Jalali, Marziyeh, Rasooli, Iraj, Ahmadi Zanoos, Kobra, Jahangiri, Abolfazl, Jalali Nadooshan, Mohammadreza, Darvish Alipour Astaneh, Shakiba (2014). Immunogenicity of Amino Acid Region 7601-8140 in Biofilm Associated Protein of Acinetobacter baumannii. , 16(4), 15-26.
Marziyeh Jalali; Iraj Rasooli; Kobra Ahmadi Zanoos; Abolfazl Jahangiri; Mohammadreza Jalali Nadooshan; Shakiba Darvish Alipour Astaneh. "Immunogenicity of Amino Acid Region 7601-8140 in Biofilm Associated Protein of Acinetobacter baumannii". , 16, 4, 2014, 15-26.
Jalali, Marziyeh, Rasooli, Iraj, Ahmadi Zanoos, Kobra, Jahangiri, Abolfazl, Jalali Nadooshan, Mohammadreza, Darvish Alipour Astaneh, Shakiba (2014). 'Immunogenicity of Amino Acid Region 7601-8140 in Biofilm Associated Protein of Acinetobacter baumannii', , 16(4), pp. 15-26.
Jalali, Marziyeh, Rasooli, Iraj, Ahmadi Zanoos, Kobra, Jahangiri, Abolfazl, Jalali Nadooshan, Mohammadreza, Darvish Alipour Astaneh, Shakiba Immunogenicity of Amino Acid Region 7601-8140 in Biofilm Associated Protein of Acinetobacter baumannii. , 2014; 16(4): 15-26.

Immunogenicity of Amino Acid Region 7601-8140 in Biofilm Associated Protein of Acinetobacter baumannii

Pathobiology Reserach
Article 2, Volume 16, Issue 4, 2014, Pages 15-26    PDF (884.9 K)
Authors
Marziyeh Jalali1; Iraj Rasooli* 2; Kobra Ahmadi Zanoos1; Abolfazl Jahangiri1; Mohammadreza Jalali Nadooshan3; Shakiba Darvish Alipour Astaneh4
1Department of Biology, Shahed University, Tehran, Iran
2Department of Biology, Molecular Microbiology Research Center, Shahed University, Tehran, Iran
3Department of Pathology, Faculty of Medicine, Molecular Microbiology Research Center, Shahed University, Tehran, Iran
4Department of Microbiology, Shahed University, Tehran, Iran
Abstract
Objective: Acinetobacter baumannii (A. baumannii) is a major hospital pathogen with a high capacity to resist most common anti-microbial agents. A. baumannii is the etiologic agent for various illnesses including pneumonia, meningitis, and bloodstream infections. Biofilm associated proteins (Bap) are specific cell surface proteins essential for the formation of biofilm and play a main role in its pathogenicity. Previously, we have studied various regions of this protein. Considering different criteria, some regions were introduced as conserved and immunogenic. The immunogenicity of one of those regions pertaining to amino acids 706-1076 previously examined has shown that its expression triggers high antibody levels when injected to mice thereby protecting the animals against the bacterium. The present study examines region 4 of the Bap protein in order to validate the previous bioinformatics studies and its immunogenicity. Methods: In order to obtain immunity against this pathogen, a 1620 bp gene from Bap was amplified and cloned in pET32a. This region from Bap was cloned, expressed and verified by monoclonal antibodies. BALB/c mice were immunized by subcutaneous injection of the pure recombinant protein. Mice immune response was determined by ELISA. Results: High titer of raised antibodies implied that the recombinant protein was a strong antigen and immunogen. Conclusion: The results indicate that this protein can be a suitable choice for developing a new recombinant vaccine against A. baumannii.
Keywords
Immunogenicity; Acinetobacter baumannii; Biofilm associated protein
References
Statistics
Article View: 128
PDF Download: 81
Statistics
Number of Journals45
Number of Issues2,196
Number of Articles24,877
Article View28,766,513
PDF Download18,602,996