Hashemi, Atieh, Roohvand, Farzin, Ghahremani, Mohammad-hossein, Edalat, Rosita (2012). Evaluation of the Effects of Interferon on Stability of Control Genes in Huh-7 and HepG2 Cells. , 15(2), 73-85.
Atieh Hashemi; Farzin Roohvand; Mohammad-hossein Ghahremani; Rosita Edalat. "Evaluation of the Effects of Interferon on Stability of Control Genes in Huh-7 and HepG2 Cells". , 15, 2, 2012, 73-85.
Hashemi, Atieh, Roohvand, Farzin, Ghahremani, Mohammad-hossein, Edalat, Rosita (2012). 'Evaluation of the Effects of Interferon on Stability of Control Genes in Huh-7 and HepG2 Cells', , 15(2), pp. 73-85.
Hashemi, Atieh, Roohvand, Farzin, Ghahremani, Mohammad-hossein, Edalat, Rosita Evaluation of the Effects of Interferon on Stability of Control Genes in Huh-7 and HepG2 Cells. , 2012; 15(2): 73-85.

Evaluation of the Effects of Interferon on Stability of Control Genes in Huh-7 and HepG2 Cells

Pathobiology Reserach
Article 7, Volume 15, Issue 2, 2012, Pages 73-85    PDF (1.39 M)
Authors
Atieh Hashemi1; Farzin Roohvand* 2; Mohammad-hossein Ghahremani3; Rosita Edalat4
1Ph.D. Candidate, Department of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran, Iran
2Assistant Professor, Department of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran, Iran
3Pharmacology and toxicology department, Pharmacology School, University of Tehran, Tehran, Iran
4M.Sc., Department of Virology, Pasteur Institute of Iran, Tehran, Iran
Abstract
Objective: Understanding gene expression variations by using RNA transcript analysis methods during hepatic viral protein interactions with the IFN pathway in hepatic cell lines has recently gained importance. One of the most powerful techniques in gene expression quantification is quantitative real-time RT-PCR. Reference genes used as normalizer in this method may be affected across various experimental conditions or treatments. Hence, in the present study, the influence of IFN-a treatment on the mRNA levels of common reference genes including ACTB, GAPDH, TBP, HPRT1 and HMBS was evaluated in Huh-7 or HepG2 cell lines. Methods: Cells were treated with different concentrations of IFN-a. Then, using geNorm and NormFinder programs, we evaluated the expression stabilities of the above prominent reference genes in three sample groups that included each hepatic cell line and the total data sets. Results: HPRT-1 and GAPDH were the most stable reference genes in the Huh-7 cell line, whereas ATCB, HMBS and GAPDH were the most stable in the HepG2 cell line. TBP was one of the least stable reference genes in the three studied groups. Conclusion: This investigation will provide appropriate reference genes for standardization of quantitative real-time PCR data in an IFN-a stimulated model of hepatocyte cell lines.
Keywords
Huh-7; HepG2; IFN-; Control Genes
References
Statistics
Article View: 123
PDF Download: 55
Statistics
Number of Journals45
Number of Issues2,171
Number of Articles24,674
Article View24,395,653
PDF Download17,535,420