Tahmasebi Mirgani, Maryam, Sadeghizadeh, Majid, Sadeghizadeh, Majid, Najafi, Farhood, Mowla, Seyed Javad (2013). Dendrosomal curcumin Induced Apoptosis by Suppression of Pluripotency Genes in 5637 Bladder Cancer Cells. , 16(1), 23-39.
Maryam Tahmasebi Mirgani; Majid Sadeghizadeh; Majid Sadeghizadeh; Farhood Najafi; Seyed Javad Mowla. "Dendrosomal curcumin Induced Apoptosis by Suppression of Pluripotency Genes in 5637 Bladder Cancer Cells". , 16, 1, 2013, 23-39.
Tahmasebi Mirgani, Maryam, Sadeghizadeh, Majid, Sadeghizadeh, Majid, Najafi, Farhood, Mowla, Seyed Javad (2013). 'Dendrosomal curcumin Induced Apoptosis by Suppression of Pluripotency Genes in 5637 Bladder Cancer Cells', , 16(1), pp. 23-39.
Tahmasebi Mirgani, Maryam, Sadeghizadeh, Majid, Sadeghizadeh, Majid, Najafi, Farhood, Mowla, Seyed Javad Dendrosomal curcumin Induced Apoptosis by Suppression of Pluripotency Genes in 5637 Bladder Cancer Cells. , 2013; 16(1): 23-39.

Dendrosomal curcumin Induced Apoptosis by Suppression of Pluripotency Genes in 5637 Bladder Cancer Cells

Pathobiology Reserach
Article 3, Volume 16, Issue 1, 2013, Pages 23-39    PDF (1.17 M)
Authors
Maryam Tahmasebi Mirgani1; Majid Sadeghizadeh* 1; Majid Sadeghizadeh* 1; Farhood Najafi2; Seyed Javad Mowla1
1Department of Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
2Department of Resin and Additives, Institute for Color Science and Technology, Tehran, Iran
Abstract
Objective: The anti-cancer properties of curcumin, a poliphenol extract from the rhizome of curry, has been confirmed by many investigators. However, low levels of uptake, tissue distribution and rapid metabolism has limited its application as an anti-cancer drug. This study is aimed at increasing curcumin's water solubility due to a biodegradable, neutral and non-toxic micellar nano-carrier called dendrosome. This study intends to evaluate the role of dendrosomal-curcumin (DNC) in bladder cancer cell growth. Methods: We performed the MTT assay, flow cytometry and Annexin V-FLUOS (as an apoptosis detection kit) to evaluate cell death. The genetic mechanism of DNC-induced apoptosis was accomplished by a study of the relative expressions of OCT4A, OCT4B1, SOX-2 and Nanog using real-time PCR. Results: DNC-induced cell death complied with a time and dose-dependent paradigm in the 5637 cell line. Cell cycle analysis revealed that the number of cells increased in pre-G1 and gradually decreased in G1 and S phases. This showed the inhibitory property of dendrosomal-curcumin on DNA synthesis. Data from real-time PCR determined that expressions of OCT4A, OCT4B1, SOX-2 and Nanog could be related to 5637 cancer cell growth. Dendrosomal-curcumin significantly suppressed mRNA expression of the above mentioned genes (pConclusion: The data showed that DNC induced apoptosis by suppression of pluripotency genes in 5637 bladder cancer cells, which confirmed the useful characteristic of nano-drug in bladder cancer therapy
Keywords
Bladder cancer; Dendrosome; Curcumin; Cell-programmed death; Pluripotency genes
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