Mokhtari, Mohammad Javad, Hoseineian, Zhilla, Koohpeima, Fatemeh, Akbarzadeh, Azim, Hashemi, Mehrdad, Mahdian, Reza, Kamyab, Ahmad Reza, Mohammadi, Hadi (2013). Evaluation of Cisplatin and Cisplatin-loaded Magnetic Iron Oxide Nanoparticles on BCL2 and BAX genes in the Breast Cancer T47D Cell Line. , 15(4), 75-87.
Mohammad Javad Mokhtari; Zhilla Hoseineian; Fatemeh Koohpeima; Azim Akbarzadeh; Mehrdad Hashemi; Reza Mahdian; Ahmad Reza Kamyab; Hadi Mohammadi. "Evaluation of Cisplatin and Cisplatin-loaded Magnetic Iron Oxide Nanoparticles on BCL2 and BAX genes in the Breast Cancer T47D Cell Line". , 15, 4, 2013, 75-87.
Mokhtari, Mohammad Javad, Hoseineian, Zhilla, Koohpeima, Fatemeh, Akbarzadeh, Azim, Hashemi, Mehrdad, Mahdian, Reza, Kamyab, Ahmad Reza, Mohammadi, Hadi (2013). 'Evaluation of Cisplatin and Cisplatin-loaded Magnetic Iron Oxide Nanoparticles on BCL2 and BAX genes in the Breast Cancer T47D Cell Line', , 15(4), pp. 75-87.
Mokhtari, Mohammad Javad, Hoseineian, Zhilla, Koohpeima, Fatemeh, Akbarzadeh, Azim, Hashemi, Mehrdad, Mahdian, Reza, Kamyab, Ahmad Reza, Mohammadi, Hadi Evaluation of Cisplatin and Cisplatin-loaded Magnetic Iron Oxide Nanoparticles on BCL2 and BAX genes in the Breast Cancer T47D Cell Line. , 2013; 15(4): 75-87.

Evaluation of Cisplatin and Cisplatin-loaded Magnetic Iron Oxide Nanoparticles on BCL2 and BAX genes in the Breast Cancer T47D Cell Line

Pathobiology Reserach
Article 7, Volume 15, Issue 4, 2013, Pages 75-87    PDF (640.9 K)
Authors
Mohammad Javad Mokhtari* 1; Zhilla Hoseineian2; Fatemeh Koohpeima3; Azim Akbarzadeh4; Mehrdad Hashemi5; Reza Mahdian6; Ahmad Reza Kamyab7; Hadi Mohammadi8
1Assistant Professor, Department of Biology, Zarghan Branch, Islamic Azad University, Zarghan, Iran
2M.Sc., Department of Pilot Biotechnology, Pasteur Institute of Iran, Tehran, Iran
3Assistant Professor, Department of Operative Dentistry, School of Dentistry, Shiraz University of Medical Science, Shiraz, Iran
4Professor, Pilot Biotechnology Department, Pasteur Institute of Iran, Tehran, Iran
5Associated Professor, Department of Genetics, Tehran Medical Branch, Islamic Azad University, Tehran, Iran
6Assistant Professor, Department of Molecular Medicine, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran
7M. Sc, Department of Molecular Medicine, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran
8M. Sc, Young Researchers Club, Kermanshah Branch, Islamic Azad University, Kermanshah, Iran
Abstract
Objective: Breast cancer is the second leading cause of cancer death in women. Cisplatin is a traditional cancer drug commonly used in chemotherapy for killing cancer cells. Modulation at the mRNA levels of apoptotic related genes often correlate with the sensitivity of various types of cancer cells to chemotherapeutic agents. Nanoparticulate drug delivery systems are being developed to effectively deliver smaller doses of chemotherapeutic agents and control drug distribution in the body. In this study, we evaluate the expressions of BCL2 and BAX genes in T47D treated with cisplatin and cisplatin nanoparticles, which can result in a new approach to breast cancer therapy.
Methods: In this study, we treated T47D cells with different concentrations of cisplatin and cisplatin nanoparticles at 48 h. The IC50 was determined. We extracted RNA by using RNX solution, after which cDNA was synthesized. The precise primers for the BCL2, BAX and TBP genes were designed by specific software. The quantity of BCL2 and BAX gene expression compared to TBP gene (reference gene) was analyzed using real-time PCR.
Results: BCL2 and BAX gene expression levels in T47D cells treated by cisplatin were 0.7 (BCL2) and 1.48 (BAX); in T47D cells treated with cisplatin-loaded nanoparticles, the gene expressions were 0.03 (BCL2) and 2.41 (BAX).
Conclusion: In this study, the results have shown that cisplatin-loaded nanoparticles are effective anticancer agents. Cisplatin nanoparticles induce apoptosis in human breast cancer cell lines. We have shown that cisplatin nanoparticles strongly increased cytotoxicity in comparison to the free drug in the T47D cell line.
Keywords
Breast Cancer; Cisplatin; Nanoparticle; Programmed Cell Death Gene
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