Mahdavi, Mehdi, Ebtekar, Massoumeh, Azadmanesh, Kayhan, Mahboudi, Fereidoun, KhorramKhorshid, Hamidreza, Rahbarizadeh, Fatemeh, Nejadmoghaddam, Amir, Hassan, Zuhair (2010). Short Communication: Evaluation of immune responses from immunization of HIV-1 P24-Nef fusion peptide vaccine with Monophosphoryl Lipid A (MPL) in BALB/c mice. , 13(2), 79-86.
Mehdi Mahdavi; Massoumeh Ebtekar; Kayhan Azadmanesh; Fereidoun Mahboudi; Hamidreza KhorramKhorshid; Fatemeh Rahbarizadeh; Amir Nejadmoghaddam; Zuhair Hassan. "Short Communication: Evaluation of immune responses from immunization of HIV-1 P24-Nef fusion peptide vaccine with Monophosphoryl Lipid A (MPL) in BALB/c mice". , 13, 2, 2010, 79-86.
Mahdavi, Mehdi, Ebtekar, Massoumeh, Azadmanesh, Kayhan, Mahboudi, Fereidoun, KhorramKhorshid, Hamidreza, Rahbarizadeh, Fatemeh, Nejadmoghaddam, Amir, Hassan, Zuhair (2010). 'Short Communication: Evaluation of immune responses from immunization of HIV-1 P24-Nef fusion peptide vaccine with Monophosphoryl Lipid A (MPL) in BALB/c mice', , 13(2), pp. 79-86.
Mahdavi, Mehdi, Ebtekar, Massoumeh, Azadmanesh, Kayhan, Mahboudi, Fereidoun, KhorramKhorshid, Hamidreza, Rahbarizadeh, Fatemeh, Nejadmoghaddam, Amir, Hassan, Zuhair Short Communication: Evaluation of immune responses from immunization of HIV-1 P24-Nef fusion peptide vaccine with Monophosphoryl Lipid A (MPL) in BALB/c mice. , 2010; 13(2): 79-86.

Short Communication: Evaluation of immune responses from immunization of HIV-1 P24-Nef fusion peptide vaccine with Monophosphoryl Lipid A (MPL) in BALB/c mice

Pathobiology Reserach
Article 9, Volume 13, Issue 2, 2010, Pages 79-86    PDF (305.98 K)
Authors
Mehdi Mahdavi1; Massoumeh Ebtekar* 2; Kayhan Azadmanesh3; Fereidoun Mahboudi4; Hamidreza KhorramKhorshid5; Fatemeh Rahbarizadeh6; Amir Nejadmoghaddam7; Zuhair Hassan8
1Ph.D., Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
2Associate Professor, Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
3Assistant Professor, Department of Virology, Pasteur Institute of Iran, Tehran, Iran
4Associate Professor, Department of Biotechnology, Pasteur Institute of Iran, Tehran, Iran
5Associate Professor, Genetics Research Center, University of Social Welfare and Rehabilitation Sciences, Tehran, Iran
6Assistant Professor, Department of Medical Biotechnology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
7M.Sc., Department of Immunology, Pasteur Institute of Iran, Tehran, Iran
8Professor, Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
Abstract
Objective: Several vaccines against HIV have been investigated but none has been approved as an effective HIV vaccine. An approach that could induce stronger immune response against the pathogen is utilizing a multi-epitopic vaccine. This strategy was used in the design of several vaccines and resulted in improved immune responses.
Materials and Methods: In this study a multi-epitopic fusion peptide including parts of HIV-1 Nef and P24 as a vaccine candidate was injected into mice and immune humoral responses measured with total antibody and IgG sub-classes using ELISA. Also measurement of cellular immune responses through evaluation of spleen cells proliferation response using MTT and cytotoxicity by LDH were performed. Finally, the cytokine pattern of IFN-γ and IL-4 were also determined with ELISA.
Results: The results indicate that candidate vaccine stimulated mouse splenic lymphocyte proliferation response and also induced strong cytotoxicity responses. Analysis of humoral immune response has shown that the candidate vaccine has induced specific antibody production mainly of the IgG2a sub-class. Also cytokine pattern evaluation has shown that IFN-γ secretion was dominant.
Conclusion: The use of immunogen and conserved epitopes from P24 and Nef induced strong humoral and cellular immune responses and this construct could be candidate for further studies in animal models.
Keywords
HIV-1-P24-Nef; BALB/c Mouse; peptide vaccine; HIV-1 P24-Nef; Adjuvant; Monophosphoryl Lipid A
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