Yazdani, Laleh, Ravanshad, Mehrdad, Khanlari, Zahra, Yaghoobi, Ramin (2012). Application of Semi-nested PCR RFLP for Detection and Genotyping GBV-C among HBV-infected Patients. , 15(1), 81-91.
Laleh Yazdani; Mehrdad Ravanshad; Zahra Khanlari; Ramin Yaghoobi. "Application of Semi-nested PCR RFLP for Detection and Genotyping GBV-C among HBV-infected Patients". , 15, 1, 2012, 81-91.
Yazdani, Laleh, Ravanshad, Mehrdad, Khanlari, Zahra, Yaghoobi, Ramin (2012). 'Application of Semi-nested PCR RFLP for Detection and Genotyping GBV-C among HBV-infected Patients', , 15(1), pp. 81-91.
Yazdani, Laleh, Ravanshad, Mehrdad, Khanlari, Zahra, Yaghoobi, Ramin Application of Semi-nested PCR RFLP for Detection and Genotyping GBV-C among HBV-infected Patients. , 2012; 15(1): 81-91.

Application of Semi-nested PCR RFLP for Detection and Genotyping GBV-C among HBV-infected Patients

Pathobiology Reserach
Article 8, Volume 15, Issue 1, 2012, Pages 81-91    PDF (789.59 K)
Authors
Laleh Yazdani1; Mehrdad Ravanshad* 1; Zahra Khanlari1; Ramin Yaghoobi2
1Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
2Shiraz Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Abstract
Objective: GBV-C is a virus transmissible via blood transfusion and sexual routes. Because of the shared transmission routes in GBV-C and hepatitis B virus (HBV) we have attempted to detect GBV-C in HBsAg-positive patients using the more sensitive semi-nested PCR. We used restriction fragment length polymorphism (RFLP) to determine genotype.
Methods: A total of 100 serum samples were collected from HBsAg-positive patients from 1389-1390. After designing specific primers and optimizing the semi-nested PCR, sequences of PCR products were analyzed by Neb Cutter software. Restriction enzyme sites were determined and suitable enzymes selected for RFLP.
Results: Semi-nested PCR shows acceptable sensitivity for the detection of GBV-C and its genotype determination. This technique is more affordable than other techniques.
Conclusion: There appears to be a high rate of GBV-C infection among Iranian HBV patients. In this study, the majority of patients co-infected with GBV-C were of HBV genotype 2, which is similar to the pattern seen in the US and Europe.
Keywords
RFLP; Semi Nested PCR; HBV; Genotyping; GBV-C; HBs-Ag Positive; Semi-Nested PCR
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