Sadeghizadeh, Majid, Sadeghi, Iman, Bakhshinejad, Babak, Moradi Rehabdarkolaee, Hossein, Sadeghizadeh, Majid (2018). BACE1-AS non-coding RNA increases apoptosis in HAOEC endothelial cells. , 20(4), 53-66.
Majid Sadeghizadeh; Iman Sadeghi; Babak Bakhshinejad; Hossein Moradi Rehabdarkolaee; Majid Sadeghizadeh. "BACE1-AS non-coding RNA increases apoptosis in HAOEC endothelial cells". , 20, 4, 2018, 53-66.
Sadeghizadeh, Majid, Sadeghi, Iman, Bakhshinejad, Babak, Moradi Rehabdarkolaee, Hossein, Sadeghizadeh, Majid (2018). 'BACE1-AS non-coding RNA increases apoptosis in HAOEC endothelial cells', , 20(4), pp. 53-66.
Sadeghizadeh, Majid, Sadeghi, Iman, Bakhshinejad, Babak, Moradi Rehabdarkolaee, Hossein, Sadeghizadeh, Majid BACE1-AS non-coding RNA increases apoptosis in HAOEC endothelial cells. , 2018; 20(4): 53-66.

BACE1-AS non-coding RNA increases apoptosis in HAOEC endothelial cells

Pathobiology Reserach
Article 4, Volume 20, Issue 4, 2018, Pages 53-66    PDF (948.49 K)
Authors
Majid Sadeghizadeh* 1; Iman Sadeghi1; Babak Bakhshinejad1; Hossein Moradi Rehabdarkolaee2; Majid Sadeghizadeh* 1
1Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
2Department of Statistical Sciences and Operations Research, Virginia Commonwealth University, Richmond, VA 23284, U.S.A
Abstract
Objective: Heart failure is one of the most common types of cardiac diseases with high rate of morbidity and mortality, which places a large burden on society. Despite well care system, the survival rate for end-stage heart failure patients is less than 1%. Several studies have recently shown a significant association between long non-coding RNAs with different cardiac diseases.
Methods: We investigated the functional mechanism of BACE1-AS, which was previously reported to be up-regulated in heart failure patients, by assessments of gene expression analysis, cell proliferation, and apoptosis.
Results: BACE1-AS down-regulation significantly reduced expressions of cyclin inhibitors that included CDKN1A, CDKN1B, CDKN1C, CDKN2A, and CDKN2B with the exception of CDKN2C and CDKN2D. BACE1-AS over-expression increased expressions of these cell cycle genes, which implied an effect of BACE1-AS LncRNA on the cell cycle. BACE1-AS down-regulation decreased BCL-2 mRNA expression and increased BAX gene expression. BACE1-AS up-regulation increased BCL-2 and reduced BAX expressions. We did not observe any significant change in cell proliferation with BACE1-AS down-regulation, while BACE1-AS up-regulation decreased cell numbers and induced apoptosis compared with the control group (cells treated with an empty vector).
Conclusion: These results showed the important role of BACE1-AS in cell death and, accordingly, pathogenesis of heart failure. It could be considered a potential biomarker for targeting and treatment of heart failure.
Keywords
Heart failure; Biomarker; Non-coding RNA
References
Statistics
Article View: 89
PDF Download: 71
Statistics
Number of Journals45
Number of Issues2,171
Number of Articles24,674
Article View24,443,771
PDF Download17,553,708