Mohammadkarim, Alireza, Mokhtari-Dizaji, Manijhe, Kazemian, Ali, Saberi, Hazhir, Khani, Mohammad Mahdi, Tabatabaei, Seyed Mohammad, Bakhshandeh, Mohsen (2017). Investigation of morphological behavior of large vessel human endothelial cells during radiation therapy based on cytoskeleton imaging. , 20(2), 63-77.
Alireza Mohammadkarim; Manijhe Mokhtari-Dizaji; Ali Kazemian; Hazhir Saberi; Mohammad Mahdi Khani; Seyed Mohammad Tabatabaei; Mohsen Bakhshandeh. "Investigation of morphological behavior of large vessel human endothelial cells during radiation therapy based on cytoskeleton imaging". , 20, 2, 2017, 63-77.
Mohammadkarim, Alireza, Mokhtari-Dizaji, Manijhe, Kazemian, Ali, Saberi, Hazhir, Khani, Mohammad Mahdi, Tabatabaei, Seyed Mohammad, Bakhshandeh, Mohsen (2017). 'Investigation of morphological behavior of large vessel human endothelial cells during radiation therapy based on cytoskeleton imaging', , 20(2), pp. 63-77.
Mohammadkarim, Alireza, Mokhtari-Dizaji, Manijhe, Kazemian, Ali, Saberi, Hazhir, Khani, Mohammad Mahdi, Tabatabaei, Seyed Mohammad, Bakhshandeh, Mohsen Investigation of morphological behavior of large vessel human endothelial cells during radiation therapy based on cytoskeleton imaging. , 2017; 20(2): 63-77.

Investigation of morphological behavior of large vessel human endothelial cells during radiation therapy based on cytoskeleton imaging

Pathobiology Reserach
Article 5, Volume 20, Issue 2, 2017, Pages 63-77    PDF (1.3 M)
Authors
Alireza Mohammadkarim1; Manijhe Mokhtari-Dizaji* 1; Ali Kazemian2; Hazhir Saberi3; Mohammad Mahdi Khani4; Seyed Mohammad Tabatabaei5; Mohsen Bakhshandeh6
1Department of Medical Physics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
2Radiotherapy Oncology Research Center, Cancer Institute, Tehran University of Medical Sciences, Tehran, Iran
3Department of Radiology, Imam Khomeini Hospital, Tehran University of Medical Sciences, Tehran, Iran
4Department of Tissue Engineering and Regenerative Medicine, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
5Cardiovascular Engineering Lab, Faculty of Biomedical Engineering, Amir kabir University of Technology, Tehran, Iran
6Radiology Technology Department, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences and Health Services, Tehran, Iran
Abstract
Objective: Pathological changes to endothelial cells of the vessel wall may lead to vascular stenosis. In this study, we investigate damages that appear following radiotherapy in two states, single fraction and fractionation irradiation, as an effective sign of cytoskeletal and nuclei structure of vascular wall endothelial cells.
Methods: We irradiated human vein endothelial cells (HUVECs) with a Cobalt-60 therapy machine at radiation doses of 0, 2, 4, and 8 Gy. We stained the skeletal structure of the membrane and nuclei within 24 h after irradiation. This cell line received fractionation radiation therapy at doses from 0 to 8 Gy, in sub-fractions of 2 Gy, after which we stained the cytoskeleton. Morphological parameters such as area and perimeter of the cells and nuclei were determined, and we evaluated the cell shape index (CSI) for cells from each group.
Results: Increasing the irradiation dose from 0 to 8 Gy led to a significant decrease of CSI (approximately 56%) and a significant increase of nuclei shape index (approximately 85%; p<0.05). The cells’ areas and nuclei doubled (p<0.05). CSI parameter significantly decreased approximately 23% (4.5 times lower) during the radiotherapy fraction after application of the 8 Gy compared with control group cells.
Conclusion: After irradiation, we observed broken the membrane filaments that resulted in a new configuration, which led to increased cell and nuclei sizes along with alterations in the cell shape. Radiation therapy led to dose-dependent changes in morphological behavior response of the endothelial cells. Hence, it would be considered as a prognostic factor for behavior of healthy vascular cells in the process of radiotherapy.
Keywords
Endothelial cells; Cytoskeleton; morphology; Radiation therapy
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