Research Center, Tehran University of Medical Sciences, Tehran, Iran
Abstract
Aims: Vaccination is the only approach to prevent hepatitis B virus infection, But some people do not get a good immune response to the commercial hepatitis B vaccine. The purpose of the present study was to compare the immunization of Iran's Hepatitis B vaccine, vaccine formulated in montanide ISA 720 adjuvant and Fendrix vaccine from GSK company on the Balb/c mice.

Materials and Methods: In this experimental study, 54 female inbred Balb/c mice were injected in three different formulations of vaccine subcutaneously with two-week intervals. Quantitative amount of IFN-γ, IL-4, and IL-2 cytokines and the IFN-γ/IL-4 ratio from spleen cell culture Supernatant and the anti-HBs Ag antibody level After each injection withBlood sampling were evaluated by ELISA method. Data were analyzed by Graphpad prism 6.1 software via one way analysis of variance.

Findings: The vaccine recipient groups did not show significant differences in IFN-γ, IL-2 cytokines and IFN-γ/ IL-4 ratio (p>0.05). Iranian commercial vaccine significantly increased the cytokine IL-4 in comparison with the Fendrix and HBs-MON720 groups (p<0.05). Antibody responses after the first and second injection of Fendrix and HBs-MON720 showed a significant increase in comparison with the HBS-ALUM group (p<0.05), but no significant difference was observed after the third injection (p>0.05).

Conclusion: Cytokine responses in The Fendrix vaccine and HBs-MON720 vaccine are partly similar to the Iran's Hepatitis B vaccine but after two injections, Fendrix and HBs-MON720 vaccine showed a higher antibody response.
Keywords
Alum Adjuvant; Montanide 720; Hepatitis B Vaccine; Fendrix Vaccine
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