Malekzadeh Shafaroudi, Afsaneh, Mowla, Seyed Javad, Ziaee, Amir Mohsen, Atlasi, Yaser, Malakoutian, Mahshid, Bahrami, Ahmad Reza (2008). Evaluating the expression of Bmi-1, as a new molecular marker in diagnosis and classification of bladder tumors. , 10(0), 63-73.
Afsaneh Malekzadeh Shafaroudi; Seyed Javad Mowla; Amir Mohsen Ziaee; Yaser Atlasi; Mahshid Malakoutian; Ahmad Reza Bahrami. "Evaluating the expression of Bmi-1, as a new molecular marker in diagnosis and classification of bladder tumors". , 10, 0, 2008, 63-73.
Malekzadeh Shafaroudi, Afsaneh, Mowla, Seyed Javad, Ziaee, Amir Mohsen, Atlasi, Yaser, Malakoutian, Mahshid, Bahrami, Ahmad Reza (2008). 'Evaluating the expression of Bmi-1, as a new molecular marker in diagnosis and classification of bladder tumors', , 10(0), pp. 63-73.
Malekzadeh Shafaroudi, Afsaneh, Mowla, Seyed Javad, Ziaee, Amir Mohsen, Atlasi, Yaser, Malakoutian, Mahshid, Bahrami, Ahmad Reza Evaluating the expression of Bmi-1, as a new molecular marker in diagnosis and classification of bladder tumors. , 2008; 10(0): 63-73.

Evaluating the expression of Bmi-1, as a new molecular marker in diagnosis and classification of bladder tumors

Pathobiology Reserach
Article 8, Volume 10, Issue 0, 2008, Pages 63-73    PDF (492.26 K)
Authors
Afsaneh Malekzadeh Shafaroudi1; Seyed Javad Mowla* 2; Amir Mohsen Ziaee3; Yaser Atlasi2; Mahshid Malakoutian2; Ahmad Reza Bahrami4
1of Genetic, School of Basic Sciences, Tarbiat Modares University, Tehran, Iran
2Department of Genetic, School of Basic Sciences, Tarbiat Modares University, Tehran, Iran
3Labbafi-Nejad Urology and Nephrology Research Center, Shahid Beheshti University Medical Sciences, Tehran, Iran
4Institute of Biotechnology, Ferdowsi University, Mashhad, Iran
Abstract
Objectives: Tissue homeostasis is the result of strict regulatory mechanisms, which control self-renewal, differentiation, prevention of premature senescence and apoptosis of stem cells. Bmi-1, a Polycomb group repressor protein, represses genes that induce cellular senescence and cell death, and can contribute to cancer when improperly expressed.
Material and methods: Bladder tumoral and nontumoral samples were collected from Labbafi-Nejad hospital. RNA was extracted from each sample, reverse transcribed and amplified by RT-PCR technique, using specific primers for Bmi-1 and β2-microglobolin, as an internal control. The production and distribution of Bmi-1 protein was also examined by western blotting and immunohistochemistry technique.
Results: To clarify the role of Bmi-1 in bladder tumors, we examined the expression of Bmi-1 in tumoral and nontumoral samples. RT-PCR generated a 683 bp product, corresponding to the expected size of the Bmi-1 amplified region. The identity of the amplified fragment was then confirmed by direct DNA sequencing. The mean of expression of the Bmi-1 detected in tumoral tissues was significantly higher than the non-tumoral tissues and there is also a significant correlation between the mean of gene expression with stage of malignancy
(p < 0.05). The expression of Bmi-1 at protein level was further confirmed by western blotting and immunohistochemistry.
Conclusion: The tumor suppressor locus Cdkn2a (Ink4a/Arf locus) codes for two proteins, p16ink4a and p14arf. Ink4a and Arf are playing important roles in the retinoblastoma (pRB) and p53 pathways, respectively. Bmi-1 is a potent repressor of both pathways and hence elucidating its role in tumorigenisis is very important. Here, for the first time we are reporting the expression of Bmi-1 and its correlation with malignancy in bladder tumors.
Keywords
Bmi-1; .RT-PCR
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