Siahmoshteh, Fatemeh, Hamidi esfahani, Zohreh, Razzaghi-Abyaneh, Mehdi (2016). Inhibitory Effect of a Soil Isolate of Bacillus amyloliquefaciens on Aflatoxin B1 Production by Aspergillus parasiticus in Laboratory Conditions. , 19(3), 33-44.
Fatemeh Siahmoshteh; Zohreh Hamidi esfahani; Mehdi Razzaghi-Abyaneh. "Inhibitory Effect of a Soil Isolate of Bacillus amyloliquefaciens on Aflatoxin B1 Production by Aspergillus parasiticus in Laboratory Conditions". , 19, 3, 2016, 33-44.
Siahmoshteh, Fatemeh, Hamidi esfahani, Zohreh, Razzaghi-Abyaneh, Mehdi (2016). 'Inhibitory Effect of a Soil Isolate of Bacillus amyloliquefaciens on Aflatoxin B1 Production by Aspergillus parasiticus in Laboratory Conditions', , 19(3), pp. 33-44.
Siahmoshteh, Fatemeh, Hamidi esfahani, Zohreh, Razzaghi-Abyaneh, Mehdi Inhibitory Effect of a Soil Isolate of Bacillus amyloliquefaciens on Aflatoxin B1 Production by Aspergillus parasiticus in Laboratory Conditions. , 2016; 19(3): 33-44.

Inhibitory Effect of a Soil Isolate of Bacillus amyloliquefaciens on Aflatoxin B1 Production by Aspergillus parasiticus in Laboratory Conditions

Pathobiology Reserach
Article 3, Volume 19, Issue 3, 2016, Pages 33-44    PDF (851.57 K)
Authors
Fatemeh Siahmoshteh1; Zohreh Hamidi esfahani1; Mehdi Razzaghi-Abyaneh* 2
1Department of Food Science and Industry, Tarbiat Modares University, Tehran, Iran
2Department of Mycology, Pasteur Institute of Iran, Tehran, Iran
Abstract
Objective: The concern about the presence of aflatoxin and its risk for human and animal health has resulted in the introduction of different methods to eliminate or reduce this toxin. One of these methods is biological control of the fungus by other microorganisms.
Methods: We isolated a strain of Bacillus amyloliquefaciens from the soil of a pistachio orchard, Damghan, Iran. This strain was applied as a biological control to examine the inhibition of growth and toxin production by Aspergillus parasiticus NRRL2999. After 72 hours of incubation of the bacterium at 30°C, we separated the supernatant as a potential source for antifungal compounds. Different doses of the supernatant were co-cultured with the fungus suspension in glucose yeast extract broth (GYB) at 28°C for 4 days. After the incubation period, we measured the inhibition of fungal growth by dry weight assessment of the fungal mass. We performed qualitative and quantitative assessment to determine the amount of aflatoxin B1 by TLC and HPLC, respectively.
Results: Increased bacterial culture supernatant as the antagonist in fungal growth medium resulted in decreased fungal growth. AFB1 production was 2.35 ppm in control samples, whereas the amount considerably decreased in samples treated by the bacterial supernatants. The toxin reduction was dose-dependent.
Conclusion: The results of this study have shown that this bacterial strain, by taking into consideration its native origin, can be used as a biological control against aflatoxigenic fungi.
Keywords
Biological control; Aflatoxin B1; Aspergillus parasiticus; Bacillus amyloliquefaciens
References
Statistics
Article View: 118
PDF Download: 62
Statistics
Number of Journals45
Number of Issues2,188
Number of Articles24,796
Article View26,311,949
PDF Download18,128,935