Tahmasebi Fard, Zahra, Parivar, Kazem, Barzegar Yarmohammadi, Leila, Akhondi, Mohammad Mehdi, Jeddi Tehrani, Mahmoud, Rabbani, Hojjatollah (2010). Detection of murine tissues that express opticin. , 12(4), 31-38.
Zahra Tahmasebi Fard; Kazem Parivar; Leila Barzegar Yarmohammadi; Mohammad Mehdi Akhondi; Mahmoud Jeddi Tehrani; Hojjatollah Rabbani. "Detection of murine tissues that express opticin". , 12, 4, 2010, 31-38.
Tahmasebi Fard, Zahra, Parivar, Kazem, Barzegar Yarmohammadi, Leila, Akhondi, Mohammad Mehdi, Jeddi Tehrani, Mahmoud, Rabbani, Hojjatollah (2010). 'Detection of murine tissues that express opticin', , 12(4), pp. 31-38.
Tahmasebi Fard, Zahra, Parivar, Kazem, Barzegar Yarmohammadi, Leila, Akhondi, Mohammad Mehdi, Jeddi Tehrani, Mahmoud, Rabbani, Hojjatollah Detection of murine tissues that express opticin. , 2010; 12(4): 31-38.

Detection of murine tissues that express opticin

Pathobiology Reserach
Article 4, Volume 12, Issue 4, 2010, Pages 31-38    PDF (184.36 K)
Authors
Zahra Tahmasebi Fard1; Kazem Parivar2; Leila Barzegar Yarmohammadi3; Mohammad Mehdi Akhondi4; Mahmoud Jeddi Tehrani5; Hojjatollah Rabbani* 5
1Assistant Professor, Department of Biology, Islamic Azad University‮ of Roudehen Branch, Tehran, Iran
2Professor, Department of Developmental Biology, Islamic Azad University, Science and Research Unit, Tehran, Iran
3M.Sc., Monoclonal Antibody Research Center, Avicenna Research Institute, Tehran, Iran
4Associated Professor, Reproductive Biotechnology Research Center, Avicenna Research Institute, Tehran, Iran
5Associated Professor, Monoclonal Antibody Research Center, Avicenna Research Institute, Tehran, Iran
Abstract
Objective: The LRR (leucine rich proteoglycans) is a molecular recognition motif found in proteins with some roles in cell adhesion, signal transduction, DNA repair and RNA processing. Opticin is a member of this family. Takanosu et al (2001) detected messenger RNA expression of mouse opticin in the eye, heart, brain, testis, thyroid and epididymis by dot blot hybridization.
In this study, expression levels of mRNA and protein of opticin was investigated by two monoclonal antibodies which were raised against opticin peptides. By this method structure of opticin in human and mouse has been studied.
Materials and Methods: Mouse tissues including, kidney, testis, liver, lung, heart, brain, muscle, spleen and eye were isolated. Opticin expression was identified at mRNA and protein levels by RT-PCR and Western blot.
Results: PCR analysis revealed that opticin mRNA is expressed in all the tissues studied except for the lung. However, opticin protein was detected in all tissues analyzed.
Conclusion: Expression of opticin in the adult murine tissues may suggest functions other than that of putative regulation of vitreous collagen fibrillogenesis for this molecule.
Keywords
Leucine-rich repeat (LRR); Mouse tissues; Opticin
Statistics
Article View: 108
PDF Download: 37
Statistics
Number of Journals45
Number of Issues2,160
Number of Articles24,560
Article View19,398,215
PDF Download15,843,879